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ISSN 2052-1472 (online)

Reproduction Abstracts (2014) 1 P153 | DOI: 10.1530/repabs.1.P153

Effect of different diet fat sources on in vitro maturation of Anglo-Nubian goats oocytes

Ricardo P Nociti, Luciana C Padilha, Luciana D Rola, Felipe F F P C Barros, Ricardo R Uscategui, Renata S G Mariano, Marcus A R Feliciano, Wilter R R Vicente, Maria Emilia F Oliveira & Vera F M H Lima

FCAV/UNESP, Jaboticabal, Brazil.

There is a lack of information about effects of the diet fat source on goat reproduction. The aim of the present study was to evaluate the fat source effect on in vitro maturation (IVM) of oocytes. Eighteen goats were divided into three groups, on diets (40% concentrate/60% corn silage) with 4% (dry matter) of ether extract from different fat sources [soy oil (SG) and linseed (LG), Megalac® (MG)]. The does passed thru an adaptation period (15 days) and, then, an experimental period of 42 days. The laparoscopic ovum pick-up was performed on day 42 of the trial, after 36 h of an ovarian superstimulation protocol [FSH (80 mg) + eCG (300 IU) after 36 h of the last shot of GnRH from the Ovsynch protocol]. The recovered oocytes were classified as viable by morphological appearance. IVM and analysis were performed for each individual. The oocytes were incubated in maturation media (TCM 199 supplemented with 10% inactivated heat goat serum) at 38.5 °C and 5% CO2, 27 h. Oocytes were subsequently fixed and stained on Hoechst 33342 and analyzed using a fluorescent microscope. Data were analyzed by ANOVA. The number of viable oocytes (means±S.E.M) were significantly higher in SG (10.0±2.03) than the others groups (LG: 5.17±1.47 and MG: 5.83±0.48). The nuclear maturation had no difference between groups (SG: 89.0±7.96; LG: 79.3±7.91; and MG: 92.5±4.08). In conclusion, the fat source affect the number of goat viable oocytes, nonetheless, it does not affect the nuclear maturation rate. Financial support: FAPESP.