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ISSN 2052-1472 (online)

Reproduction Abstracts (2014) 1 P318 | DOI: 10.1530/repabs.1.P318

Transmission of lethal phenotype in a Mendelian fashion by genetically modified pigs that underwent blastocyst complementation

Hitomi Matsunari1, Masahito Watanabe1, Kazuhiro Umeyama1, Kazuaki Nakano2, Masaki Nagaya1, Hiromitsu Nakauchi3 & Hiroshi Nagashima1

1Meiji University International Institute for Bio-Resource Research (MUIIBR), Kawasaki, Japan; 2Laboratory of Developmental Engineering, Department of Life Sciences, Meiji University, Kawasaki, Japan; 3Institute of Medical Science, The University of Tokyo, Tokyo, Japan.

In our previous study, we demonstrated that pigs expressing neonatally lethal phenotype induced by genetic modification could be rescued by induction of chimerism by using blastocyst complementation. Here, we show that the obtained chimeric pigs can faithfully transmit the genotype, which is the cause of the lethal traits, to progenies.

Cloned embryos (male) of pigs showing the phenotype of pancreatogenesis deficiency by expression of the Pdx1-Hes1 gene were complemented with cloned embryos derived from three types of female pigs (humanized Kusabira-Orange transgenic, Pdx1-Venus transgenic, and coat colored WT) to produce six chimeric boars.

Except for one, all chimeric boars grew normally and reached sexual maturity. A total of 120 fetuses and piglets were produced by mating the five chimeric boars with 12 WT females. Analysis of 82 fetuses (day 47–109) and 38 piglets showed that 61 (50.8%) were Pdx1-Hes1 transgenic, which inherited the phenotype of pancreatogenesis deficiency. The average birth weight of the transgenic piglets was significantly lighter than that of the non-transgenic piglets (906.5±40.8 g vs 1247.1±41.7 g; P<0.01). All transgenic piglets showed a high glucose level of 400 mg/dl or more, and died by several days. Our results demonstrated that the chimeric boars produced by blastocysts complementation of Pdx1-Hes1 transgenic-cloned embryos sired fetuses/piglets with the apancreatic phenotype in a Mendelian fashion. Thus, fetuses/piglets expressing lethal traits such as organogenesis deficiency are potential and powerful tools for research on regenerative medicine. This study was supported by JST, ERATO, Nakauchi Stem Cell and Organ Regeneration Project, and Meiji University International Institute for Bio-Resource Research (MUIIBR).

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