Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0003o018 | SRF Student Prize Session | SRF2016

FACS-sorted putative oogonial stem cells from the mouse ovary are neither DDX4-positive nor germ cells

Zarate-Garcia Larissa , Lane Simon I. R. , Merriman Julie A. , Jones Keith T.

Whether the adult mammalian ovary contains oogonial stem cells (OSCs) is controversial. They have been isolated by fluorescence-activated cell sorting (FACS) using the germline marker DEAD box polypeptide 4 (DDX4), previously assumed to be cytoplasmic, not surface-bound. Furthermore their stem cell and germ cell characteristics remain disputed. We applied a validated protocol to isolate mouse putative OSCs from whole ovarian cell suspensions, then assessed their in vitro</...