WCRB2014 POSTER PRESENTATIONS (1) (335 abstracts)
Effects of common FSH receptor single-nucleotide polymorphisms on the follicular fluid hormone profile and the granulosa cell gene expression in human small antral follicles
Tanni Borgbo 1 , Hana Kluckova 2 , Milan MacEk 2 , Jana Chrudimska 2 , Janni V Jeppesen 3 , Ida Lindgren 4 , Yvonne Lundberg Giwercman 4 , Lise Lotte Hansen 5 & Claus Yding Andersen 1
1Rigshospitalet, Copenhagen, Denmark; 2University Hospital Motol, 2nd Faculty of Medicine, Charles University, Prague, Czech Republic; 3University Hospital of Copenhagen, Copenhagen Ø, Denmark; 4Lund University, Malmø, Sweden; 5Aarhus University, Aarhus C, Denmark.
Background: FSH is one of the key hormones of mammalian reproduction. FSH signaling may be affected by single-nucleotide polymorphisms residing in the FSH receptor (FSHR) gene. The most pronounced effects of these polymorphisms are likely to be displayed in the follicle fluid, which acts as a reservoir for granulosa cell secreted hormones induced by FSH.
Methods: Genetic polymorphisms of FSHR in position -29 (FSHR -29) and in codon 307 and codon 680 (FSHR307/680) were correlated to measurements of intrafollicular levels of AMH, estradiol, progesterone, testosterone, and androstenedione. Furthermore, the genotypes of FSHR307/680 were correlated to granulosa cell gene expression of FSHR, LHR, CYP19a1, AR, AMH, and AMHR2.
Results: For FSHR307/680, significant genotype specific differences were observed for AMH, estradiol, and testosterone. The GG genotype had significantly higher AMH levels than the AG genotype, especially in the group of follicles with a diameter of 3–6 mm. In follicles exceeding 6 mm, AMH levels were highest in the GG group in parallel with levels of estradiol, progesterone, and testosterone. The gene expression profiles showed significantly elevated levels of LHR for the GG genotype, with similar patterns observed for FSHR and CYP19a1. For FSHR -29, the concentration of AMH and testosterone was higher for the AA genotype, while an inverse profile was observed for estradiol. The greatest effects were exerted by FSHR -29 in comparison to FSHR307/680.
Conclusion: Significant changes in the granulosa cell function of human small antral follicles can be observed as effects of the FSHR polymorphisms FSHR −29 and FSHR307/680.
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Reproduction Abstracts
ISSN 2052-1472 (online)
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