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Society for Reproduction and Fertility Annual Conference 2015

Oxford, UK
20 Jul 2015 - 22 Jul 2015

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Oral Communications 1: Embryo and Implantation

ra0002o004 | Oral Communications 1: Embryo and Implantation | SRF2015

The bioenergetic profile of the bovine blastocyst

McKeegan Paul J , Sturmey Roger

The preimplantation embryo undergoes dynamic changes in energy demand during development. Oxygen consumption rate (OCR), representing overall oxidative metabolism, has been reported in several species but few studies have examined the components of OCR. Individual blastocyst OCR was measured using a non-invasive oxygen probe (unisense), whereas OCR of grouped blastocysts was determined using the Oxygen Biosensor fluorometric assay (BD Bioscience). Respiratory chain inhibitors ...

ra0002o005 | Oral Communications 1: Embryo and Implantation | SRF2015

Effect of environmental oxygen on the expression of miRNAs in human embryonic stem cells

Sander Sophia P , Sanchez-Elsner Tilman , Houghton Franchesca D

Human embryonic stem cells (hESCs) are derived from the inner cell mass of the blastocyst. They proliferate by self-renewal and have the potential to develop into all cells of the three germ layers. Thus, hESCs hold great potential for use in regenerative medicine. hESCs are difficult to maintain in culture and have a tendency to spontaneously differentiate. Culture at a low, 5% oxygen concentration is beneficial for the maintenance of a wholly pluripotent cell population comp...

ra0002o006 | Oral Communications 1: Embryo and Implantation | SRF2015

Mechanism of microRNA lethal-7 in embryo dormancy in mice

Liu Weimin , Pang Ronald T K , Chiu Philip C N , Li Qian , Yeung William S B

Embryo dormancy (embryo diapause) is a reproductive strategy used by ~100 different mammals to avoid the risk of exposing their offsprings to unfavourable environmental conditions by delaying embryo implantation. The blastocysts from domestic sheep, rabbit or cattle enter into diapause after being transfer into mouse uteri induced to have delayed implantation. The observation suggests that blastocysts from all mammals, including that of human, may have an ability to enter into...

ra0002o007 | Oral Communications 1: Embryo and Implantation | SRF2015

A potential co-culture system of ovine blastocyst with uterine endometrium to mimic initial attachment of embryo implantation

Tremaine Tina , Fouladi-Nashta Ali A , Wathes D Claire , Khalid Muhammad

In livestock ruminant species including sheep and cattle, high fertilisation rates shown around 80%, do not necessarily equate to successful pregnancy. Deficient uterine function is therefore a major contributory factor to pregnancy failure resulting in embryonic mortality. In humans undergoing IVF treatment for subfertility, approximately only 25% of embryo transfers will successfully implant. To gain a better understanding of the natural implantation environment, in vitr...

ra0002o008 | Oral Communications 1: Embryo and Implantation | SRF2015

The effect of IVF and embryo culture on mouse development and adult health

Aljahdali Anan , Raja Khalif Raja Ili Airina , Velazquez Miguel Abraham , Sheth Bhav , Smyth Neil , Fleming Tom

Several million babies have been born worldwide since IVF and assisted-reproductive technologies (ART) became available. However, reports link IVF techniques with adverse short- and long-term health outcomes.1,2,3 Using a mouse model, we investigated the effect of IVF on the total cell number in blastocyst stage embryos and the postnatal health of offspring.Methods: Experimental groups; (NM) C57/BL6 non-superovulated females naturally mated wi...