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ISSN 2052-1472 (online)

Reproduction Abstracts (2014) 1 P248 | DOI: 10.1530/repabs.1.P248

Comparision of DNA fragmentantion and plasma membrane integrity between chilled and frozen semen of bulls

Patricia de Mello Papa1, Carlos Ramires Neto2, Priscilla Nascimento Guasti1, Rosiara Rosaria Dias Maziero1, Yame F R Sancler-Silva3, Gabriel A Monteiro3, Camila P Freitas Dell’aqua2, Cassio Renesto Junqueira1, Ian Martin1, Marco Antonio Alvarenga1, Frederico O Papa3 & Jose Antonio Dell’ Aqua Junior1

1Paulo State University, Botucatu, Brazil; 2College of Veterinary Medicine and Animal Science, (UNESP)/Univ Estadual Paulista, Botucatu, Brazil; 3UNESP–BOTUCATU, Botucatu, Brazil.

In fixed time artificial insemination (FTAI) is frozen semen is commonly used, however the use of cooled semen is a practice alternative to reduce costs. The aim of this study was compare the difference in DNA fragmentation and plasma membrane integrity between chilled and frozen semen of bulls. Two ejaculates from 20 bulls were collected. Semen was diluted in extender with egg-yolk and glycerol (Botu-Bov™) and divided in two groups: chilled group (CG) samples were cooled at 5 °C for 24 h and frozen group (FG) semen was frozen. Kinect sperm parameters were evaluated by CASA, plasma membrane integrite (PMI, propidium iodate, and FIT–PSA) DNA fragmentation (DNA, acridine orange) by flow cytometry (BD LSRFortessa). The data were compare using T Test. Total motility (%, CG=89.7±0.9a vs FG=76.3±0.1b), progressive motility (%, CG=65.2±1.7a vs FG=58.2±1.2b), percentage of rapid sperm (%, CG=86.0±1.3a vs FG=71.3±1.7b) and PMI (%, CG=76.5±2.2a vs FG=40.7±1.9b) were higher (P<0.05) in CG compare to FG. DNA (%, CG=1.1±0.1a vs FG=1.6±.1b) was lower (P<0.05) in CG than FG. These results showing that chilled semen in bull provides significantly higher sperm kinect parameters and viability and lower DNA fragmentation in comparison with frozen semen. In conclusion, the use of chilled bovine semen is an important alternative in fixed time artificial insemination.