Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p197 | (1) | WCRB2014

Trophectoderm stem cells to model the effect of altered periconceptional diet on embryos

Chang Yi-Lung , Fleming Tom , Smyth Neil

Introduction: Poor maternal diet during development induces physiological, metabolic and anatomical alterations in offspring. These may increase immediate survival chances, but can enhance the risk of disease in later life. Maternal low protein diet (LPD) exclusively in pre-implantation development induces changes in endocytosis, postnatal hypertension, cardiovascular dysfunction and alters behaviour in our mouse model. We have previously shown changes in the embryonic stem ce...

ra0003o017 | SRF Student Prize Session | SRF2016

Derivation and use of mouse embryonic stem cell lines as model for mechanistic analysis of periconceptional developmental programming

Khurana Pooja , Cox Andy , Fleming Tom , Smyth Neil

Introduction: The developmental origins of health and disease (DOHaD) concept proposes that maternal environment during pregnancy may influence offspring health and predispose to chronic disease risk in later life. Our previous mouse in vivo studies showed that maternal low protein diet (LPD) and advanced maternal age (AMA) programme the preimplantation embryo to adult disease, notably cardiometabolic dysfunction. To test this hypothesis and understand the mechanistic...

ra0001p090 | (1) | WCRB2014

Expression pattern and function of claudins during tight junction maturation in the mouse pre-implantation embryo

Sheth Bhavwanti , Cox Andrew L , Greenslade Karen J , Alexopoulos Orpheas , Fleming Tom P

Introduction: The pre-implantation embryo has two distinct cell lineages at the blastocyst stage. An outer epithelial monolayer called trophectoderm and the non-polar inner cell mass. Tight junctions (TJ) form continuous intercellular contacts between neighbouring trophectoderm cells and are crucial in establishing a blastocoele cavity. TJs are composed of several transmembrane proteins including Claudins, a large family of proteins that play a central role in the formation of...

ra0001p110 | (1) | WCRB2014

Epigenetic regulation of Gata-6 transcription factor expression in mouse primitive endoderm extra-embryonic lineage induced by maternal protein restricted diet

Sun Congshan , Sheth Bhav , Smyth Neil , Cox Andrew L. , Denisenko Oleg , Fleming Tom

Introduction: Maternal low protein diet exclusively during preimplantation mouse development (Emb-LPD) induces compensatory changes in extra-embryonic lineages including primitive endoderm (PE) to enhance nutrient delivery during later gestation, for example, stimulation of endocytosis. Whilst such responses protect fetal growth, adult offspring exhibit increased cardiometabolic disease risk. Here, we investigate epigenetic mechanisms underlying maternal dietary programming, u...

ra0002o008 | Oral Communications 1: Embryo and Implantation | SRF2015

The effect of IVF and embryo culture on mouse development and adult health

Aljahdali Anan , Raja Khalif Raja Ili Airina , Velazquez Miguel Abraham , Sheth Bhav , Smyth Neil , Fleming Tom

Several million babies have been born worldwide since IVF and assisted-reproductive technologies (ART) became available. However, reports link IVF techniques with adverse short- and long-term health outcomes.1,2,3 Using a mouse model, we investigated the effect of IVF on the total cell number in blastocyst stage embryos and the postnatal health of offspring.Methods: Experimental groups; (NM) C57/BL6 non-superovulated females naturally mated wi...

ra0001p095 | (1) | WCRB2014

The effect of vitrification on subsequent mouse embryo development and postnatal health

Khalif Raja Ili Airina Raja , Smyth Neil , Fleming Tom , Velazquez Miguel Abraham , Sheth Bhav , Smith Stephanie

Short-term effects of cryopreservation on embryo development, survival and after embryo transfer (ET), resulting pregnancy rate have been documented. However, long-term effects on offspring remain relatively unexplored.Materials and methods: Using a mouse model, we investigated the effect of vitrification on blastocyst trophectoderm (TE) and inner cell mass (ICM) cell numbers by differential staining and postnatal growth and cardiovascular and metabolic ...

ra0001p096 | (1) | WCRB2014

Insulin and branched-chain amino acid depletion during mouse in vitro preimplantation development alters postnatal growth and cardiovascular physiology

Velazquez Miguel Abraham , Sheth Bhav , Marfy-Smith Stephanie , Eckert Judith , Fleming Tom

Introduction: Maternal depletion of insulin in serum and branched-chain amino acids (BCAA) in uterine luminal fluid occurs in response to dietary protein undernutrition during the periconceptional period which leads to adverse offspring growth and cardiovascular phenotype (e.g. high blood pressure). This study aimed to determine whether insulin and/or BCAA depletion might be inductive in vivo factors for postnatal phenotype using an in vitro embryo culture mo...

ra0001p196 | (1) | WCRB2014

Effects of early embryo environment on embryonic stem cell derivation and behaviour

Cox Andrew L , Sun Congshan , Sheth Bhav , Fleming Tom , Smyth Neil

The embryonic environment can induce permanent changes in metabolism and increase disease risk in adults. Rodent models show that modifications in maternal diet exclusively during pre-implantation development can induce metabolic disease in adults. Changes must therefore occur within the early embryo and be maintained throughout development. Determining adaptive mechanisms is challenging due to the size of the early embryo. We generated ES cells from inbred C57BL/6 mice as a m...

ra0002o014 | SRF Student Prize Session | SRF2015

Maternal protein restriction around conception reduces the neural stem cells during mouse fetal brain development and alters neuronal differentiation during gestation

Gould Joanna Mary , Fleming Tom , Airey Chris J , Marfy-Smith Stephanie Jane , Willaime-Morawek Sandrine

Maternal malnutrition during pregnancy is detrimental to fetal development and increases the risk of many chronic diseases in later life i.e. neurological consequences such as increased risk of schizophrenia. Previous studies have shown maternal protein malnutrition during pregnancy and lactation compromises brain development in late gestation and after birth, affecting structural, biochemical, and pathway dynamics with lasting consequences for motor and cognitive function. Ho...

ra0003o002 | Oral Communications 1: Ovary | SRF2016

The effect of folic acid supplementation during the juvenile pubertal period or adulthood on gene expression in the ovary

Escarate Reyna Penailillo , Burton Mark , Burdge Graham , Eckert Judith , Fleming Tom , Lillycrop Karen

Introduction: Women with mutations in BRCA1 gene have an increased lifetime risk of ovarian cancer. Many studies but not all have shown that an adequate folate intake is protective against many cancers including ovarian, but recent studies have shown that high levels of folic acid (FA) supplementation can promote cancer risk. The aim of this study is to determine FA impact on the expression of cancer related genes in the ovary such as OCT4 a pluripotency factor, BRCA1 related ...