Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p013 | Testis | WCRB2014

Exogenous androgens reduce the expression of INSL3, a hormone involved in normal testicular descent, in fetal Leydig cells

Duncan W Colin , Connolly Fiona , Boswell Lyndsey , Burt Graeme , McNeilly Alan S , Rae Mick

Introduction: Hypothalamo–pituitary–testicular feedback is intact in the ovine fetus. Exogenous testosterone suppresses fetal LH and testicular steroidogenesis to maintain normal circulating testosterone concentrations. INSL3 is a non-steroidal hormone secreted by the fetal testis whose roles include facilitating testicular descent. We hypothesised that the homeostatic response to increased androgens would have a detrimental effect on fetal INSL3 expression....

ra0002o019 | SRF Student Prize Session | SRF2015

Obesity in PCOS: a consequence of prenatally programmed reduced energy expenditure

Siemienowicz Katarzyna Joanna , Rae Mick , Lerner Avi , Franks Stephen , Duncan W Colin

Polycystic ovary syndrome, a common endocrine condition affecting up to 10% of women of reproductive age, is associated with an increased risk of developing insulin resistance and obesity. Obesity is associated with increased energy intake or reduced energy expenditure. In this context, postprandial thermogenesis (PPT), is an important constituent of energy expenditure.Our lab utilizes a clinically realistic ovine model of PCOS, where pregnant Scottish G...

ra0002p031 | (1) | SRF2015

Does maternal progesterone supplementation in early pregnancy affect fetal development?

Mareckova Magda , Siemienowicz Katarzyna Joanna , Wang Yili , Rae Mick , Duncan W Colin

Some adult diseases are programmed in utero by fetal exposure to abnormal concentrations of steroid hormones. Threatened miscarriage in early pregnancy is treated by progesterone in many countries although robust evidence of efficacy is lacking. We hypothesised that increased progesterone concentrations may alter fetal development. In a small pilot study, using a pregnant sheep model, we administered 200 mg progesterone twice weekly from d20–d75 of gestation and ...

ra0002p047 | (1) | SRF2015

Waddlia chondrophila stimulates CXCL8 expression in ruminant trophoblast cells via p38 and p42/44 MAPK dependent pathways

Wheelhouse Nick M , Storrie Skye , Vacca Francesco , Barlow Peter , Rae Mick , Longbottom David

Background: Waddlia chondrophila (W. chondrophila) is an emerging abortifacient pathogen which has been identified in the placentae of humans and cattle. The organism is a member of the order Chlamydiales, and shares many similarities at the genome level, and in growth studies, with other well-characterised zoonotic chlamydial abortifacients, such as Chlamydia abortus (C. abortus). We have previously observed significant responses i...

ra0003o016 | SRF Student Prize Session | SRF2016

Exaggerated metabolic changes during puberty precede adult obesity and hyperlipidemia in an ovine model of Polycystic Ovary Syndrome (PCOS)

Siemienowicz Katarzyna , Coukan Flavien , Lerner Avigdor , Franks Stephen , Rae Mick , Duncan Colin

Women with Polycystic Ovary Syndrome are at increased risk of developing insulin resistance, obesity and dyslipidemia. Amplified metabolic perturbations during puberty may be a central factor contributing to metabolic phenotype of adult PCOS. Using a clinically realistic ovine model of PCOS we reported hyperinsulinaemia and early fatty liver changes, with no difference in body weight and adiposity, in adolescence.Here we aimed to further examine metaboli...

ra0001p321 | (1) | WCRB2014

Fetal overexposure to androgens, not estrogens, leaves a permanent legacy in terms of adrenal function in offspring

Ramaswamy Seshadri , Grace Cathal , Howie Forbes , Connolly Fiona , Konovalova Julia , McNeilly Alan S , Duncan W Colin , Rae Mick

Introduction: Overexposure to androgens during fetal life creates offspring with a polycystic ovary syndrome (PCOS)-like phenotype. Whether this phenotype is attributable to androgens during fetal life, or estrogens via maternal/placental metabolism, remains uncertain. The potential for in utero steroidal disruption of adrenal development and function is also unclear. We have examined the adrenal during development and adulthood in response to in utero androg...

ra0003o026 | Oral Communications 3: Environmental and programming effects on reproduction | SRF2016

Fetal androgens determine adult pancreatic function

Rae Mick , Ramaswamy Seshadri , Grace Cathal , Mattei Ashley , Siemienowicz Kasia , Brownlee Bill , MacCallum Janis , McNeilly Alan , Duncan Colin

Introduction: Maternal androgen excess in pregnant sheep programmes a PCOS-like phenotype in female offspring. We previously demonstrated a hyperinsulinaemic offspring phenotype in response to maternal androgen excess, but such regimens also increase estradiol concentrations, and may effect adrenal steroidogenesis, hence the role of androgens remains unclear.Materials and Methods: To examine contributions of different steroid classes, and to determine if...

ra0001p073 | (1) | WCRB2014

Early development of bovine embryos depends on the cooperative action between oxidative and endoplasmic reticulum stresses

Yoon Seung-Bin , Yang Hae-Jun , Mun Seong-Eun , Jeong Pil-Soo , Choi Seon-A , Park Young-Ho , Song Bong-Seok , Sim Bo-Woong , Kim Ji-Su , Kim Sun-Uk , Chang Kyu-Tae , Lee Sang-Rae

The coupling of reactive oxygen species (ROS) and endoplasmic reticulum (ER) stress has been explored using a variety of biological systems, but little is known regarding their role in the early development of mammalian embryos. Here, we demonstrated that early embryonic development of in vitro-produced (IVP) bovine embryos was governed by the cooperative action between ROS and ER stress. Compared to 5% O2 tension, 20% O2 significantly decreased ...