Searchable abstracts of presentations at key conferences on reproductive biology and medicine
Previous issue | Volume 3 | SRF2016

Society for Reproduction and Fertility Annual Conference 2016

Winchester, UK
11 Jul 2016 - 11 Jul 2016

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A retrospective review of outcomes following endometrial scratch procedure in IVF at Fertility Exeter

Manning Louisa , Koussidis Georgios , Joels Lisa

Introduction: Mechanical endometrial injury (i.e. endometrial scratch) in the cycle preceding ovarian stimulation for IVF has been proposed to improve implantation in women with unexplained recurrent implantation failure (RIF).During the implantation window, there is a cross-talk between the embryo and endometrium to allow attachment, adhesion and invasion of the embryo. Endometrial scratch (ES) is suggested to induce changes in the immune system in the ...

ra0003p003 | (1) | SRF2016

Bi-directional regulation of miR-125a-3p expression by mural and cumulus granulosa cells of mice pre-ovulatory follicles

Har-Paz Efrat , Grossman Hadas , Shalgi Ruth

Introduction: MicroRNAs, transferred between cells through gap-junctions and fluids, serving as inter-cellular signaling molecules. miR-125a-3p, which can be found in follicular-fluid, is expressed and down-regulated by hCG in mural-granulosa-cells. Our preliminary results show that similar effect of hCG in oocytes enables resumption of first-meiotic division. Cumulus-cells and oocytes maintain diverged relationship that includes bi-directional transfer of RNAs and proteins. B...

ra0003p004 | (1) | SRF2016

The function of Histone Variants in female infertility

Lin Chih-Jen

Histone variants can replace canonical histones in the nucleosome and modify chromatin structure and gene expression. The histone variant H3.3 preferentially associates with active chromatin and has been implicated in the regulation of a diverse range of developmental processes. We recently showed that maternal Hira, a chaperone for the histone variant H3.3, is required for mouse development past the zygote stage. Male pronucleus formation is inhibited upon deletion of Hira du...

ra0003p005 | (1) | SRF2016

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ra0003p006 | (1) | SRF2016

Does Neuromedin B exert a local modulatory effect on ovarian steroidogenesis or cell proliferation?

Mattar Dareen , Laird Mhairi , Samir Moafaq , Cheewasopit Warakorn , Knight Phil

Introduction: Neuromedin B (NMB), a highly conserved bombesin-related decapeptide originally isolated from porcine spinal cord, has various physiological effects including the regulation of exocrine and endocrine secretions. The current aims were to investigate whether: (1) NMB is expressed in the bovine ovary (2) NMB or NMB antagonist can modulate ovarian steroidogenesis or proliferation by cultured bovine theca (TC) and granulosa (GC) cells.Methods: GC...

ra0003p007 | (1) | SRF2016

Alterations in the vasculature of placental but not endometrial tissue associated with small porcine foetuses compared to their normal-sized littermates

Stenhouse Claire , Hogg Charis , Donadeu Xavier , Ashworth Cheryl

Introduction: Low piglet birth weight has severe consequences for neonatal and adult development that cannot be remedied post-natally. It is hypothesised that impaired foetal growth occurs due to inadequate placental vascularisation.Methods: Endometrial and placental tissues supplying small and normal-sized foetuses were collected from Large White x Landrace gilts at gestational days (GD) 30 (n=5), 60 (n=7) and 90 (n=5) for imm...

ra0003p008 | (1) | SRF2016

Generation of GFP transgenic cynomolgus monkeys

Seita Yasunari , Tsukiyama Tomonori , Iwatani Chizuru , Tsuchiya Hideaki , Matsushita Jun , Ema Masatsugu

Introduction: Nonhuman primates are considerered valuable human disease models. There are not many reports about transgenic monkey and especially no report about GFP cynomolgus monkey. Here, we report for the first time generation of whole body GFP expressing cynomolgus monkey.Methods: We constructed a lentiviral vector carries GFP under control CAG promoter. To generate a transgenic cynomolgus monkey expressed GFP uniformly, we compared injection timing...

ra0003p009 | (1) | SRF2016

Involvement of MyD88 in B-cell mediated immune response in a mouse model of LPS-induced fetal death

Plenagl Susanne , Busse Mandy , Nowak Desiree , Schumacher Anne , Zenclussen Ana Claudia

Introduction: During pregnancy, the maternal immune system has to tolerate the semi-allogenic fetus while protecting the mother and the unborn against pathogens. Subclinical infections that danger the fetus constitute a challenge for the medical system. A useful model to study mechanisms underlying infection-driven fetal death is a LPS-based mouse model. The recognition of LPS, a component of gram-negative bacteria membrane, leads to an immune answer that takes place through T...

