(1)

Mitochondrial dynamics controlled by mitofusins direct organelle positioning and movement during meiotic division

Wakai Takuya Harada Y Miyado K Kono Tomohiro

¹Tokyo University of Agriculture, Tokyo, Japan; ²National Center for Child Health and Development, Tokyo, Japan.

ra0001p031

The detailed localization of meiotic cohesin subunits, RAD21L and REC8, in mouse spermatocytes

Rong Mei Matsuda Atsushi Hiraoka Yasushi Lee Jibak

¹Kobe University, Kobe, Japan; ²National Institute of Information and Communications Technology Advanced ICT Research Institute, Kobe, Japan; ³Graduate School of Frontier, Biosciences Osaka University, Suita, Japan.

ra0001p032

Atrazine exposure of adult mice affect spermatogenesis

Gely-Pernot Aurore Hao Chunxiang Kervarec Christine Stuparevic Igor Chalmel Frederic Evrard Bertrand Priming Michael Jegou Bernard Smagulova Fatima

Inserm UMR1085, IRSET, Université Rennes1, Rennes, France.

ra0001p033

C-type natriuretic peptide stimulates resumption of meiosis via a cGMP-dependant mechanism in porcine oocytes

Rose Ryan D Sugimura Satoshi Ritter Lesley J Brown Hannah M Thompson Jeremy G Gilchrist Robert B

¹Robinson Research Institute, The University of Adelaide, Adelaide, South Australia, Australia; ²Tokyo University of Agriculture and Technology, Fuchu, Japan; ³University of New South Wales, Randwick, New South Wales, Australia.

ra0001p034

Zinc regulate meiotic resumption and metaphase arrest in porcine oocyte

Zhao Ming-Hui Kwon Jung-Woo Liang Shuang Kim Seon-Hyang Kim Teoan Kim Nam-Hyung Cui Xiang-Shun

¹Chungbuk National University, Cheongju, Republic of Korea; ²Catholic University of Daegu, Daegu, Republic of Korea.

ra0001p035

Morphological markers to select populations of oocytes with different cultural needs for dedicated pre-maturation systems

Dieci Cecilia Labrecque Remi Lodde Valentina Tessaro Irene Baruffini Valentina Lodi Giorgia Modina Silvia Sirard Marc-Andre Luciano Alberto M

¹University of Milan, Milan, Italy; ²Université Laval, Ville de Québec, Québec, Canada.

ra0001p036

Abstract unavailable

ra0001p037

The gametic synapse; transferring RNA to the oocyte

Macaulay Angus D Gilbert Isabelle Caballero Julieta Scantland Sara Fournier Eric Tossou Prudencio Sirard Marc-Andre Clarke Hugh Khandjian Edward Richard Francois Hyttel Poul Robert Claude

Laval, Ville de Québec, Québec, Canada; ²McGill University, Montreal, Québec, Canada; ³Institut Universitaire en Santé Mentale de Québec, Quebec City, Québec, Canada; ⁴University of Copenhagen, Copenhagen, Denmark.

ra0001p038

Dissecting the meiotic gene network in female embryonic germ cells

Abby Emilie Tourpin Sophie Guerquin Marie-Justine Le Bouffant Ronan Habert Rene Livera Gabriel

CEA/INSERM/Universities P7-P11, Fontenay aux Roses, France.

ra0001p039

The oocyte influences cumulus expansion and glucose metabolism during IVM in cattle

Lima Paula F Price Christopher A Buratini Jose

¹UNESP, Botucatu, Brazil; ²Université de Montreal, Saint-Hyacinthe, Quebec, Canada.

ra0001p040

Plk1 plays a role in cortical actin polymerisation during meiosis I

Yuen Wai Shan Zhang Qing-Hua Carroll John

Monash University, Clayton, Victoria, Australia.

ra0001p041

Cyclin A2 is essential for the chromosome segregation during the meiosis I of the mouse oocyte maturation

Zhang Qing-Hua Yuen Wai Shan Carroll John

Monash University, Clayton, Victoria, Australia.

ra0001p042

Effect of cAMP regulators on bovine cumulus-oocyte communication and embryo development in vitro

Nogueira Marcelo G Machado Mariana F Li Haijun Razza Eduardo M Gilchrist Robert B Sutton-McDowall Melanie L Thompson Jeremy G

¹UNESP, Assis, Brazil; ²UNESP, Botucatu, Brazil; ³College of Veterinary Medicine, Hohhot, China; ⁴University of New South Wales, Randwick, New South Wales, Australia; ⁵The University of Adelaide, Adelaide, South Australia, Australia; ⁶The Robinson Institute, Adelaide, South Australia, Australia.

ra0001p043

Insight into progesterone receptor membrane component 1 action during bovine oocyte meiosis by means of siRNA-mediated gene silencing

Lodde Valentina Tessaro Irene Raucci Franca Franciosi Federica Marchese Federica Modina Silvia Peluso John J. Luciano Alberto M

¹University of Milan, Milan, Italy; ²University of California, San Francisco, San Francisco, California, USA; ³University of Connecticut Health Center, Farmington, Connecticut, USA.

ra0001p044

Maturation conditions do not affect Myst1, Hat1, and Sirt1 mRNA abundance in horse oocytes

Tessaro Irene Franciosi Federica Papillier Pascal Goudet Ghylene Dalbies-Tran Rozenn Douet Cecile Reigner Fabrice Deleuze Stefan Lodde Valentina Luciano Alberto M.

¹University of Milan, Milan, Italy; ²University of California, San Francisco, San Francisco, California, USA; ³INRA, UMR85 Physiologie de la Reproduction et des Comportements, Nouzilly, France; ⁴Université de Liège, Liege, Belgium.

ra0001p045

Inhibition of mTOR signaling induces cumulus expansion and stimulates meiotic maturation of oocytes in mice

Hoshino Yumi Sato Eimei

Tohoku University, Sendai, Japan.

ra0001p046

Inhibition of DMRTA2 impairs human female germline development in xeno-grafted ovaries

Poulain Marine Tourpin Sophie Muczynski Vincent Messiaen Sebastien Moison Delphine Duquenne Clotilde Benachi Alexandra Rouiller-Fabre Virginie Habert Rene Frydman Nelly Livera Gabriel

¹UMR967/ Univ P7&P11, Fontenay aux Roses, France; ²CEA/ INSERM/ Universities P7-P11, Fontenay aux Roses, France; ³Antoine Béclère Hospital, Clamart, France.

ra0001p047

Abnormal behavior of lysine acetylation during one-cell stage mouse cloned embryos

Kishigami Satoshi Reum Lee Ah Ha Le Thanh Morimoto Koichi Hosoi Yoshihiko

Kinki University, Kinokawa-shi, Japan.

ra0001p048

Nucleoli are formed in developing mouse embryos without nucleolus precursor bodies

Kyogoku Hirohisa Fulka Josef Kitajima Tomoya S Wakayama Teruhiko Miyano Takashi

¹RIKEN Center for Developmental Biology (CDB), Kobe, Japan; ²Institute of Animal Science, Prague, Czech Republic; ³University of Yamanashi, Kofu, Japan; ⁴Kobe University, Kobe, Japan.

ra0001p049

Fingerprints on oocyte and embryo lipid profiles caused by in vitro embryo production system and fatty acid diet supplementation in bovine

Gonzalez Serrano Andres Felipe Ferreira Christina R Pirro Valentina Lucas-Hahn Andrea Heinzmann Julia Hadeler Klaus-Gerd Aldag Patrick Baulain Ulrich Piechotta Marion Danicke Sven Cooks Robert Graham Niemann Heiner

¹Institute of Farm Animal Genetics (FLI), Neustadt, Germany; ²Purdue University, West Lafayette, Indiana, USA; ³University of Turin, Turin, Italy; ⁴University of Veterinary Hanover, Hannover, Germany; ⁵Institute of Animal Nutrition (FLI), Braunschweig, Germany.

ra0001p050

Degradation of estrogen receptor [alpha] in activated blastocysts is associated with implantation in the mouse

Matsumoto Hiromichi Saito Kyosuke Furukawa Etsuko Kobayashi Mitsuru Fukui Emiko Yoshizawa Midori

Utsunomiya University, Utsunomiya, Japan.

ra0001p051

Production of prion gene knockout cow to prevent spontaneous bovine spongiform encephalopathy

Manabe Noboru Onoyama Ichiro Li Junyou Sendai Yutaka Aoyagi Yoshito

¹The University of Tokyo, Kasama, Japan; ²National Federation of Agricultural Co-operative Associations, Tsukuba, Japan.

ra0001p052

The effect of lysophosphatidic acid during in vitro maturation of bovine cumulus-oocyte complexes: cumulus expansion, glucose uptake and expression of expansion-related genes

Boruszewska Dorota Sinderewicz Emilia Kowalczyk-Zieba Ilona Woclawek-Potocka Izabela

Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland.

ra0001p053

Essential roles of condensins in chromosome organization during mouse early embryogenesis

Lee Jibak Shimizu Moeko Nishide Kenji Hirano Tatsuya

¹University, Kobe, Japan; ²RIKEN, Wako, Japan.

ra0001p054

Effect of downregulating CDX2 transcript by RNA interference on early development of bovine embryos

Sakurai Nobuyuki Takahashi Kazuki Hashizume Tsutomu Sawai Ken

Iwate University, Morioka, Japan.

ra0001p055

Fine cryopreservation method of porcine blastocysts produced by in vitro fertilization

Min Sung-Hun Kim Jin-Woo Lee Yong-Hee Ahn Jae-Hyun Do Geon-Yeop Chae Sung-Kyu Park Humdai Koo Deog-Bon

Daegu University, Gyeongsan-si, Republic of Korea.

ra0001p056

In vivo embryo production in queens treated with deslorelin acetate (Suprelorin)

Louise Ackermann Camila Ferrari Crocomo Leticia Trevisol Eduardo Silva Rascado Tatiana Volpato Rodrigo Lopes Carlize de Freitas Guaitolini Carlos Renato de Almeida Costa Talita Lopes Maria Denise

São Paulo State University, Botucatu, Brazil.

ra0001p057

O-GlcNAcylation in pig embryos during preimplantation development

Shibutani Mihiro Miyano Takashi Miyake Masashi

Kobe University, Kobe, Japan.

ra0001p058

Importance of amino acids in the development of preimplantation mouse embryo

Zamfirescu Radu Shreedharan Salini Zada Mark Morris Michael Day Margot L

¹School of Medical Sciences, University of Sydney, Sydney, Camperdown, Australia; ²University of Sydney and Bosch Institute, Sydney, New South Wales, Australia.

ra0001p059

Thermoprotective action of IGF1 in embryos hamster 4-cells subjected to heat stress in vitro: effect of the redistribution of E-cadherin

Cordova Alfredo Trejo Benitez Ismael Abad Victor Manuel Meza Villalvazo del Carmen Navarro Maldonado Maria Garcia Demetrio Ambriz

¹Universidad del Papaloapan, Loma Bonita, Mexico; ²Universidad Autónoma Metropolitana-Iztapalapaa, Distrito Federal, Mexico.

ra0001p060

Effect of blastocyst artificial collapse prior to vitrification on pluripotency-specific genes expression in mouse embryos

Dashtizad Mojtaba Shamsara Mehdi Daliri Morteza Zandi Ghazaleh Fathalizadeh Parisa Hashemi Ehsan Hajarian Hadi

¹National Institute of Genetic Engineering and Biotechnology, Tehran, Iran; ²Razi University, Kermanshah, Iran

ra0001p061

The role of histone H3 variants in the alteration of chromatin structure after fertilization

Kawamura Machika Aoki Fugaku

The University of Tokyo, Kashiwa, Japan

ra0001p062

Roles of the untranslated regions in the translational regulation of cyclin A2 in murine oocytes and embryos

Mori Yuuka Aoki Fugaku

The University of Tokyo, Kashiwa, Japan

ra0001p063

The effects of PHE mixture, theophylline, and sperm concentrations on fertilization and development of bovine oocytes in vitro

Kang Sung-Sik Yanagawa Yojiro Nagano Masashi

Graduate school of Veterinary Medicine, Hokkaido University, Sapporo, Japan

ra0001p064

The effects of mesenchymal stem cell- conditioned medium on the fertilization rate, embryo development and OCT-4 gene expression of 8-cell embryo in NMRI mice

Azizi Alireza Shahbazi Majid Haidari Kamran

Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran

ra0001p065

The regulatory roles of let-7 in embryo dormancy in mice

Liu Weimin Pang Ronald T K Yeung William S B

The University of Hong Kong, Hong Kong

ra0001p066

Plasma growth hormone decline during early mammalian development is the result of expanding blood volume

Menzies Brandon R Hetz Jennifer Rao Alexandra Clarke Iain Shaw Geoff Renfree Marilyn B

¹The University of Melbourne, Melbourne, Australia; ²Monash University, Clayton, Australia

ra0001p067

Single-step generation of rabbits carrying a targeted allele using CRISPR/Cas9

Honda Arata Hirose Michiko Sankai Tadashi Yasmin Lubna Yuzawa Kazuaki Honsho Kimiko Izu Haruna Ikawa Masahito Ogura Atsuo

¹University of Miyazaki, Miyazaki, Japan, ²RIKEN BRC, Tsukuba, Japan, ³National Institute of Biomedical Innovation, Tsukuba, Japan, ⁴Research Institute for Microbial Diseases, Osaka University, Suita, Japan

ra0001p068

Supplementation of 6-diazo-5-oxo-l-norleucine improves in vitro maturation and in vitro developmental competence in pigs

Kim Ji-Su Yang Hae-Jun Jeong Pil-Soo Mun Seong-Eun Choi Seon-A Yoon Seung-Bin Park Young-Ho Song Bong-Seok Sim Bo-Woong Kim Sun-Uk Chang Kyu-Tae

KRIBB, Chungcheongbuk-do, Republic of Korea

ra0001p069

Treatment of fetal bovine serum improves early development of porcine embryos by alleviating oxidative stress

