Despite of the application of numerous supplements to improve in vitro culture (IVC) of mammalian cells, few studies have been conducted concerning the effect of fetal bovine serum (FBS) on early development of mammalian embryos. Thus, the current study was carried out to determine the effect of FBS on the developmental competence of porcine embryos and to establish the optimal treatment conditions. Unlike the treatment during full-term (16 days) or early phase (13 days) of IVC, the beneficial effect of FBS supplementation was only found in late phase (46 days) of IVC. The rates of blastocyst formation and hatching were significantly increased by addition of FBS during the late phase of IVC compared to control, which was further evidenced by the improvement of cellular survival and total cell number in blastocysts. Moremore, reactive oxygen species (ROS) levels were markedly reduced in the late-phase FBS treatment group compared to control. Interestingly, supplementation of hydrogen peroxide aggravated the early development of porcine embryos, which was greatly ameliorated by treatment with FBS during the late phase of IVC. Taken together, these results suggest that FBS can be efficiently used as a useful supplement for massive production of porcine embryos with high developmental competence.
02 - 04 Sep 2014
World Congress of Reproductive Biology