Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0003o003 | Oral Communications 1: Ovary | SRF2016

PLCz-induced Ca2+ oscillations are enhanced after germinal vesicle breakdown during mouse oocyte maturation

Sanders Jessica , Bateson Ethan , Yu Yuansong , Nomikos Michail , Lai Antony , Swann Karl

Introduction: Mature mouse oocytes, arrested at meiotic metaphase II (MII), are activated by a series of Ca2+ oscillations caused by sperm-specific phospholipase C zeta (PLCz) which has been shown to localize in cytoplasmic vesicles. The substrate for PLCz, phosphatidylinositol 4,5-bisphosphate (PIP2), is also present in cytoplasmic vesicles in MII oocytes. Throughout oocyte maturation there is reported to be a gradual increase in sensitivity to PLCz and ...

ra0003p056 | (1) | SRF2016

Male infertility-linked point mutation dramatically reduces the Ca2+ oscillation-inducing activity of sperm PLCzeta without affecting its ability to hydrolyse PIP2

Nomikos Michail , Stamatiadis Panagiotis , Sanders Jessica , Calver Brian Lewis , Lofty Morgan , Buntwal Luke , Swann Karl , Lai Francis Anthony

Introduction: Sperm-specific phospholipase C zeta (PLCζ) is widely considered to be the physiological stimulus that evokes intracellular calcium (Ca2+) oscillations that are essential for the initiation of egg activation and early embryonic development during mammalian fertilization. Sperm-delivered PLCζ hydrolyses phosphatidylinositol 4,5-bisphosphate (PIP2) stores within the ooplasm, triggering Ca2+ oscillations through the inositol...

ra0003p057 | (1) | SRF2016

Antigen unmasking improves visualisation efficacy of phospholipase C zeta (PLCζ) in mammalian sperm to enable diagnostic applicability for evaluating PLCζ-dependent human oocyte activation deficiency

Kashir Junaid , Buntwal Luke , Nomikos Michail , Calver Brian , Stamatiadis Panagiotis , Ashley Peter , Sanders David , Knaggs Paul , Bunkheila Adnan , Swann Karl , Lai Francis Anthony

Introduction: Mammalian oocyte activation is mediated via a series of intracellular calcium oscillations induced by a sperm-specific phospholipase C, PLCzeta (PLCζ). PLCζ presents significant promise as a clinical therapeutic for some forms of male infertility. However, the utility of PLCζ as a potent diagnostic tool for human sperm remains undefined. Furthermore, considerable variation in reported PLCζ localization patterns in sperm highlight the necessity...