Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0002p048 | (1) | SRF2015

The effect of prenatal exposure to androgen and to a high fat diet on obesity in female mice

Laird Mhairi , Hardy Kate , Franks Stephen

Polycystic ovary syndrome (PCOS) is a complex endocrine disorder that presents in women of reproductive age. The aetiology of PCOS is poorly understood, however it is likely that women with this disorder are predisposed to produce excess androgen at or well before puberty. Environmental and lifestyle factors then contribute to the multifarious symptoms observed. One such key external factor is diet. These studies utilised a prenatally exposed androgen mouse model to investigat...

ra0001p160 | (1) | WCRB2014

Androgen action within the preantral follicle; a role for the epidermal growth factor receptor?

Thomson Kacie , Laird Mhairi , Franks Stephen , Hardy Kate

Introduction: Polycystic ovary syndrome (PCOS) is the most common cause of anovulatory infertility affecting over 5% of the female population. PCOS is characterized by aberrant early follicle development in which hyperandrogenism is thought to play a key role. However, the molecular mechanisms of androgen action within the ovary remain largely unknown. Recent evidence suggests androgens may be acting, in part, through modulation of growth factor signalling. We have recently sh...

ra0001p161 | (1) | WCRB2014

Differential role of TGFβ2 during follicle activation and preantral follicle growth

Oliver Elizabeth , Laird Mhairi , Franks Stephen , Hardy Kate

Introduction: Female mammals are born with a finite reserve of primordial follicles which dictates the reproductive lifespan. Despite this the factors responsible for primordial follicle activation remain largely unknown. The transforming growth factor beta (TGFβ) superfamily has been implicated in early follicle development however little attention has been given to the effects of TGFβ2, expression of which has been demonstrated in the ovary. This study aimed to exa...

ra0001p205 | (1) | WCRB2014

The impact of oxidative stress on chaperone-mediated human sperm–egg interaction

Grace Bromfield Elizabeth , Nixon Brett , Redgrove Kate , Aitken Robert John

Introduction: Defective sperm–egg recognition is recognised as one of the major causes of failed fertilization in IVF programs. Our recent findings have revealed that such defects may be linked to the molecular chaperone, heat shock protein A2 (HSPA2), due to its ability to facilitate the formation of multimeric zona pellucida (ZP)-receptor complexes on the surface of human spermatozoa. This study aimed to examine the impact of oxidative stress on the formation of these Z...

ra0002o001 | SRF Post Doctoral Prize Session | SRF2015

Hyperandrogenism modulates adipokine gene expression in mouse adipocytes: implications for PCOS

Lerner Avi , Kewada Drashti , Okolo Anthony , Hardy Kate , Franks Stephen

Polycystic ovary syndrome (PCOS) is a common endocrinopathy that is associated with anovulatory infertility, menstrual disturbances as well as an adverse metabolic profile. Hyperandrogenism is the hallmark of PCOS and originates predominantly from ovarian theca cells. Obesity increases androgen synthesis, partly due to accompanying hyperinsulinemia but also as a result of an effect of adipokines on ovarian steroidogenesis (Comim et al. PLoS ONE 8</str...

ra0002o012 | Oral Communications 2: Ovarian function | SRF2015

Differential effects of transforming growth factor beta 2 in two different models of preantral follicle growth

Oliver Elizabeth , Laird Mhairi , Franks Stephen , Hardy Kate

The onset of mammalian follicle development is marked by granulosa cell (GC) proliferation, GC shape change, and oocyte growth. Despite their critical role in female fertility the factors and mechanisms underlying these cellular changes remain largely unknown. The transforming growth factor beta (TGFβ) superfamily has been strongly implicated in early follicle growth. Disruption of the function or expression of members of the TGFβ superfamily, receptors or key compon...

ra0002o028 | Oral Communications 4: Female reproduction | SRF2015

Regulation of the prostaglandin synthase COX2 by epidermal growth factor requires steroid receptor coactivator interacting protein in human myometrium

Hudson Claire A , Heesom Kate J , Bernal Andres Lopez

Human parturition at term and preterm involves inflammatory and non-inflammatory pathways and includes activation of the intrauterine prostaglandin (PG) cascade, resulting in synchronised membrane rupture, cervical dilation, and myometrial contractility. A key mediator of uterine PG production is the highly inducible enzyme cyclooxygenase 2 (COX2 or PTGS2). We have identified steroid receptor coactivator interacting protein (SIP or KANK2) to be highly expressed in human myomet...

ra0001s005 | Testis biology and spermatogenesis | WCRB2014

The contributions of importin proteins to spermatogenesis: roles in signalling, gene transcription and stress responses

Miyamoto Yoichi , Whiley Penny , Young Julia , Arjomand Arash , Yoneda Yoshihiro , Jans David A , Loveland Kate L

Importin protein family members are best known for serving critical roles in mediating regulated nucleocytoplasmic transport, and many are essential to developmental events including gametogenesis. We proposed that coordinated expression of an importin and a cell-specific transcription factor would drive a developmental switch by enabling transcription and other nuclear factors to enter the nucleus appropriately. Analyses of importin alpha (IMPα) and beta (IMPβ) mRNA...

ra0001p211 | (1) | WCRB2014

The role of the molecular chaperone HSPA2 in unexplained failure of IVF

Nixon Brett , Bromfield Elizabeth , Redgrove Kate , Baker Mark A , Aitken Robert John

Introduction: A common defect encountered in the spermatozoa of male infertility patients is an idiopathic failure of sperm–egg recognition. The aim of this study was to investigate the molecular basis of this condition.Materials and methods: For the purpose of this study we compared the proteomic profiles of spermatozoa from male infertility patients exhibiting an impaired capacity for sperm–egg recognition with that of sperm from males of pro...

ra0001p220 | (1) | WCRB2014

Crosstalk of TGFβ superfamily with Smad1 in testis

Chan Kai-Hui , Galuska Sebastian P , Loveland Kate L , Lutz Konrad

The TGFβ superfamily consists of more than 30 ligands, which exert their multiple functions during early embryonic development and in adult tissues. During spermatogenesis, several TGFβ superfamily ligands are present simultaneously to regulate critical stages of development through shared receptors and overlapping transduction molecules. However, the understanding of this promiscuity of signalling pathways remains unfortunately incomplete. Our aim is to study the me...