SCNT technology provides potential applications for biomedical and agriculture. However, the efficiency of SCNT is still low. Incomplete epigenetic reprogramming of transferred somatic cell is believed to be one of main causes of developmental problems. Hanwoo is considered as only bovine species with mainly reddish color but quite a few of mini-, white-colored, black-colored, striped cows. The objective of this study was to produce cloned offspring from endangered mini-Hanwoo SCNT embryos reconstructed by using either iPS or AID gene transfected cells. These reprogrammed cells may enhance development of SCNT embryos. biPS cells were prepared from transfection with six reprogramming factors. After the transduction iPS cells were placed in DMEM, LIF and bFGF. pAcGFP1-C1 vectors included AID genes were transfected. Oct4 and Nanog were expressed after transfection of AID gene. The pregnancy of SCNT embryos produced by two cell lines was diagnosed by a ultrasound scanning. Mini-Hanwoo SCNT embryos (blastocysts: n=20) reconstructed by using either iPS or transfected AID cells were transferred into uterus of each synchronized recipients (n=10). One recipient in each group was diagnosed as pregnancy (10 vs 10%). However, control SCNT embryos did not show the pregnancy (blastocysts: n=13; recipients: n=10). This result indicates that bovine SCNT embryos reconstructed by using the reprogrammed cells as iPS and transfected AID cells are able to induce the successful pregnancy.
*This work was carried out with the support of NPR (2011-0013703), Cooperative Research Program for Agriculture Science & Technology Development (PJ009418022014, PJ009117022014) RDA, Korea.
02 - 04 Sep 2014
World Congress of Reproductive Biology