Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p197 | (1) | WCRB2014

Trophectoderm stem cells to model the effect of altered periconceptional diet on embryos

Chang Yi-Lung , Fleming Tom , Smyth Neil

Introduction: Poor maternal diet during development induces physiological, metabolic and anatomical alterations in offspring. These may increase immediate survival chances, but can enhance the risk of disease in later life. Maternal low protein diet (LPD) exclusively in pre-implantation development induces changes in endocytosis, postnatal hypertension, cardiovascular dysfunction and alters behaviour in our mouse model. We have previously shown changes in the embryonic stem ce...

ra0003o017 | SRF Student Prize Session | SRF2016

Derivation and use of mouse embryonic stem cell lines as model for mechanistic analysis of periconceptional developmental programming

Khurana Pooja , Cox Andy , Fleming Tom , Smyth Neil

Introduction: The developmental origins of health and disease (DOHaD) concept proposes that maternal environment during pregnancy may influence offspring health and predispose to chronic disease risk in later life. Our previous mouse in vivo studies showed that maternal low protein diet (LPD) and advanced maternal age (AMA) programme the preimplantation embryo to adult disease, notably cardiometabolic dysfunction. To test this hypothesis and understand the mechanistic...

ra0001p095 | (1) | WCRB2014

The effect of vitrification on subsequent mouse embryo development and postnatal health

Khalif Raja Ili Airina Raja , Smyth Neil , Fleming Tom , Velazquez Miguel Abraham , Sheth Bhav , Smith Stephanie

Short-term effects of cryopreservation on embryo development, survival and after embryo transfer (ET), resulting pregnancy rate have been documented. However, long-term effects on offspring remain relatively unexplored.Materials and methods: Using a mouse model, we investigated the effect of vitrification on blastocyst trophectoderm (TE) and inner cell mass (ICM) cell numbers by differential staining and postnatal growth and cardiovascular and metabolic ...

ra0001p110 | (1) | WCRB2014

Epigenetic regulation of Gata-6 transcription factor expression in mouse primitive endoderm extra-embryonic lineage induced by maternal protein restricted diet

Sun Congshan , Sheth Bhav , Smyth Neil , Cox Andrew L. , Denisenko Oleg , Fleming Tom

Introduction: Maternal low protein diet exclusively during preimplantation mouse development (Emb-LPD) induces compensatory changes in extra-embryonic lineages including primitive endoderm (PE) to enhance nutrient delivery during later gestation, for example, stimulation of endocytosis. Whilst such responses protect fetal growth, adult offspring exhibit increased cardiometabolic disease risk. Here, we investigate epigenetic mechanisms underlying maternal dietary programming, u...

ra0001p196 | (1) | WCRB2014

Effects of early embryo environment on embryonic stem cell derivation and behaviour

Cox Andrew L , Sun Congshan , Sheth Bhav , Fleming Tom , Smyth Neil

The embryonic environment can induce permanent changes in metabolism and increase disease risk in adults. Rodent models show that modifications in maternal diet exclusively during pre-implantation development can induce metabolic disease in adults. Changes must therefore occur within the early embryo and be maintained throughout development. Determining adaptive mechanisms is challenging due to the size of the early embryo. We generated ES cells from inbred C57BL/6 mice as a m...

ra0002o008 | Oral Communications 1: Embryo and Implantation | SRF2015

The effect of IVF and embryo culture on mouse development and adult health

Aljahdali Anan , Raja Khalif Raja Ili Airina , Velazquez Miguel Abraham , Sheth Bhav , Smyth Neil , Fleming Tom

Several million babies have been born worldwide since IVF and assisted-reproductive technologies (ART) became available. However, reports link IVF techniques with adverse short- and long-term health outcomes.1,2,3 Using a mouse model, we investigated the effect of IVF on the total cell number in blastocyst stage embryos and the postnatal health of offspring.Methods: Experimental groups; (NM) C57/BL6 non-superovulated females naturally mated wi...

ra0003p060 | (1) | SRF2016

Effect of in vitro fertilization (IVF) and embryo culture duration on mouse development and postnatal health

Aljahdali Anan , Khalif Ili Raja , Velazquez Miguel A , Sheth Bhav , Smyth Neil R , Fleming Tom

Since the advent of IVF (in vitro fertilisation), several million babies have been born worldwide. However, reports link in vitro techniques with adverse short and long-term health outcomes. Using a mouse model, we investigated the effect of IVF and duration of culture on blastocyst development and cell number and the postnatal health of offspring. Experimental groups (8–13 litters each): NM (natural mating, non-superovulated) IV-ET-2Cell (2-cell embryos...

ra0002p051 | (1) | SRF2015

SPRASA knockout mice are sub-fertile

Prendergast Deborah , Liu Bonnia , Sheling Andrew Neil , Chamley Larry

Introduction: SPRASA is a protein, that is expressed in the acrosome of sperm, as wel as on the oolemma of many mammals, which we identified as the target of antisperm antibodies from some infertile men. In order to further understand the importance of SPRASA we produced knockout mice and examined the effect of this gene knockout on murine fertility.Methods: With ethics approval (AEC_R811) knockout mice were generated lacking the expression of exons 4 an...

ra0002p041 | (1) | SRF2015

Regulation of CyclinD2 by Smad3 and Foxl2 during early follicle development

Granados-Aparici Sofia , Sharum Isam , Hardy Kate , Franks Stephen , Waite Sarah , Chapman Neil , Fenwick Mark

Introduction: Primordial follicles are relatively quiescent structures that form the basis of the ovarian reserve. Maintenance of the quiescent state, and conversely, release from this state towards irreversible growth, involves mechanisms that are currently unresolved. Two transcription factors have been implicated alongside this process in granulosa cells (GCs). Specifically, Smad3 was recently identified in the nuclei of GCs in small single-layered follicles whereas Foxl2 i...