Choi Seon-A Mun Seong-Eun Jeong Pil-Soo Yang Hae-Jun Yoon Seung-Bin Park Young-Ho Song Bong-Seok Sim Bo-Woong Kim Ji-Su Kim Sun-Uk Chang Kyu-Tae Huh Jae-Won

KRIBB, Chungcheongbuk-do, Republic of Korea

ra0001p070

Hollow fiber vitrification of in vitro produced bovine embryos at early developmental stages

Uchikura Ayuko Matsunari Hitomi Nakano Kazuaki Matsumura Yukina Hatae Shota Asano Yoshinori Nagashima Hiroshi

¹Lab. of Developmental Engineering, Department of Life Sciences, Meiji University, Kawasaki, Japan; ²Meiji University International Institute for Bio-Resource Research (MUIIBR), Kawasaki, Japan

ra0001p071

Treatment of histone deacetylase inhibitor increases in vitro developmental competence of bovine cloned embryos through suppression of endoplasmic reticulum stress

Kim Sun-Uk Jeong Pil-Soo Yang Hae-Jun Mun Seong-Eun Choi Seon-A Yoon Seung-Bin Park Young-Ho Song Bong-Seok Sim Bo-Woong Kim Ji-Su Chang Kyu-Tae

KRIBB, Chungcheongbuk-do, Republic of Korea

ra0001p072

Early development of bovine embryos depends on the cooperative action between oxidative and endoplasmic reticulum stresses

Yoon Seung-Bin Yang Hae-Jun Mun Seong-Eun Jeong Pil-Soo Choi Seon-A Park Young-Ho Song Bong-Seok Sim Bo-Woong Kim Ji-Su Kim Sun-Uk Chang Kyu-Tae Lee Sang-Rae

KRIBB, Chungcheongbuk-do, Republic of Korea

ra0001p073

Novel porcine OCT4 variants identification in blastocyst and discriminative expression analysis in adult tissues

Hwang Jae Yeon Oh Jong-Nam Lee Dong-Kyung Lee Chang-Kyu

Seoul National University, Seoul, Republic of Korea.

ra0001p074

Chromatin remodelling gene Cecr2 in murine gametogenesis, fertilization, and early embryonic development

Norton Kacie A McDermid Heather E

University of Alberta, Edmonton, Canada

ra0001p075

Are cumulus cells additional players in calcium signalling during cattle oocyte fertilisation?

Sutton-McDowall Melanie L Thompson Jeremy G

¹Robinson Research Institute, Adelaide, Australia; ²ARC Centre of Excellence for Nanoscale BioPhotonics, Adelaide, Australia

ra0001p076

Pregnancy of bovine somatic cell nuclear transfer embryos reconstructed by using donor cells: iPS cell vs AID transfected cell*

Kim Tae Suk Baek Sang Ki Moon Song Yi Jin Sang Jin Ko Yeoung-Gyu Kim Sung Woo Park Hae-Geum Seong Hwan-Hoo Lee Joon Hee

¹College of Agriculture and Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea; ²Animal Development and Biotechnology Group, Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju, Republic of Korea; ³National Institute of Animal Science, Rural Development Administration, Namwon, Republic of Korea.

ra0001p077

The effect of cRNA concentration of artificial nuclease microinjected cytoplasmically to pronuclear porcine embryos on survival and development in vitro

Kamoshita Maki Kato Tsubasa Sagara Eri Hisamatsu S Sakaue M Sakuma Tetsushi Yamamoto Takashi Ito Junya Kashiwazaki Naomi

¹Graduate School of Veterinary Sciences, Azabu University, Kanagawa, Japan; ²School of Veterinary Medicine, Azabu University, Kanagawa, Japan; ³Faculty of Life and Environmental Sciences, Azabu University, Kanagawa, Japan; ⁴Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Hiroshima, Japan; ⁵Azabu University, Sagamihara-shi, Japan.

ra0001p078

Abstract unavailable

ra0001p079

Isolation and characterization of porcine mesenchymal stem cell as a donor for efficient nuclear transfer

Lee Jun Sung Jung Sung Han Park Young Bin Kim Sang Eun Lee Hoon Taek

Konkuk University, Seoul, Republic of Korea.

ra0001p080

Establishment of efficient system in the DNA microinjection into porcine in vitro embryos

Malaweera Don Buddika Oshadi Ramachandra Sisitha Kim Ghangyong Kim Seokjoong Cho Jongki

¹Chungnam National University, Daejeon, Republic of Korea; ²Toolgen, Seoul, Republic of Korea.

ra0001p081

The effect of development in vitro

Obata Hitomi Kamoshita Maki Kato Tsubasa Ito Junya Kashiwazaki Naomi

¹Graduate School of Veterinary Sciences, Azabu University, Kanagawa, Japan; ²Azabu University, Sagamihara-shi, Japan.

ra0001p082

Comparison of survivin gene expression between porcine SCNT and iSCNT-derived normal and arrested embryos

Choi Kyu Hee Kim Young Kyu Choi Seong Deok Shin Sang Tae Lee Hoon Taek

¹Konkuk University, Seoul, Republic of Korea; ²Chungnam National University, DaeJeon, Republic of Korea.

ra0001p083

Involvement of poly(ADP-ribosyl)ation and autophagy in porcine pre-implantation development

Lee Hye Ran Hwang Jeongho Park Hyo Chang Han Yong Man Lee Hoon Taek

¹Konkuk University, Seoul, Republic of Korea; ²KAIST, Daejeon, Republic of Korea.

ra0001p084

Transporters for L-proline in the pre-implantation mouse embryo

Day Margot L Zada M Bailey Charles Treleaven Tamara Ozsoy Sukran Rasko John EJ Morris Michael B

¹The University of Sydney, Sydney, Australia; ²Centenary Institute, Camperdown, Australia.

ra0001p085

Treatment of antioxidant (acteoside) in fetal fibroblats improves the efficiency of canine cell cloning

Lee Ji Hye Kim Keun Jung Kim Eun Young Xia Li Xiao Park Kang Sun Han Kil Woo Lee Jin Hee Lee Bo Myeong Yu Jeong Zhuang Li Li Kim Dong Hee Lee Kyung Bon Kim Dong-Hoon Kim Min Kyu

¹Chungnam National University, Daejeon, Republic of Korea; ²Chonnam National University, Gwangju, Republic of Korea; ³National Institute of Animal Science, Suwon, Republic of Korea.

ra0001p086

Semi-quantification and elucidation of bovine embryo biomarkers by mass spectrometry imaging

Goncalves Roseli F Ferreira Monica S de Oliveira Diogo N Canevarolo Rafael R Achilles Marcos A Killian Gary J Bols Peter E Visintin Jose A Catharino Rodrigo R

¹College of Veterinary Medicine and Animal Science, São Paulo, Brazil; ²University of Campinas, Campinas, Brazil; ³National Energy and Material Research Center, Campinas, Brazil; ⁴Achilles Genetics, Garça, Brazil; ⁵The Pennsylvania State University, State College, USA; ⁶University of Antwerp, Wilrijk, Belgium.

ra0001p087

Artificial activation enhances pre-implantation embryo development after intracytoplasmic injected with evaporative-dried sperm

Li Xiao Xia Lee Ji Hye Kim Keun Jung Kim Eun Young Park Kang Sun Han Kil Woo Lee Jin Hee Lee Bo Myeong Kim Dong Hee Yu Jeong Zhuang Li Li Kim Min Kyu

Chungnam National University, Daejeon, Republic of Korea.

ra0001p088

Construction of porcine FoxN1 knockout system by RNA-guided endonucleases

Park Jae-Kyung Jang Hoon Kim Donghwan Sun Wu Sheng Kim Hyoung-Joo Yeo Jaehun Hwang Seongsoo Lee Jeong-Woong

¹Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea; ²Rural Development Administration, Suwon-si, Republic of Korea; ³National Institute of Animal Science, RDA, Suwon, Republic of Korea.

ra0001p089

Expression pattern and function of claudins during tight junction maturation in the mouse pre-implantation embryo

Sheth Bhavwanti Cox Andrew L Greenslade Karen J Alexopoulos Orpheas Fleming Tom P

University of Southampton, Southampton, UK.

ra0001p090

Human granulocyte-colony stimulating factor enhances viability of porcine embryos in defined oocyte maturation medium

Cai Lian Hyun Sang-Hwan

College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea.

ra0001p091

Chromatin remodelling and histones mRNA accumulation in bovine germinal vesicle oocyte

Labrecque Remi Lodde Valentina Dieci Cecilia Tessaro Irene Luciano Alberto M Sirard Marc-Andre

¹Université Laval, Ville de Québec, Canada; ²University of Milan, Milan, Italy.

ra0001p092

Effect of ganglioside GT1b treatment during porcine in vitro maturation on embryonic development and mRNA expression pattern in cumulus cell

Hwang Seon-Ung Hyun Sang-Hwan

College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea.

ra0001p093

The antioxidative effect of carboxyethylgermaniumsesquioxide (Ge-132) on in vitro maturation of porcine oocytes and subsequent embryonic development after in vitro fertilization and parthenogenetic activation

Kim Eunhye Hyun Sang-Hwan

College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea.

ra0001p094

The effect of vitrification on subsequent mouse embryo development and postnatal health

Khalif Raja Ili Airina Raja Smyth Neil Fleming Tom Velazquez Miguel Abraham Sheth Bhav Smith Stephanie

University of Southampton, Southampton, UK.

ra0001p095

Insulin and branched-chain amino acid depletion during mouse in vitro preimplantation development alters postnatal growth and cardiovascular physiology

Velazquez Miguel Abraham Sheth Bhav Marfy-Smith Stephanie Eckert Judith Fleming Tom

University of Southampton, Southampton, UK

ra0001p096

Supplementation with sunflower seed increases conception rates in recipient beef heifers

Membrive Claudia Maria Bertan Cordeiro Mariangela Bueno de Souza Johnny Maciel Filho Manoel Francisco Sa Pugliesi Guilherme de Paula Nogueira Guilherme Santin Tiago Binelli Mario

¹UNESP – University of São Paulo State, Dracena, Brazil; ²UNESP – University of São Paulo State, Araçatuba, Brazil; ³USP – University of São Paulo, Pirassununga, Brazil.

ra0001p097

The role of vitamin C in in vitro early embryonic development: verification using aldehyde reductase gene knockout mice

Hoshino Yuki Watanabe Ren Takahashi Motoko Miyata Satoshi Yoshida Yasukazu Fujii Junichi Kimura Naoko

¹Graduate School of Agricultural Science, Yamagata University, Tsuruoka, Japan; ²Sapporo Medical University School of Medicine, Sapporo, Japan; ³Osaka Koseinenkin Hospital, Osaka, Japan; ⁴National institute of advanced industrial science and technology (AIST), Ikeda, Japan; ⁵Graduate School of Medical Science, Yamagata University, Yamagata, Japan.

ra0001p098

Selection of reference genes in mouse preimplantation embryos of different ploidies at various developmental stages

Gu Yanli Shen Xinghui Zhou Dongjie Wang Zhendong Lei Lei

Harbin Medical University, Harbin, China.

ra0001p099

Competence of porcine first polar body for normal development

Jin Dong Il Lin Tao Diao Yun Fei Won Kang Jung Kim Dong Kyo

Chungnam National University, Daejeon, Republic of Korea.

ra0001p100

Association between birth weight and age at menarche: a meta-analysis

Yermachenko Anna He Hao Dvornyk Volodymyr

¹The University of Hong Kong, Hong Kong; ²Department of Biostatistics and Bioinformatics, Tulane University, New Orlean, Louisiana, USA.

ra0001p101

Effect of downregulating GLIS1 transcript on early development and gene expression of bovine embryos

Takahashi Kazuki Sakurai Nobuyuki Hashizume Tsutomu Sawai Ken

Iwate University, Morioka, Japan.

ra0001p102

Genome-wide reprogramming by DNA demethylation during mouse oocyte growth and early development

Sakashita Akihiko Iseki Yosuke Nakajima Mei Wakai Takuya Kobayashi Hisato Kono Tomohiro

Tokyo University of Agriculture, Tokyo, Japan.

ra0001p103

Expression profile of imprinted genes and DNA methyl transferases in uniparental sheep placenta

Zacchini Federica Toschi Paola Czernik Marta Loi Pasqualino Ptak Grazyna Ewa

University of Teramo, Teramo, Italy.

ra0001p104

Genome editing technology using ZFN and TALEN is effective on cultured porcine ST cells knockout pig

Kato Tsubasa Sakaue M Hisamatsu S Kamoshita M Obata H Sakuma Tetsushi Yamamoto Takashi

¹Graduate School of Veterinary Sciences, Azabu University, Kanagawa, Japan; ²School of Veterinary Medicine, Azabu University, Kanagawa, Japan; ³Faculty of Life and Environmental Sciences, Azabu University, Kanagawa, Japan; ⁴Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Hiroshima, Japan.

ra0001p105

Genome-wide DNA methylation analysis of bovine clones derived from the same donor cells

Kaneda Masahiro Watanabe Shinya Hirako Makoto Akagi Satoshi Kiefer Helene Jouneau Luc Martin-Magniette Marie-Laure Balzergue Sandrine Nagai Takashi

¹Tokyo University of Agriculture and Technology, Fuchu, Japan; ²NARO Institute of Livestock and Grassland Science, Tsukuba, Japan; ³INRA, Jouy-en-Josas, France; ⁴INRA, Paris, France; ⁵INRA, Evry, France; ⁶Food and Fertilizer Technology Center, Taipei, Taiwan

ra0001p106

DNA methylation analysis in sperm from boars exhibiting normal and altered spermograms

Congras Annabelle Yerle-Bouissou Martine Vignoles Florence Lhuillier Emeline Foissac Sylvain Pinton Alain Ferchaud Stephane Bouchez Olivier Riquet Juliette Acloque Herve

¹INRA, Castanet-Tolosan, France; ²ENVT, Toulouse, France; ³INRA, Surgères, France.

ra0001p107

Effect of the way in which ewes were handled during mid-gestation on testicular and ovarian development in their lambs

de Jonge Suzanne Hogg Charis O. Baxter Emma Hall Sarah Dwyer Cathy M. Ashworth Cheryl Joy

¹University of Edinburgh, Edinburgh, UK; ²Scotland’s Rural College, Edinburgh, UK

ra0001p108

Postovulatory ageing influences methylation marks and development in bovine oocytes and early embryos

Heinzmann Julia Mattern Felix Aldag Patrick Lucas-Hahn Andrea Wrenzycki Christine Haaf Thomas Niemann Heiner

¹Institute of Farm Animal Genetics (FLI), Neustadt, Germany; ²Julius-Maximilians-University, Wuerzburg, Germany; ³Justus-Liebig-University, Giessen, Germany.

ra0001p109

Epigenetic regulation of Gata-6 transcription factor expression in mouse primitive endoderm extra-embryonic lineage induced by maternal protein restricted diet

Sun Congshan Sheth Bhav Smyth Neil Cox Andrew L. Denisenko Oleg Fleming Tom

¹Randall division of cell and molecular biophysics, London, UK; ²University of Southampton, Southampton, UK; ³University of Washington, Seattle, USA.

ra0001p110

Lactation litter size differentially affects satiety hormone concentrations and gut adaptations in Wistar rat dams

Johnson Michelle L Saffrey M. Jill Taylor Vicky J.

