Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p096 | (1) | WCRB2014

Insulin and branched-chain amino acid depletion during mouse in vitro preimplantation development alters postnatal growth and cardiovascular physiology

Velazquez Miguel Abraham , Sheth Bhav , Marfy-Smith Stephanie , Eckert Judith , Fleming Tom

Introduction: Maternal depletion of insulin in serum and branched-chain amino acids (BCAA) in uterine luminal fluid occurs in response to dietary protein undernutrition during the periconceptional period which leads to adverse offspring growth and cardiovascular phenotype (e.g. high blood pressure). This study aimed to determine whether insulin and/or BCAA depletion might be inductive in vivo factors for postnatal phenotype using an in vitro embryo culture mo...

ra0003o002 | Oral Communications 1: Ovary | SRF2016

The effect of folic acid supplementation during the juvenile pubertal period or adulthood on gene expression in the ovary

Escarate Reyna Penailillo , Burton Mark , Burdge Graham , Eckert Judith , Fleming Tom , Lillycrop Karen

Introduction: Women with mutations in BRCA1 gene have an increased lifetime risk of ovarian cancer. Many studies but not all have shown that an adequate folate intake is protective against many cancers including ovarian, but recent studies have shown that high levels of folic acid (FA) supplementation can promote cancer risk. The aim of this study is to determine FA impact on the expression of cancer related genes in the ovary such as OCT4 a pluripotency factor, BRCA1 related ...

ra0003o020 | SRF Student Prize Session | SRF2016

Maternal high fat diet (HFD) in the adult offspring brain modifies cell density and neuronal density

Pedraza Diego Ojeda , Jane-Coupe Kate , Earl Megan , Hutton Oliver , Eckert Judith , Willaime-Morawek Sandrine

Introduction: An upward trend in maternal obesity is rising every year. Different data suggest that maternal obesity during gestation may have effects on a high risk of children of developing physiological and psychological dysfunctions in later life. Animal models suggest that a maternal high fat diet (HFD) during pregnancy could have enduring consequences on brain structure and development in the offspring. Our aim is to evaluate the effects of maternal HFD on both offspring...

ra0003o028 | Oral Communications 4: Early Development 2 | SRF2016

The effect of decidual stromal cells on immune cells in the first trimester of pregnancy

James-Allan Laura , Cartwright Judith , Whitley Guy

Introduction: Decidualisation occurs in the secretory phase of the menstrual cycle, transforming endometrial stromal cells to secretory decidual stromal cells (DSC). Extensive cross-talk occurs between DSC and immune cells. In the first trimester of pregnancy the decidua is rich with maternal leukocytes, made up of ~70% NK cells and ~20% macrophages. The number of NK and macrophage cells increases during decidualisation and into the first trimester of pregnancy. Decidual NK (d...

ra0001p297 | (1) | WCRB2014

Does decidual macrophage polarisation affect trophoblast behaviour in co-culture studies?

Buckley Rebecca , Dumitriu Ingrid , Whitley Guy S , Cartwright Judith

Introduction: Decidual macrophages constitute the second largest leukocyte cell type in the decidua during early pregnancy, and have been implicated in the regulation of extravillous trophoblast (EVT) invasion. Macrophage cells in the decidua are thought to have an anti-inflammatory M2 phenotype and the presence of macrophages with a more pro-inflammatory M1 phenotype has been associated with preeclampsia. The aim of this study was to determine whether decidual macrophage pola...

ra0002o017 | SRF Student Prize Session | SRF2015

Decidual macrophages become increasingly immunosuppressive during early pregnancy and an altered activation state may be associated with a higher risk of preeclampsia

Buckley Rebecca , Dumitriu Ingrid , Whitley Guy S , Cartwright Judith

Human pregnancy represents a unique immunological environment. However, very little is known about the role of uterine decidual macrophages (dMs) in early human pregnancy. The monocytic cell line (THP1) was activated and polarised to a pro-inflammatory M1 or immunosuppressive M2 M phenotype and the conditioned media was used to treat fetally-derived trophoblast cells. Treatment with M1 conditioned media was found to significantly reduce trophoblast motility in vitro, ...

ra0003p033 | (1) | SRF2016

Determining the effect of extra-villous trophoblast cells on spiral artery remodelling: What is the role of MMP10?

Sayer Ben , Ashton Sandra , Cartwright Judith E , Whitley Guy

Background: Spiral artery remodelling is crucial for a successful pregnancy. In a healthy human pregnancy, cells of the placenta called extravillous trophoblasts (EVT) invade into the decidua and interact with the endothelial cells (EC) and vascular smooth muscle cells (VSMC) of the maternal spiral arteries (SA) in a process known as spiral artery remodelling. This process results in the development of low-resistance, dilated SAs that increase the blood supply to the developin...

ra0001p298 | (1) | WCRB2014

Decidual stromal cell regulation of trophoblast in first trimester pregnancies with normal and impaired spiral artery remodelling

James-Allan Laura , Wallace Alison E , Whitley Guy S , Cartwright Judith

Introduction: Decidualisation of the endometrium is the differentiation of uterine stromal cells into specialised secretory decidual stromal cells (DSC) primed for pregnancy. If pregnancy occurs, DSC control and regulate fetal trophoblast invasion. Pre-eclampsia is a disorder associated with inadequate trophoblast invasion and spiral artery (SA) remodelling. Uterine artery Doppler resistance index (RI) in the first trimester of pregnancy measures the extent of SA remodelling; ...