ra0003p010 | (1) | SRF2016

Role of micro-RNAs in thrombospondin-1 expression in bovine luteal cells

Farberov Svetlana , Meidan Rina

Introduction: FGF2 and thrombospondin-1 (THBS1) expression in corpus luteum (CL) exhibited the most divergent profile of induction by prostaglandin F2a (PGF2α). FGF2, a potent angiogenic pro-survival factor was increased in the Day 4 CL. In contrast, the anti-angiogenic, apoptotic factors, THBS1, transforming growth factor beta 1 (TGFB1) and plasminogen activator inhibitor-1 (PAI-1) were upregulated specifically on Day 11, PGF2α-responsive CL. Functionally, THBS1 rev...

ra0003p011 | (1) | SRF2016

Effect of lipopolysaccharide on steroidogenesis and cell migration in the bovine ovary

Samir Moafaq , Mattar Dareen , Knight Phil

Introduction: Lipopolysaccharide (LPS) is a pathogen-associated molecular pattern (PAMP), expressed by gram-negative bacteria. The specific receptor for LPS on host cells is TLR4, one of 11 members of the TLR family involved in innate immunity. Here we investigated i) changes in GC/TC expression of TLR4 during follicle development, ii) The effect of LPS on oestradiol secretion from non-luteinised granulosa cells (GC) and androstendione from non-luteinised theca cells (TC), iii...

ra0003p012 | (1) | SRF2016

Investigation of the relationship between HA binding and sperm function tests including sperm DNA damage and chromatin maturity

Torabi Forough , Miller David

Introduction: During spermiogenesis, which is the last stage in spermatozoa maturation, a spermatozoa plasma membrane remodelling stage occurs which promotes the up-regulation of membrane receptors to aid the zona pellucida binding e.g. hyaluronic acid (HA) receptors.Researches have demonstrated that spermatozoa that are able to bind to HA in vitro are, in fact, more mature spermatozoa that have completed specific maturation processes and are ul...

ra0003p013 | (1) | SRF2016

Characterisation of extracellular vesicles produced by the Porcine oviductal epithelial cells using size exclusion chromatography

Jamaludin Nurul Akmal , Basatvat Shagayegh , Aboussahoud Wedad , Elliott Sarah , Hunt Stuart , Peacock Ben , Ebbens Steven , Fazeli Alireza

Introduction: The interaction of gametes and embryo with the maternal environment has a crucial impact on gametes maturation, embryonic development and subsequent pregnancy success. Recent studies have recognised extracellular vesicles (EVs) as a potent vehicles for intercellular communication. Defining the type of EVs which are produced by different reproductive cells will help us to understand how these structures can influence reproductive processes. The aim of the current ...

ra0003p014 | (1) | SRF2016

PI3K/AKT signaling pathway involvement in motility loss associated with prohibitin downregulation in sperm from infertile men

Chen Hong , Chai Ran Ran , Chen Guo Wu , O Wai Sum

Introduction: Phosphoinositide 3-kinase (PI3K) activity has been reported to be critical to sperm motility and mitochondrial ROS generation while mitochondrial membrane protein Prohibitin (PHB) controls PI3K/AKT pathway in somatic cells by regulating mitochondrial function. Our recent findings showed that sperm PHB expression is significantly decreased in infertile men with poor sperm quality by regulating mitochondrial morphology and function. The objective of this study is t...

ra0003p015 | (1) | SRF2016

Effect of atrazine on sperm mitochondrial function, acrosome reaction and fertilization competence: The bovine model

Komsky-Elbaz Alisa , Roth Zvi

Introduction: Atrazine (ATZ) is one of the most extensively used herbicides, known as a ubiquitous environmental contaminant and found in water sources. ATZ was detected in human amniotic fluid, serum and urine, however, the risk associated with ATZ exposure on sperm is less known.Methods: Sperm was isolated from fresh ejaculates or testicular-epididymis compartments (head, body, tail) and capacitated in vitro for 4 h with 0, 0.1, 1 or 10 μ...

ra0003p016 | (1) | SRF2016

Effect of pre-in vitro maturation using PACAP on nuclear and cytoplasmic maturation in porcine cumulus-oocyte complexes derived from small follicle

Park Kyu-Mi , Hyun Sang Hwan

For developmental competence of oocyte derived from small follicle (~3 mm in diameter SF), pre-IVM system was developed for in vitro culturing SF. The purpose of this study is to establish the optimal phase and concentration for exogenous addition of pituitary adenylate cyclase-activating peptide (PACAP) on pre-IVM. To establish the appropriate phase for pre-IVM, we assessed nuclear status according to culture duration. The result of the nuclear stage assessment of th...

