WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
Atrazine exposure of adult mice affect spermatogenesis
Aurore Gely-Pernot , Chunxiang Hao , Christine Kervarec , Igor Stuparevic , Frédéric Chalmel , Bertrand Evrard , Michael Priming , Bernard Jégou & Fatima Smagulova
Inserm UMR1085, IRSET, Université Rennes1, Rennes, France.
Declining human fertility has become a serious public health problem in many countries around the world. There is a rapidly growing number of evidence that human reproductive health is negatively affected by various environmental factors including life style and exposure to chemical compounds such as pesticides, phthalates, and bisphenol A (BPA). The aim of our study is to understand how commonly used pesticides can affect meiosis, the central event of mammalian gametogenesis. We hypothesize that pesticides change DNA histone modifications and gene expression that finally lead to a decrease of fertility. To test this idea we are using the mouse as a model organism. We treated 4–5 weeks old adult males with the different doses of atrazine during different time intervals. Morphology analysis of the treated mouse showed a reduction of the spermatozoa number in epididymis compared to the control. In some severe cases we found abnormal seminiferous tubules upon atrazine treatment. By using Affymetrix microarray approach we found around 50 genes are differentially expressed including some histone modifying enzymes, transcription factors and regulatory proteins. Immunostaining of tubule sections and chromosomal spreads revealed increased histone gamma H2AX staining which may reflect DNA damage sites or double stranded breaks persistence. Our preliminary data showed that atrazine affect meiosis and have a possible impact on spermatogenesis.
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Reproduction Abstracts
ISSN 2052-1472 (online)
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