Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p080 | (1) | WCRB2014

Isolation and characterization of porcine mesenchymal stem cell as a donor for efficient nuclear transfer

Lee Jun Sung , Jung Sung Han , Park Young Bin , Kim Sang Eun , Lee Hoon Taek

Introduction: Nuclear transfer (NT) has used for generating cloned animals or genetically modified animals. However, the efficiency has remained low, because of epigenetic errors that occur during donor cell reprogramming. Mesenchymal stem cells (MSCs) were known as undifferentiated state cell compared to somatic cells. Thus MSCs can reduce the chance of error that can be occurred during reprogramming process and can be easily isolated from adult, while embryonic stem cells ar...

ra0001p083 | (1) | WCRB2014

Comparison of survivin gene expression between porcine SCNT and iSCNT-derived normal and arrested embryos

Choi Kyu Hee , Kim Young Kyu , Choi Seong Deok , Shin Sang Tae , Lee Hoon Taek

Introduction: Interspecies somatic cell nuclear transfer (iSCNT) is an useful tool to produce cloned embryos using livestock oocytes instead of the animal with peculiar reproductive system to produce endangered animal and rescue incurable disease. There are many researches about iSCNT such as porcine-bovine, mouse-porcine, mouse-bovine and so on, in contrast, canine-porcine SCNT especially have been almost no reports.Materials and methods: Porcine fetal ...

ra0001p084 | (1) | WCRB2014

Involvement of poly(ADP-ribosyl)ation and autophagy in porcine pre-implantation development

Lee Hye Ran , Hwang Jeongho , Park Hyo Chang , Han Yong Man , Lee Hoon Taek

Introduction: The poly(ADP-ribosyl)ation (PARylation) has involvement with pro-survival autophagy. However, there has been no report about PARylation and autophagy during pre-implantation development. Here, we investigated the mechanism between PARylation and pro-survival autophagy in pre-implantation development.Materials and methods: IVF embryos were cultured in the medium containing 3-aminobenzamide (3ABA), parp inhibitor. Autophagy and PARylation wer...