ra0003p017 | (1) | SRF2016

Treatment of GDF8 during in vitro maturation increased phosphorylated SMAD2/3 and improved in vitro fertilized embryo developmental competence

Yoon Junchul David , Hyun Sang-Hwan

The purpose of this study is the effects of growth differentiation factor 8 (GDF8) and SB431542 (SB) on porcine oocytes in vitro maturation (IVM), subsequent embryonic development after in vitrofertilization (IVF). We were investigated the effect of GDF8 and SB treatment during IVM on nuclear maturation, intracellular glutathione (GSH), reactive oxygen species (ROS) levels, analyzed specific gene transcription and translation levels in cumulus cells after IVM, and emb...

ra0003p018 | (1) | SRF2016

Establish of a transgenic neural cell line with an astrocyte-specific inducible CreERT2 system

Hwang Seon-Ung , Yoon Junchul David , Han Yongquan , Eun Kiyoung , Kim Hyunggee , Hyun Sang-Hwan

Transgenic (TG) pigs are currently regarded as an important animal model for various biomedical researches including a disease modeling and a regenerative medicine. We tried to develop a pig astrocyte-specific CreERT2-LoxP recombination system as a part of TG pig model of brain tumor arisen from astrocytic cell lineage. We designed two vector systems one retains pig glial fibrillary acidic protein (GFAP) promoter-CreERT2 transgene and the other has GFP ge...

ra0003p019 | (1) | SRF2016

Effect of lysophosphatidic acid (LPA) on in vitro maturation of porcine oocytes and subsequent embryonic development after parthenogensis and in vitro fertilization

Kim Kyu-Jun , Jin Minghui , Hyun Sang-Hwan

Introduction: Lysophosphatidic acid (LPA) is a signaling molecule derived from phospholipid known to have biological activities such as stimulating proliferation and differentiation. The purpose of this study was to investigate the effect of LPA on IVM of porcine oocytes and subsequent embryonic development after IVF and parthenogenetic activation (PA).Methods: We examined nuclear maturation, intracellular GSH and ROS levels and subsequent embryonic deve...

ra0003p020 | (1) | SRF2016

Effects of the chemotherapy drugs cisplatin and doxorubicin on the follicles of the human ovary

Liu Jin , Lopes Federica , Morgan Stephanie , Nevin Lucy , Telfer Evelyn , Anderson Richard A , Spears Norah

Introduction: The effect of chemotherapy treatment on premenopausal women’s fertility has long been of concern. Here, we investigate the direct actions of chemotherapy drugs on the human ovary, examining effects of two commonly used drugs, cisplatin and doxorubicin.Method: Ovarian cortical tissue samples were collected from patients aged 27–34 who were undergoing elective caesarean section, with informed consent and Ethical Committee approval. ...

ra0003p021 | (1) | SRF2016

Impact of a contrasted metabolism on endometrial and peripheral signalling pathways at implantation in dairy cattle

Lesage-Padilla Audrey , Mauffre Vincent , Forde Niamh , Poiree Melanie , Giraud-Delville Corinne , Eozenou Caroline , Constant Fabienne , Lonergan Pat , Charpigny Gilles , Sandra Olivier

Intensive selection for milk production has led to a reduced reproduction efficiency of high-producing dairy cattle. The first month of pregnancy is associated to a high rate of pregnancy failures. In addition to embryo losses, pre-conceptional status of pregnant females has been reported to affect progeny performance after birth. This study aimed to investigate the impact of the conceptus on endometrium physiology and on peripheral blood leucocytes (PBL) gene expression patte...

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Analysis of follicle development in a mouse model with increased fertility

Stoddart Miranda , Ploutarchou Panayiota , Williams Suzannah

Introduction: The regulation of follicle development is not well understood, despite its importance in determining fertility, but there is evidence that the oocyte plays a key role. The female C1galt1 Mutant mouse has an oocyte-specific deletion of the T-synthase enzyme and as a result cannot synthesise core 1-derived O-glycans. Mutant females exhibit a phenotype of increased fertility and altered follicle development. To investigate the hypothesis that changes in the developm...

ra0003p024 | (1) | SRF2016

Therapeutic doses of phosphoramide mustard cause germ cell death in the prepubertal mouse testis

Rice Siobhan , Smart Ellie , Lopes Federica , Mitchell Rod , Spears Norah

The past few decades have seen marked improvements in life expectancy following childhood cancer due, in part, to advances in chemotherapy. While these drugs are effective in treating malignant disease, one of the main adverse outcomes can be infertility. This is of particular concern for prepubertal boys, for whom there are not yet any established methods of fertility preservation. The objective of this study was to gain a better understanding of the effects of the widely-use...