The Open University, Milton Keynes, UK.

ra0001p111

TAF4b promotes oocyte survival and proper ovarian chromatin state in the mouse

Grive Kathryn Jennifer Ribiero Jennifer R. Seymour Kimberly A. Freiman Richard N.

Brown University, Providence, USA.

ra0001p112

Combinations of 17[beta]-estradiol and androgen support bovine oocyte growth in vitro by maintaining physical connections with granulosa cells

Makita Miho Miyano Takashi

Kobe University, Kobe, Japan.

ra0001p113

Anti-Mullerian Hormone (AMH) regulates the growth and maturation of primate antral follicles during the menstrual cycle

Xu Jing Lawson Maralee S. Bishop Cecily V.

Oregon Health & Science University, Beaverton, Oregon, USA.

ra0001p114

Critical role of decoy receptor 3 in regulation of granulosa cell apoptosis in pig ovarian follicle

Fukumoto Yoshinosuke Sugimoto Miki Onoyama Ichiro Wongpanit Kannika Manabe Noboru

¹The University of Tokyo, Kasama, Japan; ²Kyoto University, Kyoto, Japan; ³Kasetsart University, Sakon Nakhon, Thailand.

ra0001p115

Expression and function of lysophosphatidic acid in theca cells of the bovine ovarian follicle

Sinderewicz Emilia Boruszewska Dorota Kowalczyk-Zieba Ilona Staszkiewicz Joanna Grycmacher Katarzyna Woclawek-Potocka Izabela

Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland.

ra0001p116

Expression and function of lysophosphatidic acid in granulosa cells of the bovine ovarian follicle

Woclawek-Potocka Izabela Sinderewicz Emilia Boruszewska Dorota Kowalczyk-Zieba Ilona Staszkiewicz Joanna Grycmacher Katarzyna

Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland.

ra0001p117

Evaluation of oocyte quality and ovarian function in aged female cystine-glutamate transporter gene-deficient mice

Watanabe Ren Fujii Junichi Sato Hideyo Kimura Naoko

¹Graduate School of Agricultural Science, Yamagata University, Tsuruoka, Japan; ²Graduate School of Medical Science, Yamagata University, Yamagata, Japan.

ra0001p118

Ovarian and hormonal responses of female goats to feed restriction and intermittent administration of neurokinin B analog

Endo Natsumi Tanaka Tomomi

Tokyo University of Agriculture and Technology, Fuchu, Japan.

ra0001p119

Differential changes in LH secretion after administration of investigational metastin/kisspeptin analog TAK-683 to goats

Tamesaki Chie Endo Natsumi Ohkura Satoshi Wakabayashi Yoshihiro Matsui Hisanori Tanaka Akira Watanabe Tatsuya Okamura Hiroaki Tanaka Tomomi

¹Tokyo University of Agriculture and Technology, Fuchu, Japan; ²Nagoya University, Nagoya, Japan; ³National Institute of Agrobiological Sciences, Tsukuba, Japan; ⁴Takeda Pharmaceutical Company, Fujisawa, Japan.

ra0001p120

Endocrine responses and ovarian dynamics in goats treated with low dose of investigational metastin/kisspeptin analog TAK-683 in follicular phase

Gomi Nahoko Endo Natsumi Ohkura Satoshi Wakabayashi Yoshihiro Matsui Hisanori Matsumoto Hirokazu Ishikawa Kaori Tanaka Akira Watanabe Tatsuya Okamura Hiroaki Tanaka Tomomi

¹Tokyo Univesity of Agriculture and Technology, Fuchu, Japan; ²Nagoya University, Nagoya, Japan; ³National Institute of Agrobiological Sciences, Tsukuba, Japan; ⁴Takeda Pharmaceutical Company, Fujisawa, Japan.

ra0001p121

TLR4-mediated signaling pathway is modulated by melatonin through MyD88-dependent pathway in ovarian carcinoma of ethanol-consuming rats

Chuffa Luiz Gustavo Fioruci-Fontanelli Beatriz Mendes Leonardo Pinheiro Patricia Fernanda Martinez Marcelo Martinez FranciscoEduardo

¹Botucatu, Brazil; ²Ufscar, São Carlos, Brazil.

ra0001p122

Relative transcript abundance in porcine cumulus cells collected from different size follicles

Nicolas Carla Moros Rico Maria Jose Izquierdo Li Y Romar Rakel Funahashi H

¹University of Murcia, Murcia, Spain; ²Okayama University, Okayama, Japan.

ra0001p123

Real-time observation of hormone-secretion using CFP, YFP-fused gonadotropin expression vector

Takanashi Haruka Nishimura Naoto Yoshida Saishu Higuchi Masashi Kawai Kohei Ueharu Hiroki Kato Takako Kato Yukio

¹Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa, Japan; ²Meiji University, Kanagawa, Japan; ³Department of Life Science, School of Agriculture, Meiji University, Kanagawa, Japan.

ra0001p124

BMP15 plus GDF9 and cAMP modulator promote EGF receptor signaling to increase oocyte developmental competence in porcine cumulus-oocyte complexes from small follicles

Sugimura Satoshi Ritter Lesley J Rose Ryan D Thompson Jeremy G Mottershead David G Gilchrist Robert B

¹Tokyo University of Agriculture and Technology, Fuchu, Japan; ²Robinson Research Institute, The University of Adelaide, Adelaide, South Australia, Australia; ³The University of Adelaide, Adelaide, South Australia, Australia; ⁴University of New South Wales, Randwick, New South Wales, Australia.

ra0001p125

TGF[beta] regulation of the human fetal ovarian matrix

Bayne Rosemary A L Roussi Kalliopi Childs Andrew Rodgers Ray Anderson Richard

¹University of Edinburgh, Edinburgh, UK; ²The Royal Veterinary College, London, UK; ³Adelaide University, Adelaide, South Australia, Australia.

ra0001p126

Abstract unavailable

ra0001p127

Effects of the mycotoxin, deoxynivalenol, on bovine granulosa cells

Guerrero-Netro Hilda Morayma Chorfi Younes Price Christopher A

Université de Montreal, Saint-Hyacinthe, Quebec, Canada

ra0001p128

Transcriptome in small antral follicles of monkeys on a western-style diet with/without testosterone

Xu Fuhua Xu Jing Bishop Cecily V Cameron Judy L Stouffer Richard L

¹Oregon National Primate Research Center, OHSU, Beaverton, Oregon, USA; ²Oregon Health & Science University, Beaverton, Oregon, USA; ³University of Pittsburgh, Pittsburgh, Pennsylvania, USA.

ra0001p129

The effects of estradiol and ECG combination on ZP3 gene in mouse oocyte

Mohammadzadeh Masoomeh Shahbazi Majid Haidari Kamran

Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran.

ra0001p130

Inhibin [alpha] induced porcine granulosa cells apoptosis through mitochondrial apoptotic pathway

Li Wan-Hong Chen Shu-Xiong Chen Lu Li Chunjin Zhou Xu

Jilin University, Changchun, China.

ra0001p131

Abstract unavailable

ra0001p132

The effect of bisphenol A on Notch signaling pathway in the follicular development of neonatal rat ovary

Altunbas Korhan Celik Sefa Yagci Artay Akkaya Ozlem Ozden

Afyon Kocatepe University, Afyonkarahisar, Turkey.

ra0001p133

Abstract unavailable

ra0001p134

Interaction between galectin-1 and glycoconjugates is involved in hCG-mediated gene expression in cultured human luteinized granulosa cells

Nio-Kobayashi Junko Iwanaga Toshihiko Duncan W Colin

¹Laboratory of Histology and Cytology, Hokkaido University Graduate School of Medicine, Sapporo, Japan; ²The Queen’s Medical Research Institute, Centre for Reproductive Health, The University of Edinburgh, Edinburgh, UK.

ra0001p135

Expression of steriod hormone receptor in the ovary and oviduct during the canine estrus cycle

Yang Byoung-Chul Yoo Jae Gyu Kim Dong-Hoon Kim Dong-Kyo Kim Hyun-Min No Jin-Gu Yeom Dong-Hyeon Choi Mi-Kyung Nam Yoon-Seok

National Institute of Animal Science, Rural Development Administration, Suwon, Republic of Korea.

ra0001p136

Effect of progesterone levels and parity on the maturity of in vivo canine oocytes

Kim Dong-Hoon Choi Mi-Kyung No Jin-Gu Yeom Dong-Hyeon Kim Hyun-Min Kim Dong-Kyo Yang Byoung-Chul Yoo Jae Gyu

National Institute of Animal Science, Rural Development Administration, Suwon, Republic of Korea.

ra0001p137

The development of a novel mouse embryonic ovary culture

Stefansdottir Agnes Adams Ian Spears Norah

University of Edinburgh, Edinburgh, UK.

ra0001p138

Regulatory role of resistin on ovarian function

Rak-Mardyla Agnieszka Wrobel Anna Drwal Eliza Gregoraszczuk Ewa

Jagiellonian University in Krakow, Krakow, Poland.

ra0001p139

The effects of bisphenol A in in vitro neonatal rat ovary

Altunbas Korhan Yagci Artay Celik Sefa Akkaya Ozlem Ozden Zik Berrin

¹Afyon Kocatepe University, Afyonkarahisar, Turkey; ²Deparment of Histology and Embryology, Faculty of Veterinary, Uludag University, Nilüfer BURSA, Bursa, Turkey.

ra0001p140

Acquisition of meiotic competence in porcine secondary follicles following long-term culture

Tasaki Hidetaka Itami Nobuhiko Iwata Hisataka

Tokyo University of Agriculture, Atsugi, Japan.

ra0001p141

Isolation of primordial and primary follicles from porcine ovarian medulla tissue

Duda Malgorzata Grzesiak Malgorzata Tabarowski Zbigniew Tomanek Milan

¹Jagiellonian University, Krakow, Poland; ²Institute of Animal Science, Prague, Uhříněves, Czech Republic.

ra0001p142

Analysis of programmed cell death of porcine granulosa cells treated with vinclozolin

Slomczynska Maria Knet Malgorzata Tabarowski Zbigniew Duda Malgorzata

Jagiellonian University, Krakow, Poland.

ra0001p143

Etoposide has a detrimental impact on mouse ovarian development when exposure occurs during early meiotic prophase

Johnston Zoe C Stefansdottir Agnes Adams Ian Spears Norah

¹Centre for Integrative Physiology, University of Edinburgh, Edinburgh, UK; ²MRC Human Genetics Unit MRC IGMM, University of Edinburgh, Edinburgh, UK.

ra0001p144

The age-associated deterioration in the follicular fluid induces a decline in bovine oocyte quality

Takeo Shun Kuwayama Takehito Iwata Hisataka

Tokyo University of Agriculture, Atsugi, Japan.

ra0001p145

Resveratrol enhances the in vitro development of oocytes derived from early antral follicles

Itami Nobuhiko Kuwayama Takehito Iwata Hisataka

Tokyo University of Agriculture, Atsugi, Japan.

ra0001p146

Expression of pentraxin 3 transcript and protein in porcine preovulatory follicles: comparison of in vivo and in vitro conditions

Nagyova Eva Nemcova Lucie Kalous Jaroslav Salustri Antonietta Camaioni Antonella

¹Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov, Czech Republic; ²University of Rome Tor Vergata, Rome, Italy.

ra0001p147

Cell viability in ovarian follicles exposed to cisplatin and doxorubicin

Kirkwood Phoebe Maud Lopes Federica Spears Norah

The University of Edinburgh, Edinburgh, UK.

ra0001p148

Ghrelin, like adipokines produced by adipose tissue, acts on accelerate puberty, while in contrast to adipokines, during sexual maturity prevents from excessive secretion of steroids by ovarian follicles

Rak-Mardyla Agnieszka Wrobel Anna Gregoraszczuk Ewa

Jagiellonian University in Krakow, Krakow, Poland.

ra0001p149

Abstract unavailable

ra0001p150

Detection of ZP-autoantibody in the sera from infertile women using a human multiepitope peptide as antigen

Xu Wan Xiang Wang Jian Zhu Weijie Chang Xiaoxue Sun Xiaoxi Gupta Satish K He Yaping Tang Haiping Shi Huijuan Gu Shaohua Ji Chaoneng Xie Yi

