WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
Effect of progesterone levels and parity on the maturity of in vivo canine oocytes
Dong-Hoon Kim , Mi-Kyung Choi , Jin-Gu No , Dong-Hyeon Yeom , Hyun-Min Kim , Dong-Kyo Kim , Byoung-Chul Yang & Jae Gyu Yoo
National Institute of Animal Science, Rural Development Administration, Suwon, Republic of Korea.
Somatic cell nuclear transfer (SCNT) in dogs has been used in vivo-matured oocytes due to low efficiency of in vitro maturation system. Actually, more information is needed to acquire good quality in vivo oocytes for the dog research. The objective of present study was to evaluate whether the progesterone (P4) on estimated ovulation time and the parity of donor bitches have any effect on the maturity of oocytes. In vivo oocytes were retrieved from 289 mixed breed bitchs by laparotomy. Each individual fallopian tube was flushed by Hepes-buffered TCM199 medium containing 10% FBS. Maturity of collected oocytes were categorized as immature, mature, and aged. Our results found a correlation between P4 levels on estimated ovulation time and the parity of donor bitch in the oocyte maturity. In nulliparous bitches, the percentage of mature oocyte was not different among the various P4 levels. However, the percentage of aged oocytes was significantly (P<0.05) increased in 8.01–10.0 and >10.01 ng/ml (22.3 and 18.2%) than in 4.01–6.0 ng/ml (9.6%). In multiparous bitches, the percentage of mature oocyte was significantly (P<0.05) decreased in 6.01–8.0, 8.01–10.0, and >10.01 ng/ml (48.8, 60.7, and 27.1%) than in 4.01–6.0 ng/ml (71.7%). On the contrary, aged oocytes was significantly (P<0.05) increased in 6.01–8.0, 8.01–10.0, and >10.01 ng/ml (31.1, 30.6, and 55.4%) than in 4.01–6.0 ng/ml (18.0%). This study suggests that a correlation between P4 levels on estimated ovulation time and the parity of donor bitch does exist in the maturity of canine oocytes collected by flushing fallopian tube.
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ISSN 2052-1472 (online)
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