Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0002p030 | (1) | SRF2015

The endocrine disrupting chemicals bisphenol A, dichlorodiphenyltrichloroethane (DDT), methoxyclor and ethinylestradiol modulate thecal steroidogenesis in vitro

Cheewasopit Warakorn , Knight Phil G

Introduction: Previous reports in different species have shown that various endocrine disrupting chemicals (EDCs) can modulate ovarian steroidogenesis with the majority of studies focussing on granulosa cells. To test the hypothesis that exposure to EDCs might also perturb thecal steroidogenesis we conducted in vitro dose–response experiments to evaluate the direct effects of selected EDCs on androstenedione and progesterone production by cultured bovine theca ce...

ra0002p004 | (1) | SRF2015

Effect of macrophages on steroidogenesis and cell migration in bovine ovaries

Samir Moafaq S , Mattar Dareen S , Knight Phil G

Introduction: Cyclic ovarian function involves continual tissue remodelling. During ovulation macrophages invade the ovary and secret pro-inflammatory cytokines such us TNF- and IL6 that have local actions on ovarian cells. Here we investigated i) the effect of macrophages on secretion of oestradiol by granulosa cells (TC) and androstenedione by theca cells (TC) and ii) the effect of macrophages on TC and stroma cell (SC) migration was also assessed using an in vitro ...

ra0002p049 | (1) | SRF2015

Does kisspeptin exert a local modulatory effect on ovarian steroidogenesis?

Mattar Dareen S , Cheewasopit Warakorn , Samir Moafaq , Knight Phil G

Introduction: Kisspeptin, a neuropeptide secreted in the hypothalamus and encoded by the Kiss-1 gene, has a role in promoting the release of GnRH and LH in various species. However, the possibility that kisspeptin exerts additional ‘peripheral’ actions at the level of the bovine gonad has not been investigated. The current aims were to investigate whether: i) kiss-1 and its receptor (GPR54) are expressed in the bovine ovary; ii) kisspeptin or kisspeptin antagonist ca...