SRF2015 POSTER PRESENTATIONS (1) (56 abstracts)
Differential gene expression in granulosa–lutein cells from women with polycystic ovaries is independent of the dose of FSH given for ovarian stimulation
Jocelyne Velupillai 1 , Avi Lerner 1 , Georgios Christopoulos 2 , Maneshka Liyanage 2 , Stuart Lavery 2 , Geoffrey Trew 2 , Mhairi Laird 1 , Jocelyn M Mora 1 , Victoria Tsui 1 , Kate Hardy 1 & Stephen Franks 1
1Imperial College London, London, UK; 2IVF Hammersmith, London, UK.
Polycystic ovary syndrome (PCOS) affects over 5% of women of reproductive age. It is associated with an ovulatory infertility, menstrual disturbances as well as metabolic abnormalities including obesity and insulin resistance. PCOS has a strong genetic basis but studies of ovarian gene expression in PCOS are limited by the difficulty in obtaining tissue samples from women with and without PCOS. For that reason, most studies have been performed in granulosa–lutein (GL) cells obtained as a by-product of egg collection for IVF. However, none of the studies to date has taken into account the effect of the dose of exogenous FSH given to stimulate multiple follicle development, an important issue considering that modern stimulation regimens typically employ lower doses of FSH for superovulation in women with PCO (Hardy et al., Hum Reprod 1995 10 2125–35). The aim of this study was to examine differential expression of key genes involved in gonadotrophin action and steroidogenesis in GL cells from women with normal ovaries (n=5) and two groups of women with PCO, those with polycystic ovary syndrome (n=9) and those with PCO morphology who had a regular cycle (n=6) using quantitative PCR. As shown previously (Catteau-Jonard et al., JCEM 2008 93 4456–61), AMH (threefold, P<0.05) and FSHR (two- to threefold, P<0.05) were upregulated, but a novel finding was the reduction in expression of CYP11A1 (P<0.001) in GL cells from both PCOS and PCO groups. Importantly, although the total dose of FSH used for superovulation in women with PCO/PCOS was typically half that in controls, there was no relationship between levels of gene expression and dose of FSH in individual subjects. In conclusion, we demonstrate distinctive gene expression profiles in granulosa cells from women with PCOS and PCO which are independent of the dose of FSH used for ovarian stimulation.
Article tools
My recent searches
My recently viewed abstracts
Authors
Reproduction Abstracts
ISSN 2052-1472 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more