¹Shanghai Institute of Planned Parenthood Research, Shanghai, China; ²Institute of Reproductive Immunology, Jinan University, Guangzhou, China; ³Medical School, Henan University of Science and Technology, Luoyang, China; ⁴Shanghai Medical College, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China; ⁵National Institute of Immunology, New Delhi, India; ⁶School of Life Science, Institute of Genetics, Fudan University, Shanghai, China; ⁷State Key Laboratory of Genetic Engineering, School of Life Sciences, Institute of Genetics, Fudan University, Shanghai 200433, People’s Republic of China.

ra0001p151

Occurrence of rare three types of chromosome configurations in a Murrah buffalo (Bubalus Bubalis)

Sen Sulochana Tonk Rakesh Yadav B R

National Dairy Research Institute, Karnal, India.

ra0001p152

Effect of different diet fat sources on in vitro maturation of Anglo-Nubian goats oocytes

Nociti Ricardo P Padilha Luciana C Rola Luciana D Barros Felipe F F P C Uscategui Ricardo R Mariano Renata S G Feliciano Marcus A R Vicente Wilter R R Oliveira Maria Emilia F Lima Vera F M H

FCAV/UNESP, Jaboticabal, Brazil.

ra0001p153

Chronic sympathetic stress during gestation delays puberty and follicular development of female offspring

Barra Rafael Cruz Gonzalo Mayerhofer Artur Paredes Alfonso Lara Hernan E

¹Faculty of Chemistry and Pharmaceutical Sciences, Santiago, Chile; ²Facultad de Ciencias, Valparaíso, Chile; ³University of Munich, Munich, Federal Republic of Germany.

ra0001p154

Proteins in porcine follicular fluid as potential biomarkers for fertility

Jarrett Selene Gill Andy C Kurian Dominic Hogg Charis O Ferguson Elizabeth M Ashworth Cheryl Joy

¹University of Edinburgh, Edinburgh, UK; ²The Roslin Institute, Edinburgh, UK; ³Aberdeen Maternity Hospital, Aberdeen, UK.

ra0001p155

Glucocorticoid metabolism in bovine cumulus-oocyte complex during in vitro maturation

Tetsuka Masafumi Takagi Ryo Ambo Nobuhiro Zempo Yuta Onuma Asuka

Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.

ra0001p156

A high concentration of polyvinylpyrrolidone in culture medium affects the number of tranzonal projections in bovine oocyte-granulosa cell complexes in long-term culture

Hirao Yuji Somfai Tamas Akagi Satoshi Haraguchi Seiki Watanabe Shinya

NARO Institute of Livestock and Grassland Science, Tsukuba, Japan

ra0001p157

Mouse CD11c+ dendritic cells are crucial in folliculogenesis, luteinization, and luteal maintenance

Nagai Miwako Koga Kaori

¹Toranomon Hospital, Minato-ku, Japan; ²The University of Tokyo, Bunkyo, Japan.

ra0001p158

Investigating whether Kiss1 KO mice can be used as a model for PCOS and age-onset diabetes

Kyle Victoria d'Anglemont de Tassigny Xavier Colledge William H

University of Cambridge, Cambridge, UK.

ra0001p159

Androgen action within the preantral follicle; a role for the epidermal growth factor receptor?

Thomson Kacie Laird Mhairi Franks Stephen Hardy Kate

Imperial College London, London, UK.

ra0001p160

Differential role of TGF[beta]2 during follicle activation and preantral follicle growth

Oliver Elizabeth Laird Mhairi Franks Stephen Hardy Kate

Imperial College London, London, UK.

ra0001p161

Mycotoxin-contaminated diet affects mouse oocyte quality

Hou Yan-Jun Zhu Cheng-Cheng Cui Xiang-Shun Kim Nam-Hyung Sun Shao-Chen

¹Nanjing Agricultural University, Nanjing, China; ²Chungbuk National University, Cheongju, Republic of Korea.

ra0001p162

Abstract unavailable

ra0001p163

Obesity depresses Toll-like receptor superfamily members in the ovary

Galvao Antonio Socha Barbara Jura Magdalena Nowialis Pawel Skarzynski Dariusz

Institute of Animal Reproduction and Food Research, Polish Academy of Science, Olsztyn, Poland.

ra0001p164

Space pup project: sperm preservation in space station

Wakayama Sayaka Yano Sachiko Kasahara Haruo Osada Ikuko Shimizu Toru Suzui Hiromi Mizutani Eiji

¹Yamanashi University, Kofu City, Japan; ²JAXA, Tukuba City, Japan; ³JAMSS, Tukuba City, Japan; ⁴JSF, Tukuba City, Japan.

ra0001p165

Amino acid and glucose profiles of bovine oviduct epithelial cell secretions in response to 17[beta]-oestradiol and progesterone

Simintiras Constantine A Sathyapalan Thozhukat Leese Henry J Sturmey Roger

Hull York Medical School, Kingston upon Hull, UK.

ra0001p166

Generation of rats from vitrified oocytes with surrounding cumulus cells via IVF with cryopreserved sperm

Kashiwazaki Naomi Fujiwara Katsuyoshi Kato Tsubasa Kamoshita M Obata H Takakusa Syouta Ito Junya

¹Azabu university, Sagamihara-shi, Japan; ²Graduate School of Veterinary Sciences, Azabu University, Kanagawa, Japan.

ra0001p167

Comparative effects of administration of kisspeptin-10 and GnRH on LH secretion in buffalo cows

Chaikhun-Marcou Thuchadaporn Sotthibandhu Pongsiwa Suthikrai Wanwipa Jintana Ratree Makoom Pussadee Suadsong Siriwat

¹Obstetrics Gynecology and Reproduction, Veterinary Science, Chulalongkorn University, Bangkok, Thailand; ²Obstetrics Gynecology and Animal Reproduction Clinic, Veterinary Medicine, Mahanakorn University of Technology, Bangkok, Thailand; ³Pre-Clinical Veterinary Science, Veterinary Medicine, Mahanakorn University of Technology, Bangkok, Thailand; ⁴Research and Development Centre for Livestock Production Technology, Chulalongkorn University, Bangkok, Thailand; ⁵Veterinary Diagnostic Center, Veterinary Science, Chulalongkorn University, Bangkok, Thailand.

ra0001p168

Ovulation induction in prepubertal Sahiwal[times]Friesian cross bred heifers by the use of clomiphene citrate and hCG

-ur-Rehman Ubaid Ali Shujait Ahmad Nazir Khanum Shahnaz Adeeb

¹Department of Theriogenology, University of Agriculture, Faisalabad, Pakistan; ²Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan.

ra0001p169

Abstract unavailable

ra0001p170

Evaluation of the cyclophosphamide gonadotoxicity and the protective effect of GnRH analogues in mice model

Horicks Florence Bockstaele Laurence Houben Sarah Van den Steen Geraldine Englert Yvon Demeestere Isabelle

Université Libre de Bruxelles, Brussels, Belgium.

ra0001p171

Abstract unavailable

ra0001p172

Fibrotic remodeling and duct obstruction are major patho-physiological mechanisms in bacterial epididymitis in mice

Michel Vera Bhushan Sudhanshu Middendorff Ralf Loveland Kate L de Kretser David D Hedger Mark P Meinhardt Andreas

¹Justus Liebig University Giessen, Germany; ²Monash University, Clayton, Victoria, Australia

ra0001p173

Model for differentiation of mouse embryonic stem cells into steroidogenic cells

Kang Hee Young Lee Jae-Hwan Jeung Eui-Bae

Chungbuk National University, Cheongju, Republic of Korea.

ra0001p174

Early life programming of adult Leydig cell function

Kilcoyne Karen R Smith Lee Jobling Matthew McKinnell Chris MacPherson Sheila van den Driesche Sander De Gendt Karel Verhoeven Guido Anderson Richard Sharpe Richard

¹University of Edinburgh, Edinburgh, UK; ²Catholic University of Leuven, Leuven, Belgium; ³Legendo, Leuven, Belgium.

ra0001p175

Characterization of pituitary-derived cell lines, Tpit/E, Tpit/F1 and TtT/GF

Nishimura Naoto Yoshida Saishu Higuchi Masashi Yako Hideji Ueharu Hiroki Chen Mo Kato Takako Kato Yukio

Meiji University, Kanagawa, Japan.

ra0001p176

Functional and molecular features of the ID4+ germline stem cell population in mouse testes

Oatley Melissa Chan Frieda Kaucher Amy Oatley Jon

Washington State University, Pullman, Washington, USA.

ra0001p177

Presence of the juxtacrine factor EphrinB2 in a rat pituitary stem/progenitor cell niche

Yoshida Saishu Kawai Kohei Kato Takako Kato Yukio

¹Meiji University, Kanagawa, Japan; ²Department of Life Science, School of Agriculture, Meiji University, Kanagawa, Japan.

ra0001p178

Kruppel-like factor 6 (klf6) is expressed in rat pituitary stem/progenitor cells and regulates the PRRX2 gene

Ueharu Hiroki Higuchi Masashi Nishimura Naoto Yoshida Saishu Nishihara Hiroto Kato Takako Kato Yukio

Meiji University, Kanagawa, Japan.

ra0001p179

Concentration levels of TSH evaluation with species-specific MABs due to the dog reproduction disorders assessment in the course of hypothyroidism

Aniolek Olga Gajewski Zdzislaw Domino Malgorzata

Warsaw University of Life Scienses, Warsaw, Poland.

ra0001p180

Invasion of S100[beta]-positive cells into pituitary gland during embryonic period

Yako Hideji Horiguchi Kotaro Higuchi Masashi Fujiwara Ken Yoshida Saishu Chen Mo Naoko Kanno Ueharu Hiroki Kato Takako Yashiro Takashi Kato Yukio

¹Division of Life Science, Graduate School of Agriculture, Kawasaki, Japan; ²Department of Health Sciences, Kyorin University, Japan; ³Meiji University, Kanagawa, Japan; ⁴Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine, Japan.

ra0001p181

Neuronatin is first expressed in pituitary stem/progenitor cells

Naoko Kanno Higuchi Masashi Yako Hideji Yoshida Saishu Kato Takako Chen Mo Kato Yukio

Meiji University, Kanagawa, Japan.

ra0001p182

Early aging of mesenchymal stem/stromal cells derived from the bone marrow of [alpha]-1,3-galactosyltransferase knockout pig

Ock Sun A Oh Keon Bong Hwang Seongsoo Im Seo Ki Kim Youngim

National Institute of Animal Science, RDA, Suwon, Republic of Korea.

ra0001p183

Derivation of naive-type induced pluripotent stem cells in cattle using piggyBac transposition of doxycycline-inducible transcription factors

Kawaguchi Takamasa Tsukiyama Tomoyuki Minami Naojiro Yamada Masayasu Matsuyama Shuichi Kimura Koji Imai Hiroshi

¹Kyoto University, Kyoto, Japan; ²RIKEN Center for Developmental Biology (CDB), Kobe, Japan; ³National Institute of Livestock and Grassland Science, Nasushiobara, Japan.

ra0001p184

Serum-free culture of mouse trophoblast stem cells

Sun Shuai Yano Shota Nakanishi Momo O Hirose Michiko Nakabayashi Kazuhiko Hata Kenichiro Ogura Atsuo Shiota Kunio Tanaka Satoshi

¹The University of Tokyo, Bunkyo-ku, Japan; ²Developmental and Stem Cell Biology Hospital for Sick Children, Toronto, Ontario, Canada; ³RIKEN BRC, University of Tsukuba, Tsukuba, Japan; ⁴National Research Institute for Child Health and Development, Setagaya-ku, Japan.

ra0001p185

Incomplete reprogramming of bovine fetal fibroblasts by the stimulus of low pH*

Jin Sang Jin Kim Tae Suk Baek Sang Ki Moon Song Yi Ko Yeoung-Gyu Kim Sung Woo Seong Hwan Hoo Lee Joon Hee

¹Animal Development and Biotechnology Group, Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University, Jin ju, Republic of Korea; ²Institute of Agriculture and Life Science, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 660-701, Republic of Korea; ³Animal Genetic Resources Station, Namwon, Republic of Korea; ⁴National Institute of Animal Science, Rural Development Administration, kwonsun-Gu, Republic of Korea.

ra0001p186

Leukemia inhibitor factor (LIF) is essential for long term maintenance of pluripotency of porcine induced pluripotent stem cells*

Baek Sang Ki Kim Tae Suk Moon Song Yi Jin Sang Jin Ko Yeoung Gyu Kim Sung Woo Seong Hwan Hoo Lee Joon Hee

¹Animal Development and Biotechnology Group, Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University, Jin ju, Republic of Korea; ²Institute of Agriculture and Life Science, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 660-701, Republic of Korea; ³Animal Development and Biotechnology Group, Department of Animal Bioscience, Jin ju, Republic of Korea; ⁴Animal Genetic Resources Station, National Institute of Animal Science, Namwon, Republic of Korea; ⁵National Institute of Animal Science, Rural Development Administration, kwonsun-Gu, Republic of Korea.

ra0001p187

Function of leukemia inhibitory factor in spermatogenesis, revealed by using medaka spermatogonial culture system

Satoh Ryuichi Yamashita Masakane

Hokkaido University, Sapporo, Japan.

ra0001p188

RNA binding protein Lin28a interacts with various mRNA in the mouse trophoblast stem cells

Sakamaki Yuji Hayakawa Koji Tanaka Satoshi

The University of Tokyo, Bunkyo-ku, Japan.

ra0001p189

Derivation of porcine iPS-like cells from fibroblast of a translocated azoospermic boar

Congras Annabelle Barasc Harmonie Vignoles Florence Delcros Chantal Pinton Alain Canale-Tabet Kamila Feraud Olivier Turhan Ali Yerle-Bouissou Martine Acloque Herve

¹INRA, Castanet-Tolosan, France; ²ENVT, Toulouse, France; ³INSERM, Villejuif, France; ⁴INSERM, Poitiers, France.

ra0001p190

Derivation of bovine induced pluripotent stem cells by a transposon approach

Rao Talluri Thirumala Kumar Dharmendra Glage Silke Garrels Wiebke Debowski Katharina Behr Rudiger Niemann Heiner Kues Wilfried

