Introduction: Lipid content of oocytes and early embryos is critical for embryonic and fetal development. Indeed, the success of assisted reproductive techniques (ARTs) such as cryopreservation and in vitro embryo production is heavily affected by the lipid content of preimplantation embryos.
Materials and methods: Lipid profiling of single in vitro (IVTB) and in vivo (IVVB) produced bovine embryos was performed by desorption electrospray ionization mass spectrometry (DESI MS). The relative abundance of transcripts related to oocyte development capacity (IGF1R and GJA1) and lipid metabolism (FASN, SCAP, SRBP1, and CPT1b) was assessed by RT-qPCR. Similar analyses were performed from oocytes collected from Holstein Friesian Heifers after long-term supplementation with rumen-protected stearic conjugated linoleic (CLA) and stearic (SA) acid of the daily diet. Follicular fluid and blood samples were collected from supplemented animals for monitoring lipid profiles by gas chromatography (GC).
Results and discussion: Results showed effects of in vitro culture conditions on the lipid profile and gene expression of embryos when comparing to their in vivo counterpart. The rumen-protected fatty acid supplementation experiments showed modification of the lipid profile of oocytes collected from supplemented donors. Oocytes collected from CLA supplemented Heifers showed significant accumulation of triacylglycerols of unsaturated fatty while oocytes from the SA supplemented group accumulated higher amounts of palmitic acid and plasmalogen species. These results pave the way for improving embryo culture conditions and for identifying fertility impairments associated to the female nutrition. Results support the role of the bovine experimental model for improving ARTs technologies in humans and other mammals.
02 - 04 Sep 2014
World Congress of Reproductive Biology