Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0003p063 | (1) | SRF2016

Preantral follicle development in cultured reaggregated neonatal ovaries

Lo Belinda , Sheikh Sairah , Williams Suzannah

Introduction: Follicle development is complex, and the use of reaggregated ovaries (ROs) allows us to investigate oocyte-somatic cell interactions, since they can be created using different sources of germ and somatic cells. Production of an RO involves the separation of germ and somatic cells using differential plate adhesion, followed by reaggregation into a pellet. The pellet is then transplanted beneath the kidney capsule of an immunocompromised mouse which facilitates fol...

ra0002o011 | Oral Communications 2: Ovarian function | SRF2015

The role of the oocyte is investigated in premature ovarian failure using the reaggregated ovarian pellet technique

Sheikh Sairah , Galaz Heidy Kaune , Deleva Anna , Williams Suzannah A

Premature ovarian failure (POF) affects around 1% of women and is idiopathic in 74–90% of cases. Our mouse model of POF, the double mutant (DM), are fertile at 6-weeks and undergo POF by 3-months consistent with a decline in developing follicles. However, DM ovaries have increased numbers of primary follicles at the 3a stage, indicating a block in follicle development.We investigated whether we could rescue the ability of DM oocytes at 3- and 9-week...

ra0003o001 | Oral Communications 1: Ovary | SRF2016

Mice with follicular premature ovarian failure at 3 months of age become a follicular by 1 year of age

Plumb Betsy , Sheikh Sairah , Grasa Patricia , Ploutarchou Panayiota , Williams Suzannah

Introduction: Premature ovarian failure (POF) is a reproductive disorder which causes defects within the ovaries, normally leading to infertility. The disorder affects 1% of women under the age of 40 with 75% of cases with no known cause, indicating the need for more research. The Double Mutant (DM) mouse model with oocyte-specific deletion of C1galt1 and Mgat1 exhibit follicular POF at 3 months of age. Therefore, this study aimed to investigate the effect of ovarian dysfuncti...

ra0003p047 | (1) | SRF2016

Germ cells, from a mouse model of Premature Ovarian Failure, retain the potential to support follicle development when reaggregated with wildtype somatic cells

Sheikh Sairah , Kaune Heidy , Deleva Anna , Williams Suzannah

Introduction: Premature ovarian failure (POF) is a condition that affects ~1% of women and is idiopathic in 74–90% of cases. Our mouse model of POF, the Double Mutant (DM), results from oocyte-specific ablation of core 1-derivedO-glycans and complex and hybrid N-glycans. DM females are subfertile at 6-weeks of age and infertile at 9-weeks of age. By 3 months, DM females exhibit POF with ovaries containing fewer developing follicles but more primary 3a follicles. We invest...