ra0003p025 | (1) | SRF2016

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ra0003p027 | (1) | SRF2016

Targeting lactate metabolism can be a novel therapeutic for the treatment of endometriosis

Ahmad Syed Furquan , Greaves Erin , Saunders Philippa , Horne Andrew

Introduction: Endometriosis is a chronic, hormone-dependent disorder characterized by the establishment and growth of endometrial tissue in extra-uterine sites, typically within the peritoneal cavity and causes debilitating pain. We have shown that peritoneal mesothelial cells recovered from the women with endometriosis have an altered energy metabolism with increased biosynthesis of lactate as a result of increased aerobic glycolysis. We hypothesize that ectopic endometrial t...

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Membrane potential contributes to generation of high amplitude [Ca2+]i oscillations and sperm behaviour in human spermatozoa

Nitao Elis , Publicover Stephen

Ca2+ signaling is crucial for regulation of sperm motility and [Ca2+]i oscillations may underlie ‘switching’ of sperm behaviors in human spermatozoa, which is believed to be important for sperm progression in the female tract. We have investigated the contribution of membrane potential (Vm) and the sperm Ca2+ channel CatSper to [Ca2+]i oscillations induced by progesterone (P4). Manipulation of Vm was p...

ra0003p030 | (1) | SRF2016

Investigating the role of the membrane receptor ADGRD1 in female fertility

Bianchi Enrica , Wright Gavin James

ADGRD1 is a member of the adhesion G protein-coupled receptors (aGPCRs), a family of membrane proteins that are categorised by the presence of a GAIN (GPCR autoproteolysis-inducing) domain between a large N-terminal extracellular region that is thought to bind signal-initiating ligands, and the signal-transducing G-protein-coupled seven transmembrane helices. The role and function of many aGPRCs is unclear and most of them are ‘orphan’ receptors, having no identified...

ra0003p031 | (1) | SRF2016

Effect of pHi on regulation of hyperactivated motility in human spermatozoa

Achikanu Cosmas , Publicover Stephen

CatSper channels are expressed specifically in the principal piece of the sperm flagellum. In human sperm Catsper channel activation is polymodal and sensitive to increased pHi, progesterone and other agonists, potentially resulting in synergistic enhancement of Ca2+ influx and hyperactivation (1) We have investigated if the interaction of pHi with the CatSper agonist progesterone and 4-aminopyridine (4AP), a potent activator of hyperactivation in human sperm. [Ca<s...

ra0003p032 | (1) | SRF2016

Gonadotropins induce expression of versican in porcine oocyte-cumulus extracellular matrix and mural granulosa cells

Nagyova Eva , Salustri Antonietta , Kalous Jaroslav , Kubelka Michal , Camaioni Antonella

Introduction: Versican is a large extracellular matrix (ECM) proteoglycan that regulates adhesion, survival, proliferation and migration of the cells, as well as ECM assembly. In rodent follicles, versican V0/V1 expression increases about 4 h after hCG induction, while in bovine granulosa cells no increase was observed post hCG injection, suggesting that versican expression may varies significantly between species. Interestingly, the versican V1 cleaved product G1-DPEAA accumu...

ra0003p033 | (1) | SRF2016

Determining the effect of extra-villous trophoblast cells on spiral artery remodelling: What is the role of MMP10?

Sayer Ben , Ashton Sandra , Cartwright Judith E , Whitley Guy

Background: Spiral artery remodelling is crucial for a successful pregnancy. In a healthy human pregnancy, cells of the placenta called extravillous trophoblasts (EVT) invade into the decidua and interact with the endothelial cells (EC) and vascular smooth muscle cells (VSMC) of the maternal spiral arteries (SA) in a process known as spiral artery remodelling. This process results in the development of low-resistance, dilated SAs that increase the blood supply to the developin...

ra0003p034 | (1) | SRF2016

Geographical variation in canine testicular environmental chemicals: a possible link with altered reproductive development

Sumner Rebecca , O'Sullivan Phoebe , Coley Natasha , Byers Andrew , Moxon Rachel , England Gary , Zhang Zulin , Lea Richard

Introduction: Environmental chemicals (ECs) are associated with an increased incidence of testicular cancer and reduced sperm quality. Regional differences are thought to reflect different levels of EC exposure. Since the dog is a sentinel of human exposure, we hypothesised that geographical variation in testicular chemical profiles may be associated with altered reproductive development and/or function.Methods: Canine adult testes (routine castrations) ...

ra0003p035 | (1) | SRF2016

Female reproductive ageing: When during oogenesis do chromosomes begin to fall apart?

