Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p235 | (1) | WCRB2014

Tamoxifen inducible Cre systems: adverse effects of low dose tamoxifen treatments on the male reproductive tract

Patel Saloni H , Milne Laura , Sharpe Richard , Smith Lee B

Introduction: Tamoxifen inducible Cre systems have been used to study development specific roles of genes in the testis as they allow tight temporal control of genetic manipulation. However, tamoxifen is an anti-estrogen that competitively binds estrogen receptors. Despite the antagonistic properties of tamoxifen, it also acts as a weak estrogen agonist, hence exerting estrogenic effects in a tissue and cell specific manner. Given the duality of tamoxifen function and the impo...

ra0001p252 | (1) | WCRB2014

Roles of testicular macrophage in Leydig cell proliferation in colony-stimulating factor treated mice

Tsai Tina , Sauter Kristin , Milne Laura , Hume David , Smith Lee

Introduction: Leydig cells (LCs), the key producer of testosterone (T) in the testis, are affected by various factors such as hormone stimulation. There are various testicular cells that also affect LC function such as testicular interstitial macrophages (TIMs). TIMs are immune factor-producing cells, located adjacent to the LCs, sharing a close physiological and functional relationship. TIMs are associated with development and function of LCs. Ablation of TIMs have shown to i...

ra0001p212 | (1) | WCRB2014

Androgen receptor signalling in testicular Leydig cells is essential for Leydig cell maturation and survival

O'Hara Laura , McInnes Kerry , Simitsidellis Ioannis , Morgan Steph , Milne Laura , Mitchell Rod , Smith Lee

Androgen receptor (AR) is expressed by testicular somatic cells including steroidogenic Leydig cells (LC). Its role in LC is ill defined, with hypotheses based on evidence from total androgen receptor knockout models implying that LC AR is responsible for final LC number, regulation of steroidogenic enzyme expression and LC maturation.To better define the role of AR in LC, we generated a mouse line with a LC specific ablation of AR (LCARKO) using the Cre...

ra0001p247 | (1) | WCRB2014

Effect of Sertoli cell ablation on testicular function in adult mouse

Rebourcet Diane , O'Shaughnessy Peter , Monteiro Ana , Mitchell Rod , Milne Laura , Smith Lee

The aging of western societies, and the associated increase in obesity, brings with it an increase in prevalence of disorders such as metabolic syndrome which are associated with reduced androgen levels in men. As Leydig cells are the source of androgens in the male, establishing the mechanisms which control Leydig cell development and function is crucial to our understanding of aging and male health. Sertoli cells regulate testicular fate in the differentiating gonad and are ...

ra0001p332 | (1) | WCRB2014

Androgen receptor-signalling in the male pituitary is dispensable for normal LH secretion but suppresses prolactin production

Ferreira Maria Tedim , Curley Michael , Milne Laura , Smith Lee , O'Hara Laura

Production of testosterone by testicular Leydig cells is under tight regulation by the hypothalamic – pituitary – gonadal (HPG) axis. GnRH, secreted by the hypothalamus, stimulates secretion of LH from the pituitary. LH will then stimulate testosterone production in the Leydig cells, which will feedback to the hypothalamic-pituitary element to inhibit LH secretion.Previously it was accepted that testosterone exerted its negative feedback action...