Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p095 | (1) | WCRB2014

The effect of vitrification on subsequent mouse embryo development and postnatal health

Khalif Raja Ili Airina Raja , Smyth Neil , Fleming Tom , Velazquez Miguel Abraham , Sheth Bhav , Smith Stephanie

Short-term effects of cryopreservation on embryo development, survival and after embryo transfer (ET), resulting pregnancy rate have been documented. However, long-term effects on offspring remain relatively unexplored.Materials and methods: Using a mouse model, we investigated the effect of vitrification on blastocyst trophectoderm (TE) and inner cell mass (ICM) cell numbers by differential staining and postnatal growth and cardiovascular and metabolic ...

ra0001p096 | (1) | WCRB2014

Insulin and branched-chain amino acid depletion during mouse in vitro preimplantation development alters postnatal growth and cardiovascular physiology

Velazquez Miguel Abraham , Sheth Bhav , Marfy-Smith Stephanie , Eckert Judith , Fleming Tom

Introduction: Maternal depletion of insulin in serum and branched-chain amino acids (BCAA) in uterine luminal fluid occurs in response to dietary protein undernutrition during the periconceptional period which leads to adverse offspring growth and cardiovascular phenotype (e.g. high blood pressure). This study aimed to determine whether insulin and/or BCAA depletion might be inductive in vivo factors for postnatal phenotype using an in vitro embryo culture mo...

ra0002o014 | SRF Student Prize Session | SRF2015

Maternal protein restriction around conception reduces the neural stem cells during mouse fetal brain development and alters neuronal differentiation during gestation

Gould Joanna Mary , Fleming Tom , Airey Chris J , Marfy-Smith Stephanie Jane , Willaime-Morawek Sandrine

Maternal malnutrition during pregnancy is detrimental to fetal development and increases the risk of many chronic diseases in later life i.e. neurological consequences such as increased risk of schizophrenia. Previous studies have shown maternal protein malnutrition during pregnancy and lactation compromises brain development in late gestation and after birth, affecting structural, biochemical, and pathway dynamics with lasting consequences for motor and cognitive function. Ho...

ra0001p025 | Stem cells and reprogramming | WCRB2014

The role of maternal low protein diet on neural stem cells and neurogenesis in the offspring brain?

Airey Chris J , Smith Phoebe J , Gould Joanna M , Marfy-Smith Stephanie J , Fleming Tom P , Willaime-Morawek Sandrine

Globally, malnutrition is the single greatest threat to public health. Maternal malnutrition during pregnancy is detrimental to foetal development and increases the risk of many chronic diseases in later life. Neurological consequences include increased risk of schizophrenia and abnormal anxiety-related behaviour. Previous studies have shown that maternal protein restriction has a negative effect on foetal brain development in vivo and we previously showed an effect o...