¹Institute of Farm Animal Genetics, Neustadt am rbge, Germany; ²Central Institute for Research on Buffaloes, Hisar, India; ³Medical School Hannover, Hannover, Germany; ⁴German Primate Center, Göttingen, Germany.

ra0001p191

Effect of melatonin supplementation during in vitro maturation on intracellular ROS levels in porcine oocytes

Cruz Maria Helena Coelho Cruz Jurandir Ferreira Reiter Russel J Schwarz Katia Lancellotti Castro Fernanda Cavallari Cunha Maria Carolina R V Verde Leal Claudia Lima

¹University of São Paulo, Pirassununga, Brazil; ²Southwest Bahia State University, Vitória da Conquista/Bahia, Brazil; ³University Texas Health Science Center at San Antonio, San Antonio, Texas, USA.

ra0001p192

X chromosome dosage affects completion of pluripotential reprogramming

Kim Jong Soo Choi Hyun Woo Hong Yean Ju Scholer Hans R Do Jeong Tae

¹Konkuk University, Seoul, Republic of Korea; ²MPI for Molecular Biomedicine, Muenster, Germany.

ra0001p193

High-throughput screening revealed a clinically relevant drug to induce sperm motility

Cochrane Clair Ruso Halil Hope Anthony Clarke Rosemary G Barratt Christopher da Silva Sarah Martins

University of Dundee, Dundee, UK.

ra0001p194

Steps forward canine primordial germ cell isolation and characterization

de Souza Aline Fernanda Pieri Naira Caroline Godoy Cassals Juliana Barbosa Mancanares Ana Carolina Furlanetto Roballo Kelly Cristine Santos Ambrosio Carlos Eduardo Perecin Felipe Martins Daniele Santos

¹University of São Paulo, Pirassununga, Brazil; ²School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil.

ra0001p195

Effects of early embryo environment on embryonic stem cell derivation and behaviour

Cox Andrew L Sun Congshan Sheth Bhav Fleming Tom Smyth Neil

University of Southampton, Southampton, UK.

ra0001p196

Trophectoderm stem cells to model the effect of altered periconceptional diet on embryos

Chang Yi-Lung Fleming Tom Smyth Neil

University of Southampton, Southampton, UK.

ra0001p197

Effect of storage time on Atlantic salmon (Salmo salar) sperm motility, viability and calcium levels, first communication

Parodi Jorge

Universidad Catolica de Temuco, Temuco, Chile.

ra0001p198

Motility, viability and calcium in the sperm cells

Parodi Jorge

Universidad Catolica de Temuco, Temuco, Chile.

ra0001p199

Reciprocity of bio-stimulation on testicular traits and subsequent fertility of South African Mukota sows

Umesiobi Dennis Onuoha

Central University of Technology, Free State, Bloemfontein, South Africa.

ra0001p200

Alternative splicing: a mechanism for spermatogonia differentiation, meiosis progression and spermatid maturation

Jamsai Duangporn Clark Brett McLaughlin Eileen O'Bryan Moira

^aMonash University, Clayton, Victoria Australia; ^bUniversity of Newcastle, Newcastle, New South Wales, Australia.

ra0001p201

Different roads to fertility: distinct molecular and physiological strategies warrant the phenotype 'high fertility' in two outbred mouse models

Weitzel Joachim Sobczak Alexander Michaelis Marten

FBN Dummerstorf, Dummerstorf, Federal Republic of Germany.

ra0001p202

Abstract unavailable

ra0001p203

Recovery of porcine sperm from fetal testicular tissue xenografted into nude mice

Kikuchi Kazuhiro Noguchi Junko Kaneko Hiroyuki

National Institute of Agrobiological Sciences, Tsukuba, Japan.

ra0001p204

The impact of oxidative stress on chaperone-mediated human sperm-egg interaction

Grace Bromfield Elizabeth Nixon Brett Redgrove Kate Aitken Robert John

University of Newcastle, Callaghan, New South Wales, Australia.

ra0001p205

Prostaglandin J2 added to artificial insemination sperm dosage reduces the expression of the inflammatory genes in uterus sow

Izquierdo Rico Maria Jose Ross J W Soriano-Ubeda C Hernandez-Caravaca I Vieira L Matas C Garcia-Vazquez F A

¹University Of Murcia, Murcia, Spain; ²Iowa State University, Ames, Iowa, USA.

ra0001p206

Effect of extracellular long-chain fatty acids on mouse sperm motility

Moriyama Ryutaro Wakasa Ikumi Nakagawa Masako

Kinki University, Higashiosaka, Japan.

ra0001p207

Peroxisomes are essential for regular spermatogenesis

Brauns Ann-Kristin Kamalyan Lilit Baumgart-Vogt Eveline Luers Georg H

¹UKE, Hamburg, Germany; ²Justus-Liebig Universität, Giessen, Germany.

ra0001p208

Effect of local treatment of seminal vesiculitis on the quality of equine frozen semen

Sancler-Silva Yame F R Monteiro Gabriel A Neto Carlos Ramires Freitas Dell'aqua Camila P Crespilho Andre M Papa Frederico O Oliveira Pedro V

UNESP-BOTUCATU, Botucatu, Brazil.

ra0001p209

Proteomic analysis of human testicular interstitial fluid reflects disordered spermatogenesis in Klinefelter's syndrome

McLachlan Robert I Stephens Andrew N Rainczuk Adam Foo Caroline Condina Mark R Ishikawa Tomomoto Weidner Wolfgang Stanton Peter G

¹MIMIR-PHI Institute, Clayton, Victoria, Australia; ²Bruker Pty. Ltd, Melbourne, Victoria, Australia; ³Ishikawa Hospital, Himeji, Japan; ⁴Justus Liebig University, Giessen, Germany.

ra0001p210

The role of the molecular chaperone HSPA2 in unexplained failure of IVF

Nixon Brett Bromfield Elizabeth Redgrove Kate Baker Mark A Aitken Robert John

¹University of Newcastle, Callaghan, New South Wales, Australia; ²University of Newcastle, Newcastle, New South Wales, Australia.

ra0001p211

Androgen receptor signalling in testicular Leydig cells is essential for Leydig cell maturation and survival

O'Hara Laura McInnes Kerry Simitsidellis Ioannis Morgan Steph Milne Laura Mitchell Rod Smith Lee

¹MRC/University of Edinburgh Centre for Reproductive Health, Edinburgh, UK; ²University of Edinburgh/British Heart Foundation Centre for Cardiovascular Science, Edinburgh, UK; ³University of Edinburgh, Edinburgh, UK.

ra0001p212

Semen collection and preservation in the salt water crocodile Crocodylus porosus

Johnston Stephen Douglas

The University of Queensland, Gatton, Queensland, Australia.

ra0001p213

The association between spatial distribution patterns of SPACA1 in human ejaculated sperms and outcomes of conventional IVF

Kishida Kazumi Harayama Hiroshi Kimura Fuminori Murakami Takashi

¹School of Agricultural Science, Kobe University, Kobe City, Japan; ²Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu City, Japan.

ra0001p214

Detection of potassium channel defects in ICSI patients by patch clamp electrophysiology

Brown Sean G Mansell Steven Williams Hannah da Silva Sarah Martins Publicover Stephen Wilson Stuart Barratt Christopher

¹Abertay University, Dundee, UK; ²University of Dundee, Dundee, UK; ³University of Birmingham, Birmingham, UK; ⁴Durham University, Durham, UK.

ra0001p215

Spermatogenesis in pubertal common eland (Taurotragus oryx, Pallas 1766)

Pintus Eliana Ros-Santaella Jose Luis Kotrba Radim

Czech University of Life Sciences, Prague, Czech Republic.

ra0001p216

Evaluation of the binding capacity of fresh and frozen semen to the oviductal cells cultured in vitro

Carneiro Joao Alexandre Matos

Universidade Estadual Paulista ‘Julio de Mesquita Filho’, Botucatu, Brazil.

ra0001p217

Abstract unavailable

ra0001p218

Antioxidant vitamins supplementation decreases motility in both fresh and refrigerated ram semen

Parraguez Victor H Palomino Jaime Arancibia Massiel Cofre Eileen Urquieta Bessie Raggi Luis Gonzalez-Bulnes Antonio

¹Faculty of Veterinary Sciences, University of Chile, Santiago, Chile; ²Faculty of Agricultural Sciences, University of Chile, Santiago, Chile; ³International Center for Andean Studies, University of Chile, Santiago, Chile; ⁴NIA, Madrid, Spain.

ra0001p219

Crosstalk of TGF[beta] superfamily with Smad1 in testis

Chan Kai-Hui Galuska Sebastian P Loveland Kate L Lutz Konrad

¹Justus Liebig University (FB Medizin), Giessen, Germany; ²Monash University, Clayton, Victoria, Australia.

ra0001p220

Meiotic and post-meiotic germ cells up-regulate blood-testis barrier function

Olcorn Justine Samantha Rainczuk Katarzyna Eliza Foo Caroline Haverfield Jenna T Stanton Peter G

¹MIMR-PHI Institute of Medical Research, Clayton, Victoria, Australia; ²Prince Henry’s Institute, Clayton, Victoria, Australia.

ra0001p221

The RNA-binding protein Musashi-2 (MSI2) controls mRNA processing and translational regulation via interactions with SFPQ and PIWIL1 during mammalian spermatogenesis

Sutherland Jessie M Sobinoff Alexander P Redgrove Kate Davidson Tara-Lynne Siddall Nicole A Koopman Peter Hime Gary R McLaughlin Eileen

¹University of Newcastle, Callaghan, New South Wales, Australia; ²Institute for Molecular Bioscience, Brisbane, Queensland, Australia; ³University of Melbourne, Parkville, Victoria, Australia; ⁴The University of Queensland, Brisbane, Queensland, Australia.

ra0001p222

GATA4 serine 105 phosphorylation is required for testicular steroidogenesis in the mouse

Viger Robert Sab Alain Boulende Bouchard Marie France Bergeron Francis Brousseau Catherine Tremblay Jacques J Pilon Nicolas

¹Laval University, Quebec City, Quebec, Canada; ²Université du Québec à Montréal, Montreal, Quebec, Canada.

ra0001p223

Maintenance and amplification of testicular tumors by subcutaneous grafting in zebrafish

Kawasaki Toshihiro Sakai Noriyoshi

National Institute of Genetics, Mishima, Japan.

ra0001p224

Effect of aromatase on sperm penetration and embryo cleavage rate during IVF in pig

Oh Jong-Nam Hwang Jae Yeon Lee Dong-Kyung Lee Chang-Kyu

Seoul National University, Seoul, Republic of Korea.

ra0001p225

Effects of different concentration of Escherichia coli on boar sperm quality and field fertility

Sa Soojin Choi Sunho Kim Hyunjong Cho Kyuho Hong Joonki Kim Duwan Kim Younghwa Park Juncheol

National Institute of Animal Science, Cheonan, Republic of Korea.

ra0001p226

Single-cell level dynamics of mouse spermatogenic stem cells after transplantation

Nakamura Yoshiaki Yoshida Shosei

National Institute for Basic Biology, Okazaki, Japan.

ra0001p227

Effect of ascorbic acid 2-glucoside supplementation on sperm characteristics during semen cryopreservation in Korean native pig

Park Juncheol Sa Soojin Choi Sunho Kim Hyunjong Cho Kyuho Hong Joonki Kim Duwan Kim Younghwa

National Institute of Animal Science, Cheonan, Republic of Korea.

ra0001p228

Trehalose in glycerol-free freezing extender preserves mitochondria membrane potential of post-thaw boar spermatozoa

Athurupana Rukmali Funahashi H

Okayama University, Okayama, Japan.

ra0001p229

Abstract unavailable

ra0001p230

Knocking-down detrimental seminal protein PDC-109 minimizes cryoinjury to bull spermatozoa

Srivastava N Srivastava S K Ghosh S K Kumar Amit Andonissamy J Ponraj Perumal

¹Indian Veterinary Research Institute, Bareilly, India; ²Central Institute for Research on Buffaloes, Hisar, India.

ra0001p231

Ultrastructural analysis of epididymis of agouti (Dasyprocta spp) during sexual development

Arroyo Maria Angelica M Santos Paulo Ramos Silva Oliveira Moacir Franco de Assis Neto Antonio Chaves

¹School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil; ²Universidade Federal Rural do Semi-Árido, Mossoró, Brazil.

ra0001p232

Ultrastructural analysis of spermatozoa in agoutis during sexual development

Arroyo Maria Angelica M Santos Paulo Ramos Silva Oliveira Moacir Franco de Assis Neto Antonio Chaves

¹School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil; ²Universidade Federal Rural do Semi-Árido, Mossoró, Brazil.

ra0001p233

Characterisation of Gankyrin expression in testicular germ cell cancer

Moll Maria Elena Camacho Macdonald Joni Mitchell Rod Thomas

¹University of Edinburgh, Edinburgh, UK; ²MRC Centre for Reproductive Health, Edinburgh, UK.

ra0001p234

Tamoxifen inducible Cre systems: adverse effects of low dose tamoxifen treatments on the male reproductive tract

Patel Saloni H Milne Laura Sharpe Richard Smith Lee B

University of Edinburgh, Edinburgh, UK.

ra0001p235

Abstract unavailable

ra0001p236

Involvement of peroxisomes in steroid synthesis in Leydig cells and their role for male fertility

Kamalyan Lilit Vijayan Vijith Brauns Ann-Kristin Colasante Claudia Schuler Gerhard Luers Georg H Baumgart-Vogt Eveline

¹Justus-Liebig Universität, Giessen, Germany; ²UKE, Hamburg, Germany.

ra0001p237

Targeting high mobility group box protein 1 ameliorates experimental autoimmune orchitis

Aslani Ferial Schuppe Hans-Christian Guazzone Vanesa A Bhushan Sudhanshu Lustig Livia Meinhardt Andreas Fijak Monika