Mejia Randy Ballesteros , Lister Lisa , Cooney Daniel , Herbert Mary

Introduction: During female fetal life, mammalian oocytes begin meiosis and arrest in prophase of meiosis I when they become enclosed in primordial follicles. Bivalent chromosomes established during this time have to remain intact until they are resolved during anaphase I, just before ovulation. Bivalents are stabilized throughout this period by Rec8-containing cohesin complexes. In women, however, extending this period for more than 35 years increases the risk of aneuploidy r...

ra0003p036 | (1) | SRF2016

Cellular modeling of citrin deficiency using human induced pluripotent stem cell-derived hepatocytes

Han Yong-Mahn , Kim Yeji , Lee Beom-Hee , Yoo Han-Wook

Citrin deficiency (CD) is a recessive genetic disorder caused by mutations in SLC25A13 gene which encodes citrin protein. CD patients manifest various symptoms related to nutrient metabolism such as urea cycle failure, abnormal amino acid levels, and fatty liver. To understand the pathophysiology of CD, the molecular phenotypes were investigated using induced pluripotent stem cells (iPSCs) derived from dermal fibroblasts of CD patient (CD-iPSCs). Here we demonstrate t...

ra0003p037 | (1) | SRF2016

Disease takes its Toll on reproduction: Toll-like receptors and the bovine corpus luteum

Nicholls Annemieke , Payne Loren , Robinson Robert , Coffey Tracey , Woad Katie

Introduction: Toll-like receptors (TLRs) are critical mediators of the host defence against pathogens, but may also directly influence reproduction. TLR signalling pathways have been identified in bovine granulosa cells. However, despite key diseases, such as mastitis, being associated with reduced luteal function, TLRs have not been extensively examined in the corpus luteum (CL). TLRs have also been implicated in tissue repair and remodelling processes critical to the rapid t...

ra0003p038 | (1) | SRF2016

The impact of the selective progesterone receptor modulator (SPRM), ulipristal acetate (UPA) administration upon cell proliferation markers within the human endometrium

Matthews Rebecca , Murray Alison , Whitaker Lucy , Millar Michael , Nicol Moira , Williams Alistair , Critchley Hilary

Introduction: Selective progesterone receptor modulators (SPRMs) have been reported to decrease cell proliferation within uterine fibroids, and reduce menstrual blood loss. The SPRM Ulipristal Acetate (UPA) has an anti-proliferative effect on fibroid tissue, yet its impact on endometrial cell proliferation is not well understood. The aim of this study was to quantify the effects of the SPRM UPA administration on endometrial cell proliferation within the human endometrium.<...

ra0003p039 | (1) | SRF2016

Mouse follicles have a smaller follicular antrum in the absence of oocyte core 1-derived O-glycans at two weeks of age

Shard Amelia , Williams Suzannah , Ploutarchou Panayiota

Introduction: A role for oocyte core 1-derived O-glycans in the regulation of ovarian follicle development has previously been shown using the C1galt1F/F:ZP3Cretransgenic mouse model. In this model, oocyte-specific ablation of core 1-derived O-glycans results in a sustained increase in ovulation rate and fertility, which is hypothesized to be due to changes in follicle development. In this study, we sought to characterise the role of oocyte core 1-derived O-glycans ...

ra0003p040 | (1) | SRF2016

SIRT1 regulates low oxygen induced bovine granulosa cell proliferation through interaction with VEGF-AKT-mTOR pathway

Shiratsuki Shogo , Hara Tomotaka , Shirasuna Koumei , Kuwayama Takehito , Iwata Hisataka

Introduction: Proliferation of granulosa cells (GCs) is essential for oocyte growth toward ovulation. Extreme proliferation of GCs is taken place under hypoxic condition due to lack of vascularization in the follicles. However, the mechanisms underlying proliferation of GCs under hypoxia have not been elucidated. In the present study, we examined the effect of low oxygen level on molecular background of cellular proliferation in bovine GCs.Methods: Bovin...

ra0003p041 | (1) | SRF2016

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ra0003p042 | (1) | SRF2016

Comparative investigation of lipid peroxidation and antioxidant enzymes in relation to semen quality

Riaz Muhammad , Mahmood Zahed , Saeed Muhammad Usman Qamar , Shahid Muhammad

Introduction: Lipid peroxidation is considered as an important mechanism of male infertility causing impairment in functioning of spermatozoa. The main aim of the study was to investigate the lipid peroxidation and antioxidant enzymes in relation to semen quality.Materials and methods: For this study total oxidant status (TOS), total antioxidant status (TAS), paraoxonase (PON), arylesterase and malondialdehyde (MDA) were investigated in seminal plasma an...

