Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0003o020 | SRF Student Prize Session | SRF2016

Maternal high fat diet (HFD) in the adult offspring brain modifies cell density and neuronal density

Pedraza Diego Ojeda , Jane-Coupe Kate , Earl Megan , Hutton Oliver , Eckert Judith , Willaime-Morawek Sandrine

Introduction: An upward trend in maternal obesity is rising every year. Different data suggest that maternal obesity during gestation may have effects on a high risk of children of developing physiological and psychological dysfunctions in later life. Animal models suggest that a maternal high fat diet (HFD) during pregnancy could have enduring consequences on brain structure and development in the offspring. Our aim is to evaluate the effects of maternal HFD on both offspring...

ra0001p161 | (1) | WCRB2014

Differential role of TGFβ2 during follicle activation and preantral follicle growth

Oliver Elizabeth , Laird Mhairi , Franks Stephen , Hardy Kate

Introduction: Female mammals are born with a finite reserve of primordial follicles which dictates the reproductive lifespan. Despite this the factors responsible for primordial follicle activation remain largely unknown. The transforming growth factor beta (TGFβ) superfamily has been implicated in early follicle development however little attention has been given to the effects of TGFβ2, expression of which has been demonstrated in the ovary. This study aimed to exa...

ra0002o012 | Oral Communications 2: Ovarian function | SRF2015

Differential effects of transforming growth factor beta 2 in two different models of preantral follicle growth

Oliver Elizabeth , Laird Mhairi , Franks Stephen , Hardy Kate

The onset of mammalian follicle development is marked by granulosa cell (GC) proliferation, GC shape change, and oocyte growth. Despite their critical role in female fertility the factors and mechanisms underlying these cellular changes remain largely unknown. The transforming growth factor beta (TGFβ) superfamily has been strongly implicated in early follicle growth. Disruption of the function or expression of members of the TGFβ superfamily, receptors or key compon...

ra0003o033 | Oral Communications 4: Early Development 2 | SRF2016

Spermbots: Magnetic microrobots that assist sperm cells on their journey, opening new routes to assisted reproduction

Schwarz Lukas , Medina-Sanchez Mariana , Magdanz Veronika , Schmidt Oliver G.

Introduction: The interdisciplinary field of microrobotics recently sparked interest especially in potential applications of mobile microscale devices that can operate remotely-controlled inside the human body. This study presents an approach towards such an application, namely assisted reproduction with the help of tiny sperm carriers, so-called spermbots. Spermbots are synthetic tubes or helices that couple to single sperm cells and assist their movement by providing guidanc...

ra0003o024 | Oral Communications 3: Environmental and programming effects on reproduction | SRF2016

Maternal protein restriction around conception alters the foetal mouse brain by reducing the neural stem cells and increasing neuronal differentiation during gestation, which might be associated with the adult behavioural deficits

Gould Joanna , Pearson-Farr Jenny , Airey Chris , Semmence Oliver , Gould Philippa , Willaime-Morawek Sandrine , Fleming Tom

Introduction: Maternal malnutrition during pregnancy is detrimental to fetal development and increases the risk of many chronic diseases in later life i.e. neurological consequences such as increased risk of schizophrenia. Previous studies have shown maternal protein malnutrition during pregnancy and lactation compromises brain development in late gestation and after birth, affecting structural, biochemical and pathway dynamics with lasting consequences for motor and cognitive...

ra0002p010 | (1) | SRF2015

Characterisation of the dog as a sentinel species for human exposure to endocrine disrupting chemicals

Sumner Rebecca , Byers Andrew , Taylor Oliver , Moxon Rachel , White Natasha , Craigon Jim , Zhang Zulin L , England1 Gary C W , Lea Richard

Introduction: A temporal decline in canine male fertility parallels that reported in the human. Our hypothesis is that this is associated with exposure to environmental chemicals (ECs). We investigated i) a relationship between canine testicular chemical profiles and Sertoli cell numbers and ii) the effects of testicular concentrations of chemicals on sperm DNA fragmentation, motility and vitality.Methods: Canine adult testes (routine castrations) from t...

ra0002p029 | (1) | SRF2015

Successful isolation, culture and karyotyping of equine placental cells from failed early pregnancies

Rose Belinda Vivienne , Cabrera-Sharp Victoria , Cameron Ian , Crabtree James , Crowhurst James , Firth Marvin , Ghosh Sharmila , McGladdery Andrew , Neal Huw , Pynn Jan , Pynn Oliver , Raudsepp Terje , Smith Charlie , Verheyen Kristien , Wathes D Claire , Wise Zara , de Mestre Amanda

Early pregnancy loss (EPL) in the mare is defined as loss of pregnancy between initial detection and day 65 of gestation. It occurs in 7–10% of pregnancies and yet little is known about the underlying pathologies. A lack of suitable conceptus material has limited investigation into the role of chromosomal defects in EPL. The objective of this study was to develop a method to isolate and culture placental cells isolated from failed pregnancies to enable further genetic cha...

ra0001p076 | (1) | WCRB2014

Are cumulus cells additional players in calcium signalling during cattle oocyte fertilisation?

Sutton-McDowall Melanie L , Thompson Jeremy G

Upon sperm entry within the oocyte, pulsatile calcium release occurs, facilitating activation of the oocyte. While calcium signalling has been characterised in mouse oocytes, patterns within the whole cumulus oocyte complex (COC) and in larger mammals such as cow, are yet to be determined. The aim of this study was to investigate calcium changes in media, cumulus and oocytes during in vitro fertilisation in cattle oocytes.Cattle COCs were insemi...

ra0002s013 | Symposia 3: Management of livestock fertility | SRF2015

Embryo-uterine dialogue in the cow

Sandra Olivier

Successful pregnancy depends on a succession of complex biological processes that are regulated temporally and spatially. In term of contribution to pregnancy, the mother not only produces oocytes but she also hosts the whole gestation, mainly in the uterus. For decades, a major focus has been made on oocyte and embryo quality in term of contribution to progression and issue of pregnancy in mammals, including cattle. Nevertheless, in the bovine species, recent studies have cle...

ra0001p043 | (1) | WCRB2014

Effect of cAMP regulators on bovine cumulus–oocyte communication and embryo development in vitro

Nogueira Marcelo G , Machado Mariana F , Li Haijun , Razza Eduardo M , Gilchrist Robert B , Sutton-McDowall Melanie L , Thompson Jeremy G

Introduction: Preventing spontaneous maturation in vitro by adding cAMP regulators is thought to maintain the communication between oocyte and cumulus cells via gap junction communication (GJC), hence promoting developmental competence. We aimed to assess the integrity of gap junction maintenance in cumulus oocyte complexes (COCs) after pre-maturation with IBMX and Forskolin (FSK) and the influence on bovine embryo development.Materials...