¹Justus Liebig University of Giessen, Giessen, Germany; ²Universidad de Buenos Aires, Buenos Aires, Argentina.

ra0001p238

Effect of addition post-thaw of homologous seminal plasma on quality of donkey frozen semen

de Luna Freire Oliveira Pedro Victor Oliveira Jose Victor Neto Carlos Ramires Sancler-Silva Yame F R Freitas Dell'aqua Camila P Papa Frederico O

¹College of Veterinary Medicine and Animal Science, (UNESP)/Univ Estadual Paulista, Botucatu, Brazil; ²UNESP–BOTUCATU, Botucatu, Brazil.

ra0001p239

Difference between of homologous and heterologous seminal plasma addition on post-thawn donkey frozen semen

de Luna Freire Oliveira Pedro Victor Oliveira Jose Victor Sancler-Silva Yame F R Neto Carlos Ramires Freitas Dell'aqua Camila P Papa Frederico O

¹College of Veterinary Medicine and Animal Science, (UNESP)/Univ Estadual Paulista, Botucatu, Brazil; ²UNESP–BOTUCATU, Botucatu, Brazil.

ra0001p240

Evaluation of donkey frozen semen quality in different freezing conditions

de Luna Freire Oliveira Pedro Victor Oliveira Jose Victor Sancler-Silva Yame F R Neto Carlos Ramires Oliveira Marcelo L F Papa Frederico O

¹College of Veterinary Medicine and Animal Science, (UNESP)/Univ Estadual Paulista, Botucatu, Brazil; ²UNESP–BOTUCATU, Botucatu, Brazil; ³University of São Paulo (USP), Pirassununga, Brazil.

ra0001p241

Acute reduction of Sertoli cell numbers during development leads to a subsequent reduction in sperm numbers in adulthood

Darbey Annalucia L O'Shaughnessy Peter Pitetti Jean-Luc Nef Serge Smith Lee Rebourcet Diane

¹University of Edinburgh, Edinburgh, UK; ²University of Glasgow, Glasgow, UK; ³Univeristy of Geneva Medical School, Geneva, Switzerland.

ra0001p242

Differential expression of proteins from the human sperm head and its relation to infertility

Baruah Binita Basnet Kola Srujana Rukmangadachar Lokesh Chaturvedi Pradeep Srinivasan A

¹All India Institute of Medical Sciences, Guwahati, India; ²AIIMS, New Delhi, India.

ra0001p243

Abstract unavailable

ra0001p244

Study of spermatogonia stem cells from domestic animals: derivation and in vitro culture

Song Hyuk Lee Won Young Lee Kyung Hoon

Konkuk University, Chungju, Republic of Korea.

ra0001p245

Proteomic approach of cryo-damage in bovine spermatozoa

Yoon Sung-Jae Kwon Woo-Sung Rahman Saidur Lee June-Sub Park Yoo-Jin You Young-Ah Pang Myung-Geol

¹Chung-Ang University, Anseong, Republic of Korea; ²Chung-Ang University, Gyungki-Do, Republic of Korea.

ra0001p246

Effect of Sertoli cell ablation on testicular function in adult mouse

Rebourcet Diane O'Shaughnessy Peter Monteiro Ana Mitchell Rod Milne Laura Smith Lee

¹University of Edinburgh, Edinburgh, UK; ²University of Glasgow, Glasgow, UK; ³MRC Centre for Reproductive Health, Edinburgh, UK.

ra0001p247

Comparision of DNA fragmentantion and plasma membrane integrity between chilled and frozen semen of bulls

de Mello Papa Patricia Neto Carlos Ramires Guasti Priscilla Nascimento Maziero Rosiara Rosaria Dias Sancler-Silva Yame F R Monteiro Gabriel A Dell'aqua Camila P Freitas Junqueira Cassio Renesto Martin Ian Alvarenga Marco Antonio Papa Frederico O Aqua Junior Jose Antonio Dell'

¹Paulo State University, Botucatu, Brazil; ²College of Veterinary Medicine and Animal Science, (UNESP)/Univ Estadual Paulista, Botucatu, Brazil; ³UNESP–BOTUCATU, Botucatu, Brazil.

ra0001p248

Acceptability of New Zealand imported semen to the Japanese dairy farmers

Ozawa Takeyuki Tamagawa Satomi

¹Nippon Veterinary and Life Science University, Musashino-shi, Japan; ²Graduate School of Nippon Veterinary and Life Science University, Musashino-shi, Japan.

ra0001p249

Exome sequencing identified QRICH2 as a candidate gene responsible for stump-tailed sperm defects in Chinese consanguineous male offspring

Yue Huanxun Sun Dan Sun Huaqin Xu Wenming

West China Second University Hospital, Chengdu, China.

ra0001p250

Manipulating the germ stem cell 'niche' to elucidate the origins of testicular germ cell cancer

Macdonald Joni Mitchell Rod

¹University of Edinburgh, Edinburgh, UK; ²MRC Centre for Reproductive Health, Edinburgh, UK.

ra0001p251

Roles of testicular macrophage in Leydig cell proliferation in colony-stimulating factor treated mice

Tsai Tina Sauter Kristin Milne Laura Hume David Smith Lee

¹Queens Medical Research Institute, MRC Centre for Reproductive Health, Edinburgh, UK; ²The Roslin Institute, Midlothian, UK; ³University of Edinburgh, Edinburgh, UK.

ra0001p252

Panel of MABs to sperm surface proteins as tool for monitoring of sperm-zona pellucida receptors localization and identification

Zigo Michal Dorosh Andriy Pohlova Alzbeta Jonakova Vera Sulc Miroslav Postlerova Pavla

¹Laboratory of Reproductive Biology, Institute of Biotechnology, The Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20, Prague 4, Czech Republic; ²Department of Biochemistry, Faculty of Science, Charles University, Hlavova 8, 128 43, Prague 2, Czech Republic; ³Institute of Microbiology, The Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20, Prague 4, Czech Republic.

ra0001p253

Buffalo (Bubalus bubalis) spermatozoa based biosensor for the detection of Cereulide toxin from milk powder

Bamnia Meenakshi Kaul Gautam

Animal Biochemistry Division, National Dairy Research Institute, Karnal, India.

ra0001p254

Canine spermatogonial stem cells transfected with GFP reporter gene transplanted in recipient teste mice

Pieri Naira Caroline Godoy de Souza Aline Fernanda Mancanares Ana Carolina Furlanetto Casals Juliana Barbosa Roballo Kelly Cristine Bressan Fabiana Oliveira Lilian Jesus Ambrosio Carlos Eduardo Martins Daniele Santos

¹University of Sao Paulo, Pirassununga, Brazil; ²School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil.

ra0001p255

Mass-specific metabolic rate influences sperm performance through energy production in mammals

Tourmente Maximiliano Roldan Eduardo R S

Museo Nacional de Ciencias Naturales (CSIC), Madrid, Spain.

ra0001p256

The role of estrogen and the effect of age on porcine testicular extracellular matrix

Irving-Rodgers Helen James Chantel Hummitzsch Katja Schwartz Jeff Rodgers Ray Conley Alan Berger Trish

¹Griffith University, Southport, Queensland, Australia; ²The University of Adelaide, Adelaide, South Australia, Australia; ³University of California, Davis, California, USA.

ra0001p257

Testicular chemical pollutants alter sperm vitality, motility, and morphology: comparative studies in the human and dog

Byers Andrew Sumner Rebecca Richardson Holly-May Stratford Claire Cammack Thomas Moxon Rachel Tomlinson Mathew Naeem Asad Craigon Jim England Gary C W Lea Richard

¹University of Nottingham, Loughborough, UK; ²Guide Dogs for the Blind Association, Bishop’s Tachbrook, Leamington Spa, UK; ³Fertility Unit, Nottingham, UK; ⁴AIR University, Islamabad, Pakistan; ⁵The University of Nottingham, Sutton Bonington, UK.

ra0001p258

SSP411 a sperm factor involved in oocyte cleavage

Liu Miao Gu Yihua Wang Jian Lu Yi Yuan Yao Wu Bin Chen Ching-Ling C Shi Huijuan

¹Fudan University, Shanghai, China; ²The Shanghai Institute of Planned Parenthood Research (SIPPR), Shanghai, China; ³Population Council, NewYork, New York, USA.

ra0001p259

In vitro effects of hydrogen peroxide on ALF expression in male mouse germ cells

Habas Khaled S A Anderson Diana Brinkworth Martin H

University of Bradford, Bradford, UK.

ra0001p260

Time-lapse imaging as a tool to evaluate contractile cell function and sperm transport

Mietens Andrea Tasch Sabine Eichner Gerrit Feuerstacke Caroline Middendorff Ralf

¹Justus Liebig University, Giessen, Germany; ²Institute of Anatomy and Cell Biology, Giessen, Germany.

ra0001p261

Abstract unavailable

ra0001p262

Comparison between egg yolk-based and coconut water-based extenders to freeze buffalo semen

Zorzetto Mariana Sancler-Silva Yame Dell'aqua Camila Freitas Neto Carlos Ramires Zocca Saulo Ramos Alcides Oba Eunice Derussi Ana Augusta Pagnano

Unesp Botucatu, Botucatu, Brazil

ra0001p263

Placental phenotype of Turner mouse model: differences between XmO and XpO

Lim Shujing Jane Ojarikre Andrew Chuva de Sousa Lopes Susana M

¹University of Edinburgh, Edinburgh, UK; ²MRC National Institute for Medical Research, London, UK; ³Leiden University Medical Center, Leiden, The Netherlands.

ra0001p264

The tight junction molecules expression and localization in mouse placenta

Ahn Changhwan Jeung Eui-Bae

Chungbuk National University, Cheongju, Republic of Korea.

ra0001p265

The acyl-CoA dehydrogenase, very long chain (ACADVL) expression under hypoxic condition in human placental cell line (BeWo)

Shin Eun-Kyeong Lee Dongoh Jeung Eui-Bae

Chungbuk National University, Cheongju, Republic of Korea.

ra0001p266

The regulation of calcium transport channels, TRPV5, TRPV6, PMCA1, NCKX3, and CaBP-9k in ro410960-treated mice of pregnancy

Yang Hyun Choi Young-Kwon Jeung Eui-Bae

Chungbuk National University, Cheongju, Republic of Korea.

ra0001p267

Expression and regulation of N-myc downregulated gene 2 in mouse uterus during the peri-implantation period

Zhang Xuan He Yaping Sun Zhaogui Wang Jian

The Shanghai Institute of Planned Parenthood Research (SIPPR), Shanghai, China.

ra0001p268

The influence of aldrin and dieldrin on the function of bovine uterus and ovary in vitro

Wrobel Michal H Grzeszczyk Marlena Mlynarczuk Jaroslaw Kotwica Jan

Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland.

ra0001p269

PGRMC1 protein expression in the canine endometrium

Bartel Cordula Walter Ingrid

¹Department for Pathobiology, Histology and Embryology, Vetmeduni Vienna, Institute of Anatomy, Vienna, Austria; ²Facility for Research, University of Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria.

ra0001p270

Do maternal obesity and mood disorders correlate with changes in placental glucocorticoid transporter gene expression?

Mina Theresia H Davidson Callam Taylor Ashley Norman Jane E Reynolds Rebecca M

University of Edinburgh, Edinburgh, UK.

ra0001p271

Estradiol promotes differentiation of uterine natural killer cells during establishment of pregnancy

Gibson Douglas A Critchley Hilary O D Saunders Philippa T K

University of Edinburgh, Edinburgh, UK.

ra0001p273

A role for androgens in the regulation of endometrial breakdown and repair in a mouse model of menstruation

Cousins Fiona L Murray Alison A Critchley Hilary O D Saunders Philippa T K

University of Edinburgh, Edinburgh, UK.

ra0001p274

Apoptosis and neutrophil infiltration in the human endometrium at menstruation, recapitulated in mouse model of induced menstruation

Armstrong Gregory M Murray Alison Maybin Jacqueline A Rossi Adriano G Critchley Hilary O D

¹Queen’s Medical Research Institute, Centre for Reproductive Health, University of Edinburgh, Edinburgh, UK; ²Queen’s Medical Research Institute, Centre for Inflammation Research, University of Edinburgh, Edinburgh, UK.

ra0001p275

Glucocorticoids trigger macrophage migration inhibitory factor (MIF) secretion by decidualized human endometrial stromal cells in vitro: the modulatory effect of Bisphenol A

Mannelli Chiara Szostek Anna Ciaffarafa Sara Carotenuto Claudiopietro Ietta Francesca Romagnoli Roberta Piotrowska-Tomala Katarzyna Paulesu Luana Skarzynski Dariusz

¹Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland; ²University of Siena, Siena, Italy.

ra0001p276

Abstract unavailable

ra0001p277

Bovine placental lactogen is cleaved by matrix metalloproteinases and resulted 25k N-terminal fragments inhibit the proliferation of vascular endothelial cells

Sasaki Shobu Hayashi Ken-Go Hosoe Misa Kizaki Keiichiro Hashizume Kazuyoshi Takahashi Toru

¹The University of Tokyo, Kashiwa, Japan; ²National Institute of Agrobiological Sciences, Tsukuba, Japan; ³Iwate University, Morioka, Japan.

ra0001p278

An estradiol metabolite 2-methoxyestradiol exhibits estrogen-like effect in vitro and in vivo

Lee Ji-Sun Jeung Eui-Bae

Chungbuk National University, Cheongju, Republic of Korea.

ra0001p279

Prominin-1 and -2 in uterine epithelial cells during early pregnancy

Dowland Samson Green Charmaine J Lindsay Laura A Day Margot L Murphy Chris

¹University of Sydney, Sydney, New South Wales, Australia; ²University of Sydney and Bosch Institute, Sydney, New South Wales, Australia.

ra0001p280

Trophoblast-decidual crosstalk optimises trophoblast invasion

Menkhorst Ellen Gamage Thilini Van Sinderen Michelle Cuman Carly Rainczuk Adam Stephens Andrew N Dimitriadis Eva

