In fixed time artificial insemination (FTAI) is frozen semen is commonly used, however the use of cooled semen is a practice alternative to reduce costs. The aim of this study was compare the difference in DNA fragmentation and plasma membrane integrity between chilled and frozen semen of bulls. Two ejaculates from 20 bulls were collected. Semen was diluted in extender with egg-yolk and glycerol (Botu-Bov) and divided in two groups: chilled group (CG) samples were cooled at 5 °C for 24 h and frozen group (FG) semen was frozen. Kinect sperm parameters were evaluated by CASA, plasma membrane integrite (PMI, propidium iodate, and FITPSA) DNA fragmentation (DNA, acridine orange) by flow cytometry (BD LSRFortessa). The data were compare using T Test. Total motility (%, CG=89.7±0.9a vs FG=76.3±0.1b), progressive motility (%, CG=65.2±1.7a vs FG=58.2±1.2b), percentage of rapid sperm (%, CG=86.0±1.3a vs FG=71.3±1.7b) and PMI (%, CG=76.5±2.2a vs FG=40.7±1.9b) were higher (P<0.05) in CG compare to FG. DNA (%, CG=1.1±0.1a vs FG=1.6±.1b) was lower (P<0.05) in CG than FG. These results showing that chilled semen in bull provides significantly higher sperm kinect parameters and viability and lower DNA fragmentation in comparison with frozen semen. In conclusion, the use of chilled bovine semen is an important alternative in fixed time artificial insemination.
02 Sep 2014 - 04 Sep 2014