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ISSN 2052-1472 (online)

Reproduction Abstracts (2014) 1 P198 | DOI: 10.1530/repabs.1.P198

Effect of storage time on Atlantic salmon (Salmo salar) sperm motility, viability and calcium levels, first communication

Jorge Parodi

Universidad Catolica de Temuco, Temuco, Chile.

Few studies have evaluated sperm function and intracellular calcium levels when storage of semen for a long period of time. We have assessed sperm viability, morphology, motility and intracellular calcium levels in fish semen after a prolonged storage period. Semen samples from Atlantic salmon were preserved at 4 °C for 25 days and then evaluated for viability. The motile sperm was determined using the Image J-CASA program. To measure the calcium content, the sperm were loaded with a calcium fluorescent probe and evaluated by confocal microscopy and microfluorimetry. Reduced sperm viability was observed after 10 days of storage. Motility remained high for the first 3 days. A decrease in the calcium content of more than 50% was observed after 20 days of storage. When sperm were activated, calcium levels increased over 200% of relative fluorescence units (RFU); this increase was lost when the samples were stored for extended periods and only partially manifested in a zero calcium solution. Our results suggest that in vitro storage of Atlantic salmon sperm at 4 °C for a period of 3 days preserves viability and motility at levels similar to those of fresh sperm and also maintains intracellular calcium stores, which are critical for sperm function.

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