¹MIMR-PHI Institute of Medicial Research, Melbourne, Victoria, Australia; ²MIMIR-PHI Institute, Clayton, Victoria, Australia.

ra0001p281

Caveolins redistribute in uterine epithelial cells during early pregnancy: role in epithelial polarisation

Madawala Romanthi J Dowland Samson Poon Connie E Murphy Christopher

The University of Sydney, Sydney, New South Wales, Australia.

ra0001p282

Interleukin-11 is critical for placentation in mice

Winship Amy Menkhorst Ellen Van Sinderen Michelle Dimitriadis Eva

MIMR-PHI Institute of Medical Research, Melbourne, Victoria, Australia.

ra0001p283

Abstract unavailable

ra0001p284

Distinct sex-specific gene expression changes in the human placenta in association with childhood allergy at 2 years

Tuck Astrud Osei-Kumah Annette Saif Zarqa Clifton Vicki L

University of Adelaide, Elizabeth Vale, South Australia, Australia.

ra0001p285

The EpCAM-claudin 7 protein complex decreases during early pregnancy in the rat

Poon Connie E Day Margot L Murphy Christopher

The University of Sydney, Sydney, Australia.

ra0001p286

Short-term administration of ulipristal acetate modulates endometrial sex steroid receptor expression and cell proliferation markers

Murray Alison A Shaw Grace G Nicol Moira Williams Alistair R W Saunders Philippa T K Critchley Hilary O D

University of Edinburgh, Edinburgh, UK.

ra0001p287

Periconceptional alcohol exposure programs sex specific hyperinsulinemia possibly through dysregulation of placental O-linked glycosylation

Gardebjer Emelie M McMahon Kathryn Moritz Karen M Pantaleon Marie

The University of Queensland, St Lucia, Queensland, Australia.

ra0001p288

Analysis of EMG signal linear synchronization in porcine uterus

Domino Malgorzata Gajewski Zdzislaw Pawlinski Bartosz Zabielski Romuald Aniolek Olga

Warsaw University of Life Scienses, Warsaw, Poland.

ra0001p289

Sex specific effects of maternal dietary protein upon uterine blood flow and fetal growth

Hernandez-Medrano Juan H Copping Katrina J Hoare A S Wapenaar Wendela Grivell Rosalie Callaghan Matthew McMillen Caroline Rodgers Ray Perry Viv E A

¹University of Nottingham, Loughborough, UK; ²University of Adelaide, Adelaide, South Australia, Australia; ³South East Vets, Adelaide, South Australia, Australia; ⁴Ridley Agriproducts, Brisbane, Queensland, Australia; ⁵University of Newcastle, Newcastle, New South Wales, Australia.

ra0001p290

Effect of pentraxin 3 on inflammation-induced fetal loss

Bonfiglio Rita Camaioni Antonella Palone Francesca Bottazzi Barbara Sironi Marina Garlanda Cecilia Salvatori Giovanni Mantovani Alberto Salustri Antonietta

¹University of Rome Tor Vergata, Rome, Italy; ²Humanitas Clinical and Research Center, Milan, Italy; ³Sigma-tau Industrie Farmaceutiche Riunite S.p.A, Rome, Italy.

ra0001p291

Glycoforms of [alpha]-2-macroglobulin in uterine fluid, are altered during the menstrual cycle, and differ between fertile and infertile women

Edgell Tracey A. Fitzgerald Harriet Salamonsen Lois

MIMR-PHI, Clayton, Victoria, Australia.

ra0001p292

Non-cytopathic BVDV suppressed innate immune response in bovine endometrial cells exposed to bacterial lipopolysaccharide

Oguejiofor Chike Fidelis Cheng Zhangrui Anstaett Olivia Brownlie Joe Fouladi-Nashta Ali A. Wathes D Claire

Royal Veterinary College, Hatfield, Hertfordshire, UK.

ra0001p293

Remodelling-associated processes during postpartum uterine involution in mice

Menzies Fiona M Burton Laura Ahmed Humera Oldham Rachel S. Higgins Claire A Nelson Scott M Nibbs Robert J B

¹University of the West of Scotland, Paisley, UK; ²University of Glasgow, Glasgow, UK.

ra0001p294

Investigating the role of androgens in endometriosis

Simitsidellis Ioannis Greaves Erin Collins Frances Horne Andrew W Saunders Philippa T K

University of Edinburgh, Edinburgh, UK.

ra0001p295

Evaluation of protein profile in uterine secretion on early cyclic and gestational diestrus in bitches

Derussi Ana Augusta Pagnano Volpato Rodrigo de Freitas Guaitolini Carlos Renato Ackermann Camila Louise Taffarel Marilda Onghero Cardoso Guilherme Schiess Villaverde Ana Izabel Silva Balbin de Souza Fabiana Ferreira Lopes Maria Denise

¹School of Veterinary Medicine and Animal Science- UNESP- Botucatu, Botucatu, Brazil; ²São Paulo State University, Botucatu, Brazil; ³State University of Maringa, Maringa, Brazil; ⁴State University of Londrina, Londrina, Brazil.

ra0001p296

Does decidual macrophage polarisation affect trophoblast behaviour in co-culture studies?

Buckley Rebecca Dumitriu Ingrid Whitley Guy S Cartwright Judith

¹Division of Biomedical Sciences, St George’s University of London, London, UK; ²Division of Clinical Sciences, St George’s University of London, London, UK.

ra0001p297

Decidual stromal cell regulation of trophoblast in first trimester pregnancies with normal and impaired spiral artery remodelling

James-Allan Laura Wallace Alison E Whitley Guy S Cartwright Judith

St George’s University of London, London, UK.

ra0001p298

Effect of maternal diet on the amino acid composition of human uterine fluid

Kermack Alexandra Jayne Cheong Ying Brook Nick Macklon Nick Houghton Franchesca D

¹University of Southampton, Southampton, UK; ²Complete Fertility Centre, Southampton, UK.

ra0001p299

Reproductive performance, oxidative enzyme and gonadotropins status of rabbit does fed organic selenium as dietary supplement

Omeje Valentine Machebe Ndubuisi Samuel Ezekwe Arinze

¹University of Nigeria, Nsukka, Nigeria; ²Kinki University, Nara, Japan.

ra0001p300

Secreted phosphoprotein 1 (SPP1), vascular endothelial growth factor A (VEGFA), heparinase (HPSE), hypoxia-inducible factor (HIF1A) and uteroferrin (ACP5) gene expression in uterine and placental tissues from Large White-Landrace (LW-LD), hyperprolific Large White (hLW) and Meishan (MS) pigs

Hernandez Silvia C Hogg Charis O Ait-Ali Tahar Ashworth Cheryl Joy

University of Edinburgh, Edinburgh, UK.

ra0001p301

Abstract unavailable

ra0001p302

Effect of progesterone supplementation on endometrial receptivity for implantation in the pig

Blitek Agnieszka Szymanska Magdalena

Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland.

ra0001p303

The relaxing effect of dihydrotestosterone on myometrial smooth muscle contraction is mediated via blockage of intracellular calcium increase and inhibition of myosin light chain phosphorylation

Makieva Sofia Saunders Philippa T K Norman Jane E

University of Edinburgh, Edinburgh, UK.

ra0001p304

Detection of circulating miRNAs in the maternal serum during early pregnancy in the pig by qRT-PCR and digital RT-PCR

P Reliszko Zaneta Gajewski Zdzislaw M Kaczmarek Monika

¹Laboratory of Molecular Biology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn; ²Department for Large Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Nowoursynowska 100, 02-797 Warsaw, Poland.

ra0001p305

Inhibition of the polyamine pathway induces an embryonic diapause-like state in the mouse embryo

Catherine Fenelon Jane Banerjee Arnab Murphy Bruce D

Université de Montréal, Saint-Hyacinthe, Canada.

ra0001p306

Effect of flax seed (Linum usitatissimum) extract on modulation of prostaglandin E2 production by buffalo endometrial stromal cells cultured in vitro

Kumar Singh Sanjay Sharma Chethan G Nongsiej Jessiehun Singh R P Kumar Agarwal Sudhir

¹School of Biosciences, University of Nottingham, Leicestershire, UK; ²IVRI, Bareilly, India; ³CIRG, Mathura, India.

ra0001p307

Detection of SPAM1 in the bovine oviductal fluid

Salvador Acuna Omar Jimenez-Movilla Maria Vilella Ismael Jara Lourdes Canovas Sebastian Coy Pilar Aviles Manuel Izquierdo Rico Maria Jose

¹University of Murcia, Murcia, Spain; ²LARCEL, Sevilla, Spain.

ra0001p308

Comparison between the effect of hCG and GnRH analogue (buserelin) treatment post laparoscopic insemination on reproductive performance of ewes

Swelum Ayman Abdel-Aziz Al-Owaimer Abdullah N Abouheif Mohamed A

College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia.

ra0001p309

Novel sequence variations in exon 25 of JHDM2A among overweight and obese sub/infertile males in South India

Najafi Mohsen Malini Suttur S

University of Mysore, Mysore, India.

ra0001p310

Incerement of proinflammatory cytokine (IL6) and lipid peroxidation in obese infertile males may lead to more impaired seminogram compared to non-obese infertile males

Najafi Mohsen Aarabi Mahmoud Yousefi Azin Nasri Soroush Ajami Sanaz Malini Suttur S

¹University of Mysore, Mysore, India; ²School of Medicine, Queen’s University, Kingston, Canada; ³Islamic Azad University, Sari Branch, Sari, Iran; ⁴Milad Medical Genetics Laboratory, Sari, Iran.

ra0001p311

Metallothionein gene expression in green mussels (Perna viridis) as a bioindicator for heavy metal on the North Coast of Java and its impact on the development of reproductive organs

Irnidayanti Yulia Septiana Shofy Layli Fitri Apriliana

Jakarta State of Universiy, Jakartra, Indonesia.

ra0001p312

Allopregnanolone promotes angiogenesis and inhibits apoptosis in the corpora lutea in rat ovarian cycle

Laconi Myriam Raquel Cabrera Ricardo Jorge Parborell Fernanda

¹IMBECU - CONICET, Mendoza, Argentina; ²IByME - CONICET, Buenos Aires, Argentina.

ra0001p313

Abstract unavailable

ra0001p314

Protein hormone proteolysis in target cell endosomes and lysosomes and release of previously unrecognized signaling information

Campbell Kenneth L Haspel Nurit Dominas Barbara Mason Briana Calixte Wagner Bigos Ronald Taguzem G Florent Vader Umaben Palmstrom Noelle Steinbruck Jeremy Solchenberger Sherry Diallo Fatoumata

University of Massachusetts Boston, Boston, Massachusetts, USA.

ra0001p315

Deficiency of monoclonal nonspecific suppressor factor beta (MNSF[beta]) gene leaded to the early loss of pregancy in mice

He Yaping Zhang Xuan Wang Jian Sun Zhaogui

The Shanghai Institute of Planned Parenthood Research (SIPPR), Shanghai, China.

ra0001p316

Analysis of marsupial ZP: molecular and phylogenetic approach

Nicolas C Moros Rico M J Izquierdo Holt W V Chevret P Lopez-Bejar M Martinez-Nevado E Ballesta J Aviles M

¹University of Murcia, Murcia, Spain; ²Institute of Zoology, London, UK; ³Université Claude Bernard Lyon 1, Lyon, France; ⁴Universitat Autònoma de Barcelona, Barcelona, Spain; ⁵Zoo-Aquarium Madrid, Madrid, Spain.

ra0001p317

Transmission of lethal phenotype in a Mendelian fashion by genetically modified pigs that underwent blastocyst complementation

Matsunari Hitomi Watanabe Masahito Umeyama Kazuhiro Nakano Kazuaki Nagaya Masaki Nakauchi Hiromitsu Nagashima Hiroshi

¹Meiji University International Institute for Bio-Resource Research (MUIIBR), Kawasaki, Japan; ²Laboratory of Developmental Engineering, Department of Life Sciences, Meiji University, Kawasaki, Japan; ³Institute of Medical Science, The University of Tokyo, Tokyo, Japan.

ra0001p318

Reproductive and toxic effects of methanol extract of Alchornea cordifolia leaves

Ajibade Temitayo Olabisi

University of Ibadan, Ibadan, Nigeria.

ra0001p319

Pinacidil, a KATP channel opening drug, protects human oocytes against metabolic stress

Fernandes Goncalo M T Gall Mandy Drew Ellen Barratt Evelyn Madamidola Oladipo Brown Sean G Milne Alison M da Silva Sarah Martins Barratt Christopher Jovanovic Aleksandar

¹University of Dundee, Dundee, UK; ²Ninewells Hospital, Dundee, UK; ³Abertay University, Dundee, UK.

ra0001p320

Fetal overexposure to androgens, not estrogens, leaves a permanent legacy in terms of adrenal function in offspring

Ramaswamy Seshadri Grace Cathal Howie Forbes Connolly Fiona Konovalova Julia McNeilly Alan S Duncan W Colin Rae Mick

¹Edinburgh Napier University, Edinburgh, UK; ²University of Edinburgh, Edinburgh, UK.

ra0001p321

Inhibition of the TLR4 signalling pathway protects from intrauterine heat-killed Escherichia coli-induced pre-term delivery

Chin Peck Yin Dorian Camilla Hutchinson Mark Robertson Sarah

The University of Adelaide, Adelaide, Australia.

ra0001p322

Effect of doses and time post-injection on hormonal induction of spermiation in Bufo calamita (Anura: Bufonidae)

Lopez Elia Alonso Kouba Andrew J Arregui Lucia

¹Universidade de Santiago de Compostela, Santiago de Compostela, Spain; ²Memphis Zoo, Memphis, Tennessee, USA; ³Universidad Autónoma de Madrid, Madrid, Spain.