ra0003p043 | (1) | SRF2016

Pup sex and body mass of rats raised in different lactation litter sizes affect ghrelin and peptide-YY concentrations

Johnson Michelle L , Saffrey M Jill , Taylor Victoria J

Introduction: Previous studies have established that litter size during lactation influences body size and adiposity in male rat pups, but female pups and appetite-regulatory hormones have not been studied in this way. Gastrointestinal hormones ghrelin and peptide-YY (PYY) have roles in appetite regulation: high ghrelin levels signal hunger high PYY levels signal satiety. Both hormones are also associated with altered body mass and body composition. Previous findings (SRF 2014...

ra0003p044 | (1) | SRF2016

Effect of aflatoxin B1, on sperm vitality, mitochondrial function and acrosome reaction: the bovine model

Saktsier Moty , Komsky-Elbaz Alisa , Roth Zvi

Introduction: Aflatoxins are poisonous by-products from soil-borne fungus Aspergillus flavus, which involved in the decomposition of plant materials. Various food products, such as maize, sorghum, millet, rice and wheat, are contaminated with aflatoxins. Aflatoxin B1 (AFB1) is the most toxic aflatoxin and classified as carcinogen and mutagen. The lethality and toxicity extent of AFB1 varies between animals and organs. In the current study we examined the effects on sperm funct...

ra0003p045 | (1) | SRF2016

A Bayesian view of rodent seminal cytokine networks

Johnson Michelle L. , Dasgupta Tathagata , Gopichandran Nadia , Field Sarah L. , Orsi Nicolas M.

Introduction: It is understood that active agents in seminal fluid are key to initiating and coordinating mating-induced immunomodulation. This study aimed to characterise the structure of a network of cytokines whose interactions are thought to underpin this process in rats and mice.Methods: Seminal fluid, collected from isolated seminal glands, and serum, collected by cardiac puncture, were obtained from sexually mature Wistar rats (n=20) and ...

ra0003p046 | (1) | SRF2016

Dynamic changes in expression and DNA methylation of the astrocyte-specific genes Slc1a2 and Lrg1 during development: implications for preterm birth?

Allen Caroline , Cartier Jessy , Drake Amanda

Introduction: Preterm birth associates with later neurological conditions. Many of the underlying mechanisms are unknown however studies suggest changes in DNA methylation may be important. We have previously identified differential DNA methylation at astrocyte-specific genes, Slc1a2 and Lrg1 in preterm infants. Here we set out to describe the normal developmental patterns of gene expression and DNA methylation of Slc1a2 and Lrg1 in vivo in an animal model and assessed the pot...

ra0003p047 | (1) | SRF2016

Germ cells, from a mouse model of Premature Ovarian Failure, retain the potential to support follicle development when reaggregated with wildtype somatic cells

Sheikh Sairah , Kaune Heidy , Deleva Anna , Williams Suzannah

Introduction: Premature ovarian failure (POF) is a condition that affects ~1% of women and is idiopathic in 74–90% of cases. Our mouse model of POF, the Double Mutant (DM), results from oocyte-specific ablation of core 1-derivedO-glycans and complex and hybrid N-glycans. DM females are subfertile at 6-weeks of age and infertile at 9-weeks of age. By 3 months, DM females exhibit POF with ovaries containing fewer developing follicles but more primary 3a follicles. We invest...

ra0003p048 | (1) | SRF2016

Effect of temperature on bovine granulosa cells cultured under low oxygen in the presence or absence of melatonin

Zeebaree Bayar , Kwong Wing Yee , Mann George , Gutierrez Carlos , Sinclair Kevin

Heat stress can impair ovarian function and reduce fertility in cattle. It is correlated with oxidative stress and can induce granulosa cell apoptosis and reduce steroid production. Effects of heat stress can be reversed by melatonin, but studies with ovarian cells have only been evaluated under atmospheric oxygen tension. Here we cultured granulosa cells (GCs) from antral follicles in fibronectin-coated 12-well plates in M199 for up to 144h under physiological (5%) oxygen ten...

ra0003p049 | (1) | SRF2016

PGF2alpha regulates the expression of genes involved in embryo-maternal interactions in the porcine endometrium and conceptus cells

Kaczynski Piotr , Baryla Monika , Waclawik Agnieszka

Introduction: Proper interactions between uterus and conceptuses are necessary for establishment of pregnancy and implantation. Key factors in these regulations are prostaglandins (PGs). Recently we presented an important role of prostaglandin F2α (PGF2α) and its receptor (PTGFR) in porcine endometrium during early pregnancy. The aim of present study was to determine whether PGF2α can regulate the endometrial and embryonic expression of biglycan (BGN), metallopr...

