Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0003p017 | (1) | SRF2016

Treatment of GDF8 during in vitro maturation increased phosphorylated SMAD2/3 and improved in vitro fertilized embryo developmental competence

Yoon Junchul David , Hyun Sang-Hwan

The purpose of this study is the effects of growth differentiation factor 8 (GDF8) and SB431542 (SB) on porcine oocytes in vitro maturation (IVM), subsequent embryonic development after in vitrofertilization (IVF). We were investigated the effect of GDF8 and SB treatment during IVM on nuclear maturation, intracellular glutathione (GSH), reactive oxygen species (ROS) levels, analyzed specific gene transcription and translation levels in cumulus cells after IVM, and emb...

ra0003p018 | (1) | SRF2016

Establish of a transgenic neural cell line with an astrocyte-specific inducible CreERT2 system

Hwang Seon-Ung , Yoon Junchul David , Han Yongquan , Eun Kiyoung , Kim Hyunggee , Hyun Sang-Hwan

Transgenic (TG) pigs are currently regarded as an important animal model for various biomedical researches including a disease modeling and a regenerative medicine. We tried to develop a pig astrocyte-specific CreERT2-LoxP recombination system as a part of TG pig model of brain tumor arisen from astrocytic cell lineage. We designed two vector systems one retains pig glial fibrillary acidic protein (GFAP) promoter-CreERT2 transgene and the other has GFP ge...