ra0001p323

Effects of early exposure to bisphenol A, indole-3-carbinol, and/or genistein and the prostatic development in male rats offspring

Brandt Joyce Zalotti Pinho Cristiane Figueiredo Silveira Livia Teresa Ribeiro Georgete Paulo Cesar Bozano Jose Eduardo Barbisan Luis Fernando Scarano Wellerson Rodrigo

¹Institute of Biosciences, Sao Paulo State University, UNESP, Botucatu, Brazil; ²School of Medicine, UNESP, Botucatu, Brazil.

ra0001p324

Chemical fingerprinting of embryotoxic mineral oil

Morbeck Dean Gallacher Christopher Kalin Robert M

¹Mayo Clinic, Rochester, New York, USA; ²University of Strathclyde, Glasgow, UK.

ra0001p325

Abstract unavailable

ra0001p326

Establishment of immortalized neuronal cell lines derived from fetal goat hypothalamus

Suetomi Yuta Tsukamura Hiroko Ohkura Satoshi Matsuda Fuko

Nagoya University, Nagoya, Japan.

ra0001p327

Development of novel neurokinin-3 receptor agonists that potently stimulate the GnRH pulse generator

Yamamura Takashi Misu Ryosuke Wakabayashi Yoshihiro Ohkura Satoshi Oishi Shinya Fujii Nobutaka Okamura Hiroaki

¹National Institute of Agrobiological Sciences, Tsukuba, Japan; ²Kyoto University, Kyoto, Japan; ³Nagoya University, Nagoya, Japan.

ra0001p328

Changes in gene expressions induced by perinatal estrogen related to the brain sexual differentiation in rodents

Watanabe Youki Sakakibara Mototsugu Uenoyama Yoshihisa Minabe Shiori Deura Chikaya Nakamura Sho Maeda Kei-ichiro Tsukamura Hiroko

¹Nagoya University, Nagoya, Japan; ²The University of Tokyo, Bunkyo-ku, Japan.

ra0001p329

Metabolic reprogramming may underpin the aetiology of endometriosis

Young Vicky J Brown Jeremy K Maybin Jacqueline A Duncan W Colin Horne Andrew W

¹University of Edinburgh, Edinburgh, UK; ²Queen’s Medical Research Institute, Edinburgh, UK.

ra0001p330

Deletion of the C-terminal region of OVGP1 affects porcine IVF

Algarra Blanca Han Ling Aviles Manuel Coy Pilar Jovine Luca Jimenez-Movilla Maria

¹University of Murcia, Murcia, Spain; ²Karolinska Institutet, Huddinge, Sweden.

ra0001p331

Androgen receptor-signalling in the male pituitary is dispensable for normal LH secretion but suppresses prolactin production

Ferreira Maria Tedim Curley Michael Milne Laura Smith Lee O'Hara Laura

¹University of Edinburgh, Edinburgh, UK; ²MRC Centre for Reproductive Health, Edinburgh, UK.

ra0001p332

Selective expression of two DAZL isoforms in human fetal ovary but not testis

Rosario Roseanne Anderson Richard

University of Edinburgh, Edinburgh, UK.

ra0001p333

Development of knock-in somatic cells to produce human FGF2 protein on the bovine [beta]-casein gene locus using F2A self-processing peptide

Kim Se Eun Jeong Young-Hee Kim Yeong Ji Kang Man-Jong

College of Agriculture and Life Science, Chonnam National University, Gwangju, Republic of Korea.

ra0001p334

Neurokinin B activates synchronized intracellular Ca2+ oscillations in KNDy neurons obtained from the hypothalamic arcuate nucleus of Kiss1-GFP transgenic mice

Ikegami Kana Minabe Shiori Ieda Nahoko Goto Teppei Abe Hitomi Sanbo Makoto Hirabayashi Masumi Maeda Kei-ichiro Tsukamura Hiroko Uenoyama Yoshihisa

¹Nagoya University, Nagoya, Japan; ²National Institute for Physiological Science, Okazaki, Japan; ³The University of Tokyo, Bunkyo-ku, Japan.

ra0001p335

Autophagy supports the survival of the conceptus obtained by assisted reproductive technologies

Toschi Paola Czernik Marta Zacchini Federica Loi Pasqualino Ptak Grazyna Ewa

University of Teramo, Teramo, Italy.

ra0001p336

Abstract unavailable

ra0001p337

Gonadotropin-independent follicle development in the Kiss1/- female rats

Shirota Mariko Kawashima Jun Shirota Kinji Uenoyama Yoshihisa Nakamura Sho Ikegami Kana Maeda Kei-ichiro Tsukamura Hiroko

¹Azabu University, Sagamihara, Japan; ²Nagoya University, Nagoya, Japan; ³The University of Tokyo, Bunkyo-ku, Japan.

ra0001p338

Hypothalamic arcuate nuleus-specific enhancer for kisspeptin expression of female mice

Goto Teppei Tsukamura Hiroko Tomikawa Junko Abe Hitomi Fukanuma Tatsuya Imaura Takuya Takase Kenji Sanbo Makoto Tomita Koichi Hirabayashi Masumi Maeda Kei-ichiro Uenoyama Yoshihisa

¹Nagoya University, Nagoya, Japan; ²Kyoto University, Kyoto, Japan; ³National Institute for Physiological Sciences, Okazaki, Japan; ⁴The University of Tokyo, Bunkyo-ku, Japan.

ra0001p339

Proteome-wide changes in liver function by continuous exposure to sewage sludge

Filis Panagiotis Turner Emily Eaton O'Shaughnessy Peter Fowler Paul A

¹University of Aberdeen, Aberdeen, UK; ²University of Glasgow, Glasgow, UK.

ra0001p340

Determination of expression of MFGE8 in porcine oviduct and uterus

Guillen-Martinez Ascension Nicolas Carla Moros Acuna Omar Salvador Rico Maria Jose Izquierdo Aviles Manuel

¹Facultad de Medicina, Universidad de Murcia, Murcia, Spain; ²University of Murcia, Espinardo, Spain; ³University of Murcia, Murcia, Spain.

ra0001p341

Mechanisms underlying pituitary microvascular remodelling in thoroughbred horses during the annual reproductive cycle

Yeomans Angus Thompson Nichol Castle-Miller Jennifer Bates David O Tortonese Domingo

¹University of Bristol, Bristol, UK; ²University of Nottingham, Nottingham, UK.

ra0001p342

Abstract unavailable

ra0001p343

Lack of gonadotropin release in Kiss1 knockout male rats

Uenoyama Yoshihisa Nakamura Sho Hayakawa Yuki Ikegami Kana Deura Chikaya Minabe Shiori Tomikawa Junko Goto Teppei Ieda Nahoko Sanbo Makoto Tamura Chihiro Hirabayashi Masumi Maeda Kei-ichiro Tsukamura Hiroko

¹Nagoya University, Nagoya, Japan; ²The University of Tokyo, Bunkyo-ku, Japan; ³National Institute for Physiological Science, Okazaki, Japan.

ra0001p344

The gender-specific hormone INSL3 demonstrates inter-fetal transport of hormones between male and female fetuses

Anand-Ivell Ravinder Vernunft Andreas Poeppel Caterina Ivell Richard

¹University of Nottingham, Sutton Bonington, UK; ²Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany.

ra0001p345

Does xenotoxicant-disrupted fetal sheep thyroid development persist into adulthood?

Filis Panagiotis Ramona Lauma Murray Graeme Hombach-Klonisch Sabine Klonisch Thomas O'Shaughnessy Peter Fowler Paul A

¹University of Aberdeen, Aberdeen, UK; ²Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada; ³University of Glasgow, Glasgow, UK.

ra0001p346

The role of kisspeptin for defeminization and masculinization of sexual behaviors in rats

Nakamura Sho Uenoyama Yoshihisa Ikegami Kana Tamura Chihiro Sanbo Makoto Hirabayashi Masumi Tsukamura Hiroko Maeda Kei-ichiro

¹The University of Tokyo, Bunkyo-ku, Japan; ²Nagoya University, Nagoya, Japan; ³National Institute for Physiological Science, Okazaki, Japan.

ra0001p347

Mating decreases the sulfated HNK1 carbohydrate motif over a 130 kDa glycoprotein in the rat endosalpynx

Zuniga Lidia M Nunez Teresa A Andrade Juan C Croxatto Horacio B Orihuela Pedro A Morales Patricio

¹Universidad de Antofagasta, Antofagasta, Chile; ²Universidad Andrés Bello, Santiago, Chile; ³Universidad de Santiago de Chile, Santiago, Chile.

ra0001p348

Abstract unavailable

ra0001p349

Effects of cooling and cryopreservation on sperm mitochondrial membrane and sperm motility

Sicherle Carmen Cecilia de Paula Freitas-Dell'Aqua Camila Ackermann Camila Louise Derussi Ana Augusta Pagnano Mothe Gabriele de Souza Fabiana Ferreira Papa Frederico O Lopes Maria Denise

São Paulo State University, UNESP, Botucatu, Brazil.

ra0001p350

Defining the role of sry-related HMG box 4 in male differentiation in vivo

Hartwig Sunny

Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Prince Edward Island, Canada.

ra0001p351

Granulosa cells from human small antral follicles changes gene expression during culture

Jeppesen Janni V Petersen Tonny Studsgaard Borgbo Tanni Andersen Claus Yding

¹University Hospital of Copenhagen, Copenhagen Ø, Denmark; ²Rigshospitalet, Copenhagen, Denmark.

ra0001p352

Effects of common FSH receptor single-nucleotide polymorphisms on the follicular fluid hormone profile and the granulosa cell gene expression in human small antral follicles

Borgbo Tanni Kluckova Hana MacEk Milan Chrudimska Jana Jeppesen Janni V Lindgren Ida Giwercman Yvonne Lundberg Hansen Lise Lotte Andersen Claus Yding

¹Rigshospitalet, Copenhagen, Denmark; ²University Hospital Motol, 2nd Faculty of Medicine, Charles University, Prague, Czech Republic; ³University Hospital of Copenhagen, Copenhagen Ø, Denmark; ⁴Lund University, Malmø, Sweden; ⁵Aarhus University, Aarhus C, Denmark.

ra0001p353

Telomerase reverse transcriptase is an epithelial progenitor marker in the mouse endometrium

Deane James A Breault David T Gargett Caroline E

¹MIMR–PHI Institute of Medical Research, Clayton, Victoria, Australia; ²Boston Children’s Hospital, Boston, Massachusetts, USA.

ra0001p354

Cdh1- and Cdc20-independent APC activity in mouse oocytes

Nabti Ibtissem Carroll John

¹University College London, London, UK; ²Monash University, Clayton, Victoria, Australia.

ra0001p355

In vitro differentiation of spermatogonial stem cells to functional sperm in zebrafish

Sakai Noriyoshi

National Institute of Genetics, Mishima, Japan.

ra0001p356

Effects of maternal peri-conception and first trimester protein supplementation on placental development

Edwards Jennifer L Copping Katrina J Alibhai Aziza Graham Holly Barnett Stephanie Robinson Robert S Perry Viv E A Mongan Nigel P Rutland Catrin Sian

¹University of Nottingham, Leicestershire, UK; ²University of Adelaide, Adelaide, South Australia, Australia.

ra0001p357

High testicular size is related with low intramale variation of sperm design in red deer

Ros-Santaella Jose Luis Pintus Eliana Garde Jose Julian

¹SaBio, Instituto de Investigación en Recursos Cinegéticos (IREC), CSIC-UCLM-JCCM, Ciudad Real, Spain; ²Czech University of Life Sciences, Prague, Czech Republic.

ra0001p358

Improvement of cloned blastocyst quality upon CHIR treatment of donor cells

Czernik Marta Oikawa Mami Iuso Domenico Ogura Atsuo Loi Pasqualino

¹University of Teramo, Teramo, Italy; ²RIKEN BRC, University of Tsukuba, Tsukuba, Japan.

ra0001p359

The capacity to reprogramme is an intrinsic property of adult somatic cells

Iuso Domenico Toschi Paola Anzalone Debora Agata Czernik Marta Ptak Grazyna Ewa Loi Pasqualino

University of Teramo, Teramo, Italy.

ra0001p360

The role of cell adhesion molecules in primordial follicle formation

Pepling Melissa Burton Joshua Senderoff Dana

Syracuse University, Syracuse, New York, USA.

ra0001p361

Nuclear distribution of genotoxic damage in rainbow trout (Oncorhynchus mykiss) sperm after cryopreservation

Gonzalez-Rojo Silvia Fernandez-Diez Cristina Lombo Marta Rodriguez Vanesa Robles Paz Herraez Maria

University of León, León, Spain.

ra0001p362

Conceptus-derived proteins in bovine uterine luminal fluid at the time of pregnancy recognition in cattle

Forde Niamh Bazer Fuller W Spencer Thomas E Lonergan Patrick

UCD, Dublin, Ireland; Texas A&M University, College Station, Texas, USA; Washington State University, Pullman, Washington, USA

ra0001p363

Live imaging reveals novel dynamic events in transitioning mouse gonocytes

Jarvis Sheba Fraser Scott E Winston Robert M Readhead Carol W

Imperial College London, London, UK; University of Southern California, Los Angeles, USA.

ra0001p364

The impact of DNA damage induced by sperm cryopreservation in Xenopus

Morrow Sean Guille Matt Holt William Garner Trent

¹University of Portsmouth, Portsmouth, UK; ²Institute of Zoology, London, UK.

ra0001p365

Sperm total motility and membranes integrity after selection by centrifugation in colloid solutions of ram cryopreserved semen

Gomes Bergstein Tacia Cassia Bicudo Luana Rodello Leandro Romualdo Weiss Romildo Dimas Bicudo Sony

¹State University, Curitiba, Brazil; ²UNESP, Botucatu, Brazil; ³UFPR, Curitiba, Brazil.

ra0001p366

Reproduction Abstracts

World Congress of Reproductive Biology 2014

POSTER PRESENTATIONS

BiosciAbstracts