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ISSN 2052-1472 (online)

Reproduction Abstracts (2014) 1 P176 | DOI: 10.1530/repabs.1.P176

Characterization of pituitary-derived cell lines, Tpit/E, Tpit/F1 and TtT/GF

Naoto Nishimura, Saishu Yoshida, Masashi Higuchi, Hideji Yako, Hiroki Ueharu, Mo Chen, Takako Kato & Yukio Kato

Meiji University, Kanagawa, Japan.

Introduction: The pituitary anterior lobe consists of five types of hormone-producing cells and non-hormonal cells. In non-hormonal cells, Sox2-expressing cells exist as stem/progenitor cells and play a role in the regeneration of hormonal cells. However, elucidation of the differentiation mechanism is yet uncovered. Established cell lines are frequently used as a model system not only for cellular physiology and gene expression but also for cell differentiation. In this study, we compared gene expression profiles of the pituitary-derived non-hormonal cell lines, Tpit/E, Tpit/F1 and TtT/GF.

Materials and methods: Comparison of gene expression profiles by using microarray analysis and real-time PCR for the three cell lines and immunocytochemistry were performed.

Results and discussion: Three lines showed an expression of Sox2 at various degrees. Tpit/E showed the epithelial phenotype by expression of E-cadherin, EpCAM, Krt8 and Snail2. Notably, expression of stem/progenitor marker Sox2, Sox9 and Cxadr, indicate that it has highest stemness among three cell lines. Tpit/F1 expressed marker genes of epithelial and mesenchymal factors in addition to Sox2, suggesting that this cell line is in transiting state of the differentiation or MET. TtT/GF showed the mesenchymal phenotype expressing Vimentin, Prrx1, Prrx2, Nestin and CD34 genes, and might have ability to differentiate into pituitary vascular endothelial cells or pericyte. These data suggest that these three cell lines are useful model to investigte of pituitary stem/progenitor cells as well as pituitary organogenesis.

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