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ISSN 2052-1472 (online)

Reproduction Abstracts (2014) 1 P296 | DOI: 10.1530/repabs.1.P296

Evaluation of protein profile in uterine secretion on early cyclic and gestational diestrus in bitches

Ana Augusta Pagnano Derussi1, Rodrigo Volpato2, Carlos Renato de Freitas Guaitolini2, Camila Louise Ackermann2, Marilda Onghero Taffarel3, Guilherme Schiess Cardoso4, Ana Izabel Silva Balbin Villaverde1, Fabiana Ferreira de Souza1 & Maria Denise Lopes2

1School of Veterinary Medicine and Animal Science- UNESP- Botucatu, Botucatu, Brazil; 2São Paulo State University, Botucatu, Brazil; 3State University of Maringa, Maringa, Brazil; 4State University of Londrina, Londrina, Brazil.

Introduction: During peri-implantation period the canines conceptus are nourished by secretions in uterine lumen, a phenomenon known as histotrophic nutrition, in which several proteins are present. The aim of this study was to compare the protein profile in uterine secretion in early pregnancy and early diestrus cyclic using two-dimensional electrophoresis technique.

Material and methods: For that, ten bitches were submitted to OHE on the 12th day after the preovulatory LH surge. Five females were in early diestrus cyclic (Group IA) and the others were in gestational diestrus (group IB). The samples were obtained by uterine flushing using PBS solution and resulting wash was storage at −20 °C. We prepared pools with those samples, each one containing five samples of the respective groups. Two-dimensional electrophoresis was performed with 138.40 μg /μl of protein per strip (7 cm) and four replicates per group. The gels were stained using Coomassie G-250, scanned and interpreted using an specific software (Image Master version 7.0, GE HealthCare, Upsalla, SE).

Results and discussion: Thirty three spots were expressed in group A and 29 spots in group IB. Three proteins were expressed exclusively in non pregnant bitches (group IA) and eight proteins were exclusively expressed during early pregnancy (group IB), this fact proves the existence of a distinct protein secretion in canine uterus on early pregnancy. Those exclusive proteins in gestation may have an important potential in precoce diagnosis of pregnancy in bitches, as well as being involved in embryo implantation process.