ra0003p050 | (1) | SRF2016

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ra0003p051 | (1) | SRF2016

The effect of macrophage colony-stimulating factor (CSF1) on piglet gonad development

Ashworth Cheryl , Avey Billie , Stenhouse Claire , Sauter Kristin , Waddell Lindsey , Hogg Charis , Lisowski Zofia , Hume David

Introduction: CSF1 is the primary growth factor required for macrophage proliferation and differentiation. In adults, the receptor is expressed only in macrophages and their precursors. In mice, mutation of the CSF1 gene produces both male and female infertility due to diminished gonadal steroid production. We tested the effect of increased availability of CSF1 on gonadal development in pigs using CSF1-Fc, a CSF1 fusion protein with increased circulating half-life.<p class...

ra0003p052 | (1) | SRF2016

Histological categorization of equine ovarian follicles from healthy and diseased mares

Al Ibrahim Abdulqader , Tay Su Wei , Parkin Patrick Pollock Timothy , Carmichael Monika Mihm

Antral follicle waves in mares are considered a model for ovarian function in women, and can be sampled with relative ease in vivo or following ovary removal to investigate the regulation of follicle growth. To begin determine the effects of disease which may dysregulate follicular granulosa (GC) or theca cell (TC) proliferation, this study characterised 41 antral follicle wall H&E sections recovered from ovaries of 16 mares that were classified as healthy or suffered from...

ra0003p053 | (1) | SRF2016

The effect of dietary protein level on bovine follicular dynamics in beef heifers

Edwards Jennifer , Mongan Nigel , Rutland Catrin , Perry Viv , Robinson Robert

Introduction: Periconception dietary protein can affect fertility in cattle. This study investigated the effect of dietary crude protein intervention on gene expression in granulosa cells, metabolite concentrations in follicular fluid and serum anti-Müllerian hormone (AMH) concentrations.Methods: Non-pregnant Angus cross heifers (n=320) were group fed an isocalorific low (LP 10%) or high (HP 14%) crude protein diet for >60 days prior to...

ra0003p054 | (1) | SRF2016

Inhibition of macrophage colony-stimulating factor-1 (CSF-1) receptor signalling: a novel therapeutic target for tubal ectopic pregnancy

Beaty Robyn , Ahmad S Furquan , Campbell Lisa , Horne Andrew

Introduction: An ectopic pregnancy (EP) is defined as a conceptus implanting outside of the uterine cavity, most commonly in the Fallopian tube. It is a life-threatening gynaecological condition with limited treatment options. Large and unstable EP are managed by surgical excision. Smaller stable EP are medically managed with the chemotherapeutic drug methotrexate. Methotrexate has considerable side effects and a high treatment failure rate (~30%). There is an unmet medical ne...

ra0003p055 | (1) | SRF2016

Putative role for progesterone in Monodelphis domestica embryogenesis and pregnancy

Cruz Yolanda , Yoshida Karin , Griffith Kobi , Johnson Joanna

Introduction: Progesterone (P) has an unusual role in didelphid marsupials in that pheromonally induced levels of pro-estrous P trigger ovulation. Work in our lab reveals that serum P levels are significantly higher on pregnancy day 3 in the didelphid, Monodelphis domestica, plummet to extremely low levels during pregnancy day 5, and return to day-3 levels on day 9, before gradually declining as pregnancy advances. Because this precipitous decline coincides with the h...

ra0003p056 | (1) | SRF2016

Male infertility-linked point mutation dramatically reduces the Ca2+ oscillation-inducing activity of sperm PLCzeta without affecting its ability to hydrolyse PIP2

Nomikos Michail , Stamatiadis Panagiotis , Sanders Jessica , Calver Brian Lewis , Lofty Morgan , Buntwal Luke , Swann Karl , Lai Francis Anthony

Introduction: Sperm-specific phospholipase C zeta (PLCζ) is widely considered to be the physiological stimulus that evokes intracellular calcium (Ca2+) oscillations that are essential for the initiation of egg activation and early embryonic development during mammalian fertilization. Sperm-delivered PLCζ hydrolyses phosphatidylinositol 4,5-bisphosphate (PIP2) stores within the ooplasm, triggering Ca2+ oscillations through the inositol...

ra0003p057 | (1) | SRF2016

Antigen unmasking improves visualisation efficacy of phospholipase C zeta (PLCζ) in mammalian sperm to enable diagnostic applicability for evaluating PLCζ-dependent human oocyte activation deficiency

Kashir Junaid , Buntwal Luke , Nomikos Michail , Calver Brian , Stamatiadis Panagiotis , Ashley Peter , Sanders David , Knaggs Paul , Bunkheila Adnan , Swann Karl , Lai Francis Anthony

