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Reproduction Abstracts (2014) 1 P303 | DOI: 10.1530/repabs.1.P303

WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)

Effect of progesterone supplementation on endometrial receptivity for implantation in the pig

Agnieszka Blitek & Magdalena Szymanska


Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland.


Progesterone (P4) produced by the corpus luteum (CL) is required for the attainment of endometrial receptivity and further conceptus implantation. Induction of puberty with PMSG/hCG in gilts results in lower P4 concentration in blood serum, that is accompanied by decreased endometrial expression of genes involved in conceptus-maternal interactions. Therefore, the present study aimed to determine the effect of P4 supplementation in pregnant gilts on endometrial preparation for implantation. Prepubertal gilts were induced to ovulate with 750 IU PMSG / 500 IU hCG, and inseminated. On days 3 to 10 of pregnancy, gilts received i.m. injections of corn oil vehicle (n=4) or P4 (50 mg/100 kg; n=4). Blood samples were collected each day beginning form day 1 of pregnancy to analyze the profile of P4 concentration using RIA method. Animals were slaughtered on day 12 of gestation. Uterine luminal flushing (ULF) was examined for E2, PGE2 and IL6 concentrations with RIA/EIA. Uterine horns were weighted and endometrial expression of PTGS2, HOXA10, LIF, TGFβ1 and IL6 mRNA was determined using qPCR. P4-treated gilts demonstrated greater concentrations of P4 in peripheral blood serum on days 8 to 10 and had heavier uteri after slaughter (P<0.05). The concentration of E2 in ULF increased, but PGE2 decreased, after P4 treatment (P<0.05). Neither IL6 content in ULF, nor endometrial IL6 mRNA expression differ between groups. Similarly, PTGS2, HOXA10 and TGFβ1 mRNA expression was not affected by P4 injections. Summarizing, P4 supplementation had no significant impact on the expression of markers of endometrial receptivity in the pig. Supported by NSC grant 2011/01/B/NZ9/07069.

Volume 1

World Congress of Reproductive Biology 2014

Edinburgh, UK
02 Sep 2014 - 04 Sep 2014

World Congress of Reproductive Biology 

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