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ISSN 2052-1472 (online)

Reproduction Abstracts (2014) 1 P263 | DOI: 10.1530/repabs.1.P263

Comparison between egg yolk-based and coconut water-based extenders to freeze buffalo semen

Mariana Zorzetto, Yamê Sancler-Silva, Camila Freitas Dell’aqua, Carlos Ramires Neto, Saulo Zocca, Alcides Ramos, Eunice Oba & Ana Augusta Pagnano Derussi


Unesp Botucatu, Botucatu, Brazil


The aim of this experiment was compare two egg yolk-based (Tris and botu-Bov) and a coconut water-based (ACP-111) extenders to freeze buffalo semen. Four ejaculates of four buffalo bull were collected. Semen was divided in three groups: EY1 group, the semen was diluted with Tris-Yolk extender; EY2 group, the semen was diluted with Botu-Bov TM; CW group, the semen was diluted with ACP-111TM. The samples were packed in 0.5 ml straws containing 30×106 of total sperm and were freezing in isothermal box. The sperm kinetics was evaluated by CASA method (HTM-IVOS, IMV, USA) and the plasma membrane integrity and acrosomal (probes propidium iodide and FITC-PSA) were analyzed by flow cytometer (BD LSR Fortessa, BD, Mountain, USA). The evaluations were performed post-thaw. Comparison of sperm parameters was performed by ANOVA. The values were expressed as mean and standard error. CW was smaller (P<0.05) than EY1 and EY2 in total motility (%, EY1=52.64±3.15a; EY2=58.59±3.56a; CW=36.94±4.05b), progressive motility (%, EY1=40.39±2.58a; EY2=47.56±2.82a; CW=29.59±3.34b), and percentage of rapid sperm (%, EY1=48.29±3.17a; EY2=55.47±3.49a; CW=34.06±3.94b). No difference (P>0.05) was observed in IMP (%, EY1=25.90±2.14a; EY2=29.72±2.39a; CW=24.37±2.34a), The results of this study allow us to conclude that egg yolk-based extenders showed to be more efficient than coconut water-based extender to freeze buffalo semen.

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