Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0003o016 | SRF Student Prize Session | SRF2016

Exaggerated metabolic changes during puberty precede adult obesity and hyperlipidemia in an ovine model of Polycystic Ovary Syndrome (PCOS)

Siemienowicz Katarzyna , Coukan Flavien , Lerner Avigdor , Franks Stephen , Rae Mick , Duncan Colin

Women with Polycystic Ovary Syndrome are at increased risk of developing insulin resistance, obesity and dyslipidemia. Amplified metabolic perturbations during puberty may be a central factor contributing to metabolic phenotype of adult PCOS. Using a clinically realistic ovine model of PCOS we reported hyperinsulinaemia and early fatty liver changes, with no difference in body weight and adiposity, in adolescence.Here we aimed to further examine metaboli...

ra0003o026 | Oral Communications 3: Environmental and programming effects on reproduction | SRF2016

Fetal androgens determine adult pancreatic function

Rae Mick , Ramaswamy Seshadri , Grace Cathal , Mattei Ashley , Siemienowicz Kasia , Brownlee Bill , MacCallum Janis , McNeilly Alan , Duncan Colin

Introduction: Maternal androgen excess in pregnant sheep programmes a PCOS-like phenotype in female offspring. We previously demonstrated a hyperinsulinaemic offspring phenotype in response to maternal androgen excess, but such regimens also increase estradiol concentrations, and may effect adrenal steroidogenesis, hence the role of androgens remains unclear.Materials and Methods: To examine contributions of different steroid classes, and to determine if...

ra0001p135 | (1) | WCRB2014

Interaction between galectin-1 and glycoconjugates is involved in hCG-mediated gene expression in cultured human luteinized granulosa cells

Nio-Kobayashi Junko , Iwanaga Toshihiko , Duncan W Colin

Introduction: The corpus luteum (CL) is a temporal endocrine organ secreting large amount of progesterone essential for the establishment and maintenance of pregnancy. The CL undergoes luteolysis in a non-fertile cycle, while the CL is rescued from luteolysis by human chorionic gonadotrophin (hCG) secreted from the conceptus during pregnancy. We recently revealed that a β-galactoside-binding lectin, galectin-1, plays important roles in the regulation of luteal rescue in t...

ra0002p033 | (1) | SRF2015

Functional evaluation of miRNAs during the ovarian follicular/luteal transition

Mohammed Bushra T , Duncan W Colin , Donadeu Francesc X

Little is known about the involvement of miRNAs in luteal development. Cattle provide a convenient model to study ovarian physiology in monovular species. Our previous microarray studies in bovine showed significant upregulation of miR-96 and miR-132 in luteal relative to follicular tissues. In the present study we used an in vitro model of forskolin-induced follicular granulosa cell luteinisation, transfection with specific locked nucleic acid inhibitors or mimics of...

ra0001p013 | Testis | WCRB2014

Exogenous androgens reduce the expression of INSL3, a hormone involved in normal testicular descent, in fetal Leydig cells

Duncan W Colin , Connolly Fiona , Boswell Lyndsey , Burt Graeme , McNeilly Alan S , Rae Mick

Introduction: Hypothalamo–pituitary–testicular feedback is intact in the ovine fetus. Exogenous testosterone suppresses fetal LH and testicular steroidogenesis to maintain normal circulating testosterone concentrations. INSL3 is a non-steroidal hormone secreted by the fetal testis whose roles include facilitating testicular descent. We hypothesised that the homeostatic response to increased androgens would have a detrimental effect on fetal INSL3 expression....

ra0001p023 | Ovary | WCRB2014

Functional evaluation of miRNAs during bovine ovarian follicular/luteal development

Mohammed Bushra , Sontakke Sadanand D , Duncan W Colin , Donadeu Francesc X

Little is known about the involvement of miRNAs during terminal follicle differentiation in the monovular ovary. This study aimed to characterise miRNAs involved in the follicle-luteal transition in bovine. Microarray analyses were performed on RNA from ovulatory-size follicles (n=6) and early corpora lutea (n=6) obtained at an abattoir. Exiqon’s miRCURY LNA microRNA Array, sixth generation was used and results were validated by qPCR. A total of ten and ...

ra0001p330 | (1) | WCRB2014

Metabolic reprogramming may underpin the aetiology of endometriosis

Young Vicky J , Brown Jeremy K , Maybin Jacqueline A , Duncan W Colin , Horne Andrew W

Transforming growth factor beta (TGFβ) can induce The Warburg-effect, or aerobic glycolysis, in tumorigenesis. Energy-rich lactate, a by-product of glycolysis, ‘feeds’ tumour cells and increases apoptosis resistance, angiogenesis, and invasion, fuelling tumour progression and metastasis. As TGFβ is increased in the peritoneal fluid of women with endometriosis, we asked, is there a Warburg-like effect at play in endometriosis.Peritonea...

ra0002o019 | SRF Student Prize Session | SRF2015

Obesity in PCOS: a consequence of prenatally programmed reduced energy expenditure

Siemienowicz Katarzyna Joanna , Rae Mick , Lerner Avi , Franks Stephen , Duncan W Colin

Polycystic ovary syndrome, a common endocrine condition affecting up to 10% of women of reproductive age, is associated with an increased risk of developing insulin resistance and obesity. Obesity is associated with increased energy intake or reduced energy expenditure. In this context, postprandial thermogenesis (PPT), is an important constituent of energy expenditure.Our lab utilizes a clinically realistic ovine model of PCOS, where pregnant Scottish G...

ra0002p031 | (1) | SRF2015

Does maternal progesterone supplementation in early pregnancy affect fetal development?

Mareckova Magda , Siemienowicz Katarzyna Joanna , Wang Yili , Rae Mick , Duncan W Colin

Some adult diseases are programmed in utero by fetal exposure to abnormal concentrations of steroid hormones. Threatened miscarriage in early pregnancy is treated by progesterone in many countries although robust evidence of efficacy is lacking. We hypothesised that increased progesterone concentrations may alter fetal development. In a small pilot study, using a pregnant sheep model, we administered 200 mg progesterone twice weekly from d20–d75 of gestation and ...

ra0001p321 | (1) | WCRB2014

Fetal overexposure to androgens, not estrogens, leaves a permanent legacy in terms of adrenal function in offspring

Ramaswamy Seshadri , Grace Cathal , Howie Forbes , Connolly Fiona , Konovalova Julia , McNeilly Alan S , Duncan W Colin , Rae Mick

Introduction: Overexposure to androgens during fetal life creates offspring with a polycystic ovary syndrome (PCOS)-like phenotype. Whether this phenotype is attributable to androgens during fetal life, or estrogens via maternal/placental metabolism, remains uncertain. The potential for in utero steroidal disruption of adrenal development and function is also unclear. We have examined the adrenal during development and adulthood in response to in utero androg...