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ISSN 2052-1472 (online)

Reproduction Abstracts (2014) 1 P060 | DOI: 10.1530/repabs.1.P060

Thermoprotective action of IGF1 in embryos hamster 4-cells subjected to heat stress in vitro: effect of the redistribution of E-cadherin

Trejo Córdova Alfredo1, Abad Benitez Ismael1, Meza Villalvazo Víctor Manuel1, Navarro Maldonado María del Carmen2 & Ambriz García Demetrio2

1Universidad del Papaloapan, Loma Bonita, Mexico; 2Universidad Autónoma Metropolitana-Iztapalapaa, Distrito Federal, Mexico.

Introduction: IGF1 has been used as a thermoprotective during in vitro culture, however, its function is dependent on the cleavage embryos. Furthermore, the localization of E-cadherin dependent cleavage embryos. It is unknown whether there is a relationship between the thermoprotective action of the IGF1 and localization of E-cadherin.

Materials and methods: Ten female hamster young were used. Hamster embryo 4-cells were obtained. Groups of embryos 4-cells were incubated in the presence or absence of 6- DAMP (compacted and uncompacted). Both groups of embryos were incubated for 24 h under conditions of heat stress. The medium was supplemented with IGF1, the end of the culture period the development stage and the rate of apoptosis was determined.

Results and discussion: The percentage of embryos that reached the 6-cell stage embryos was significantly higher than 4-cells compacted or non-compacted (63.57 vs 38.81 respectively). The intensity of Annexin-V (apoptosis) embryos was significantly higher than non-compacted compacted (33 vs 25 respectively). The anti-apoptotic activity of the IGF1 is via the PI3K/Akt pathway, evidence of a relationship between the activation of this pathway and the formation of cell junctions dependent E-cadherin. In conclusion, the heat protection of the IGF1 activity is favored by a change in the localization of E-cadherin in embryos of four cells when subjected to heat stress in vitro.

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