Searchable abstracts of presentations at key conferences on reproductive biology and medicine
Previous issue | Volume 3 | SRF2016

Society for Reproduction and Fertility Annual Conference 2016

Winchester, UK
11 Jul 2016 - 11 Jul 2016

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Oral Communications 1: Ovary

ra0003o001 | Oral Communications 1: Ovary | SRF2016

Mice with follicular premature ovarian failure at 3 months of age become a follicular by 1 year of age

Plumb Betsy , Sheikh Sairah , Grasa Patricia , Ploutarchou Panayiota , Williams Suzannah

Introduction: Premature ovarian failure (POF) is a reproductive disorder which causes defects within the ovaries, normally leading to infertility. The disorder affects 1% of women under the age of 40 with 75% of cases with no known cause, indicating the need for more research. The Double Mutant (DM) mouse model with oocyte-specific deletion of C1galt1 and Mgat1 exhibit follicular POF at 3 months of age. Therefore, this study aimed to investigate the effect of ovarian dysfuncti...

ra0003o002 | Oral Communications 1: Ovary | SRF2016

The effect of folic acid supplementation during the juvenile pubertal period or adulthood on gene expression in the ovary

Escarate Reyna Penailillo , Burton Mark , Burdge Graham , Eckert Judith , Fleming Tom , Lillycrop Karen

Introduction: Women with mutations in BRCA1 gene have an increased lifetime risk of ovarian cancer. Many studies but not all have shown that an adequate folate intake is protective against many cancers including ovarian, but recent studies have shown that high levels of folic acid (FA) supplementation can promote cancer risk. The aim of this study is to determine FA impact on the expression of cancer related genes in the ovary such as OCT4 a pluripotency factor, BRCA1 related ...

ra0003o003 | Oral Communications 1: Ovary | SRF2016

PLCz-induced Ca2+ oscillations are enhanced after germinal vesicle breakdown during mouse oocyte maturation

Sanders Jessica , Bateson Ethan , Yu Yuansong , Nomikos Michail , Lai Antony , Swann Karl

Introduction: Mature mouse oocytes, arrested at meiotic metaphase II (MII), are activated by a series of Ca2+ oscillations caused by sperm-specific phospholipase C zeta (PLCz) which has been shown to localize in cytoplasmic vesicles. The substrate for PLCz, phosphatidylinositol 4,5-bisphosphate (PIP2), is also present in cytoplasmic vesicles in MII oocytes. Throughout oocyte maturation there is reported to be a gradual increase in sensitivity to PLCz and ...

ra0003o004 | Oral Communications 1: Ovary | SRF2016

Oocyte maturation arrest in endometriosis is caused by elevated levels of Reactive Oxygen Species and enforced via a DNA Damage Response and the Spindle Assembly Checkpoint pathway

Hamdan Mukhri , Jones Keith , Cheong Ying , Lane Simon

Introduction: It has recently been demonstrated that mouse oocytes respond to DNA damage by arresting in Meiosis I. This arrest has been shown to require the activity of the Spindle Assembly Checkpoint (SAC) and the DNA Damage Response (DDR). Given oocytes lack the equivalent of a G2/M checkpoint for DNA damage, the SAC response may be critical to defend against formation of DNA damaged embryos. It is currently unknown whether the DDR SAC pathway is sensitive to physiological ...

ra0003o005 | Oral Communications 1: Ovary | SRF2016

A Positive Feedback Loop between Hypoxia and miRNA-210 Augments Endothelin 2 in Human Granulosa Cells

Shrestha Ketan , Esinberg-Loebl Iris , Onasanya Adepeju Esther , Greenfield Caryn , Yalu Ronit , Imbar Tal , Meidan Rina

Introduction: Hypoxia and Endothelin-2 (EDN2) peak simultaneously during early corpus luteum (CL) formation, suggesting causal relationship. Indeed, hypoxia inducible factor-1 alpha (HIF1A) is a strong stimulator of EDN2 in granulosa–lutein cells (GLCs). Knockout of EDN2 resulted in un-ruptured follicles that failed to develop into CL, suggesting that EDN2 plays essential roles during ovulation. In agreement, EDN2 was low in GLCs of women with PCOS, characterized by ovula...

ra0003o006 | Oral Communications 1: Ovary | SRF2016

Post-partum endometritis: Its negative impact on luteal vascularisation, progesterone content and steroidogenic enzyme expression in the cow

Mohammed Zeravan , Mann George , Robinson Robert

Introduction: In dairy cows, post-partum endometritis caused by Gram negative bacteria (e.g. E. coli) adversely affects follicular function and is associated with subfertility. However, there is limited information of the effects of endometritis on the corpus luteum (CL). Recently, we showed that LPS dose-dependently decreased luteal endothelial cell (EC) network formation in vitro. The hypothesis tested was that CL from cows with endometritis would have reduced vascu...