Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0002o019 | SRF Student Prize Session | SRF2015

Obesity in PCOS: a consequence of prenatally programmed reduced energy expenditure

Siemienowicz Katarzyna Joanna , Rae Mick , Lerner Avi , Franks Stephen , Duncan W Colin

Polycystic ovary syndrome, a common endocrine condition affecting up to 10% of women of reproductive age, is associated with an increased risk of developing insulin resistance and obesity. Obesity is associated with increased energy intake or reduced energy expenditure. In this context, postprandial thermogenesis (PPT), is an important constituent of energy expenditure.Our lab utilizes a clinically realistic ovine model of PCOS, where pregnant Scottish G...

ra0002p031 | (1) | SRF2015

Does maternal progesterone supplementation in early pregnancy affect fetal development?

Mareckova Magda , Siemienowicz Katarzyna Joanna , Wang Yili , Rae Mick , Duncan W Colin

Some adult diseases are programmed in utero by fetal exposure to abnormal concentrations of steroid hormones. Threatened miscarriage in early pregnancy is treated by progesterone in many countries although robust evidence of efficacy is lacking. We hypothesised that increased progesterone concentrations may alter fetal development. In a small pilot study, using a pregnant sheep model, we administered 200 mg progesterone twice weekly from d20–d75 of gestation and ...

ra0003o016 | SRF Student Prize Session | SRF2016

Exaggerated metabolic changes during puberty precede adult obesity and hyperlipidemia in an ovine model of Polycystic Ovary Syndrome (PCOS)

Siemienowicz Katarzyna , Coukan Flavien , Lerner Avigdor , Franks Stephen , Rae Mick , Duncan Colin

Women with Polycystic Ovary Syndrome are at increased risk of developing insulin resistance, obesity and dyslipidemia. Amplified metabolic perturbations during puberty may be a central factor contributing to metabolic phenotype of adult PCOS. Using a clinically realistic ovine model of PCOS we reported hyperinsulinaemia and early fatty liver changes, with no difference in body weight and adiposity, in adolescence.Here we aimed to further examine metaboli...

ra0003o026 | Oral Communications 3: Environmental and programming effects on reproduction | SRF2016

Fetal androgens determine adult pancreatic function

Rae Mick , Ramaswamy Seshadri , Grace Cathal , Mattei Ashley , Siemienowicz Kasia , Brownlee Bill , MacCallum Janis , McNeilly Alan , Duncan Colin

Introduction: Maternal androgen excess in pregnant sheep programmes a PCOS-like phenotype in female offspring. We previously demonstrated a hyperinsulinaemic offspring phenotype in response to maternal androgen excess, but such regimens also increase estradiol concentrations, and may effect adrenal steroidogenesis, hence the role of androgens remains unclear.Materials and Methods: To examine contributions of different steroid classes, and to determine if...

ra0002p009 | (1) | SRF2015

A model of tissue-engineered (3d) decidua to study the effects of environmental pollutants on endometrial physiology

Mannelli Chiara , Szostek Anna , Letta Francesca , Łukasik Karolina , Jankowska Katarzyna , Piotrowska-Tomala Katarzyna , Paulesu Luana Ricci , Skarzynski Dariusz Jan

The increasing exposure to environmental chemicals is a burden for human reproduction, and can alter endometrial functions and maternal–embryo interactions. The available in vitro endometrial models often fail to represent the complexity of cellular environment, cells shape and organization, and could give misleading results on the effects of environmental chemicals. Here, a tissue-engineered (3D) decidua was used as a toxicological model. Human stromal cells wer...

ra0001p116 | (1) | WCRB2014

Expression and function of lysophosphatidic acid in theca cells of the bovine ovarian follicle

Sinderewicz Emilia , Boruszewska Dorota , Kowalczyk-Zieba Ilona , Staszkiewicz Joanna , Grycmacher Katarzyna , Woclawek-Potocka Izabela

In cow, lysophosphatidic acid (LPA) stimulated synthesis and secretion of luteotropic factors in ovary, which affected growth and development of ovarian follicles. Theca cells, among other components of ovarian follicle, play an essential role in ovulation, which depends on intrafollicular prostaglandin synthesis. In bovine reproductive tract, modulation of prostaglandin synthesis occurs under influence of LPA. Thus, the aim of this study was to determine possible correlation ...

ra0001p117 | (1) | WCRB2014

Expression and function of lysophosphatidic acid in granulosa cells of the bovine ovarian follicle

Woclawek-Potocka Izabela , Sinderewicz Emilia , Boruszewska Dorota , Kowalczyk-Zieba Ilona , Staszkiewicz Joanna , Grycmacher Katarzyna

Mural granulosa cells participate in development and differentiation of ovarian follicle. In bovine reproductive system, LPA is involved in autocrine and paracrine signaling, through LPA receptors (LPAR)s. We demonstrated before that LPA modulated prostaglandin (PG) synthesis in uterus and ovary and stimulated estradiol (E2) synthesis in granulosa cells in bovine follicle. As the ovarian follicle development and differentiation leading to oocyte ovulation depend on ...

ra0001p221 | (1) | WCRB2014

Meiotic and post-meiotic germ cells up-regulate blood–testis barrier function

Olcorn Justine Samantha , Rainczuk Katarzyna Eliza , Foo Caroline , Haverfield Jenna T , Stanton Peter G

Introduction: The blood–testis barrier (BTB) remains semi-permeable to tracers of increasing molecular weight in an adult rat model of spermatogenic re-initiation. Complete closure of the BTB occurred when steps 2–7 round spermatids re-appeared in the epithelium, and coincided with the localisation of a new tight junction (TJ) protein claudin-12 (Cldn12) at the BTB. We hypothesise that meiotic and/or post-meiotic germ cells up-regulate Sertoli cell TJs, and aim to de...

ra0003o030 | Oral Communications 4: Early Development 2 | SRF2016

Different mechanism of lipid accumulation in embryoblast and trophoblast cells trigger by lipid excess

Schindler Maria , Pendzialek Mareike , Grybel Katarzyna , Guerke Jacqueline , Seeling Tom , Fischer Bernd , Santos Anne Navarrete

Introduction: Differentiation of the embryoblast and trophoblast cell lineages is an ontogenetic milestone in preimplantation embryo development. Both cell lineages are supplied with nutrients from uterine secretions, reflecting an ultrafiltrate of maternal plasma. We have recently shown that an insulin-dependent diabetes mellitus leads to maternal hyperlipidaemia and to a strong increase in intracellular lipids in trophoblast and, especially, embryoblast cells in rabbit blast...

ra0001p276 | (1) | WCRB2014

Glucocorticoids trigger macrophage migration inhibitory factor (MIF) secretion by decidualized human endometrial stromal cells in vitro: the modulatory effect of Bisphenol A

Mannelli Chiara , Szostek Anna , Ciaffarafa Sara , Carotenuto Claudiopietro , Ietta Francesca , Romagnoli Roberta , Piotrowska-Tomala Katarzyna , Paulesu Luana , Skarzynski Dariusz

Introduction: Pro-inflammatory cytokines are important mediators of the early phases of gestation. Among all these cytokines, the macrophage migration inhibitory factor (MIF) plays significant roles in pregnancy establishment. In many cell types, low concentrations of glucocorticoids (GCs) are known to enhance MIF secretion, but the effect of GCs on endometrial cells has not been elucidated. Bisphenol A (BPA) is an environmental chemical that can alter reproductive physiology ...