Introduction: Mammalian oocyte activation is mediated via a series of intracellular calcium oscillations induced by a sperm-specific phospholipase C, PLCzeta (PLCζ). PLCζ presents significant promise as a clinical therapeutic for some forms of male infertility. However, the utility of PLCζ as a potent diagnostic tool for human sperm remains undefined. Furthermore, considerable variation in reported PLCζ localization patterns in sperm highlight the necessity...

ra0003p058 | (1) | SRF2016

The effect of transforming growth factor B on luteal angiogenesis and function in vitro

Thompson Robert , Woad Kathryn , Robinson Robert

Introduction: The formation of a functional corpus luteum (CL) is integral to the establishment and maintenance of pregnancy. The development of the CL requires tightly regulated angiogenesis, which is controlled by a plethora of pro and anti-angiogenic factors. Transforming growth factor B (TGFB) has been identified as a potential mediator of this process. This study tested the hypothesis that TGFB would adversely impact on endothelial cell (EC) development and reduce the ste...

ra0003p059 | (1) | SRF2016

Insight into the molecular mechanisms underlying enhanced gonadotropin hormone receptor activity in polycystic ovarian syndrome

Owens Lisa , Lerner Avi , Christopoulos George , Liyanage Maneshka , Islam Rumana , Lavery Stuart , Tsui Vicky , Hardy Kate , Franks Stephen , Hanyaloglu Aylin

Introduction: Polycystic ovary syndrome (PCOS) is a common endocrine disorder, affecting 5–10% of women of reproductive age, and is the major cause of anovulatory infertility and hyperandrogenism. Aberrant secretion and or action of gonadotropins are implicated but, to date, we have only limited knowledge about the precise mechanisms that are involved. Recent genome wide association studies have discovered significant signals have emerged at loci close to the genes on chr...

ra0003p060 | (1) | SRF2016

Effect of in vitro fertilization (IVF) and embryo culture duration on mouse development and postnatal health

Aljahdali Anan , Khalif Ili Raja , Velazquez Miguel A , Sheth Bhav , Smyth Neil R , Fleming Tom

Since the advent of IVF (in vitro fertilisation), several million babies have been born worldwide. However, reports link in vitro techniques with adverse short and long-term health outcomes. Using a mouse model, we investigated the effect of IVF and duration of culture on blastocyst development and cell number and the postnatal health of offspring. Experimental groups (8–13 litters each): NM (natural mating, non-superovulated) IV-ET-2Cell (2-cell embryos...

ra0003p061 | (1) | SRF2016

Altered expression of genes affecting oestrogen metabolism and action in granulosa-lutein cells of women with PCOS

Coates Melanie , Lerner Avigdor , Christopoulos Georgios , Liyanage Maneshka , Lavery Stuart , Tsui Victoria , Hardy Kate , Franks Stephen

Introduction: Polycystic ovary syndrome (PCOS) is a common endocrinopathy in premenopausal women and is associated with hyperandrogenism, anovulatory infertility, and metabolic abnormalities. Abnormalities in the steroidogenic pathway of both theca and granulosa cells have previously been reported, but there has been no comprehensive analysis of steroidogenic gene expression in granulosa-lutein (GL) cells of women with PCOS. In this study, we investigated a panel of genes invo...

ra0003p062 | (1) | SRF2016

Abstract withdrawn....

ra0003p063 | (1) | SRF2016

Preantral follicle development in cultured reaggregated neonatal ovaries

Lo Belinda , Sheikh Sairah , Williams Suzannah

Introduction: Follicle development is complex, and the use of reaggregated ovaries (ROs) allows us to investigate oocyte-somatic cell interactions, since they can be created using different sources of germ and somatic cells. Production of an RO involves the separation of germ and somatic cells using differential plate adhesion, followed by reaggregation into a pellet. The pellet is then transplanted beneath the kidney capsule of an immunocompromised mouse which facilitates fol...

ra0003p064 | (1) | SRF2016

First evidence of a menstruating rodent: the spiny mouse (Acomys cahirinus)

Bellofiore Nadia , Ellery Stacey , Mamrot Jared , Walker David , Temple-Smith Peter , Dickinson Hayley

Background: Menstruation, the cyclical breakdown of the superficial endometrial layer in the absence of pregnancy, occurs in 1.5% of mammals. There is no obvious phylogenetic link between species known to menstruate: humans, Old World monkeys, some bats and the elephant shrew, and true menstruation has never before been reported in rodents. Observations of blood at the vaginal opening in some females, led us to examine the possibility that the spiny mouse (Acomys cahirinus...