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ISSN 2052-1472 (online)

Reproduction Abstracts (2014) 1 P077 | DOI: 10.1530/repabs.1.P077

Pregnancy of bovine somatic cell nuclear transfer embryos reconstructed by using donor cells: iPS cell vs AID transfected cell*

Tae Suk Kim1, Sang Ki Baek2, Song Yi Moon2, Sang Jin Jin2, Yeoung-Gyu Ko3, Sung Woo Kim3, Hae-Geum Park3, Hwan-Hoo Seong3 & Joon Hee Lee2

1College of Agriculture and Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea; 2Animal Development and Biotechnology Group, Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju, Republic of Korea; 3National Institute of Animal Science, Rural Development Administration, Namwon, Republic of Korea.

SCNT technology provides potential applications for biomedical and agriculture. However, the efficiency of SCNT is still low. Incomplete epigenetic reprogramming of transferred somatic cell is believed to be one of main causes of developmental problems. Hanwoo is considered as only bovine species with mainly reddish color but quite a few of mini-, white-colored, black-colored, striped cows. The objective of this study was to produce cloned offspring from endangered mini-Hanwoo SCNT embryos reconstructed by using either iPS or AID gene transfected cells. These reprogrammed cells may enhance development of SCNT embryos. biPS cells were prepared from transfection with six reprogramming factors. After the transduction iPS cells were placed in DMEM, LIF and bFGF. pAcGFP1-C1 vectors included AID genes were transfected. Oct4 and Nanog were expressed after transfection of AID gene. The pregnancy of SCNT embryos produced by two cell lines was diagnosed by a ultrasound scanning. Mini-Hanwoo SCNT embryos (blastocysts: n=20) reconstructed by using either iPS or transfected AID cells were transferred into uterus of each synchronized recipients (n=10). One recipient in each group was diagnosed as pregnancy (10 vs 10%). However, control SCNT embryos did not show the pregnancy (blastocysts: n=13; recipients: n=10). This result indicates that bovine SCNT embryos reconstructed by using the reprogrammed cells as iPS and transfected AID cells are able to induce the successful pregnancy.

*This work was carried out with the support of NPR (2011-0013703), ‘Cooperative Research Program for Agriculture Science & Technology Development (PJ009418022014, PJ009117022014)’ RDA, Korea.

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