Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0002p048 | (1) | SRF2015

The effect of prenatal exposure to androgen and to a high fat diet on obesity in female mice

Laird Mhairi , Hardy Kate , Franks Stephen

Polycystic ovary syndrome (PCOS) is a complex endocrine disorder that presents in women of reproductive age. The aetiology of PCOS is poorly understood, however it is likely that women with this disorder are predisposed to produce excess androgen at or well before puberty. Environmental and lifestyle factors then contribute to the multifarious symptoms observed. One such key external factor is diet. These studies utilised a prenatally exposed androgen mouse model to investigat...

ra0001p160 | (1) | WCRB2014

Androgen action within the preantral follicle; a role for the epidermal growth factor receptor?

Thomson Kacie , Laird Mhairi , Franks Stephen , Hardy Kate

Introduction: Polycystic ovary syndrome (PCOS) is the most common cause of anovulatory infertility affecting over 5% of the female population. PCOS is characterized by aberrant early follicle development in which hyperandrogenism is thought to play a key role. However, the molecular mechanisms of androgen action within the ovary remain largely unknown. Recent evidence suggests androgens may be acting, in part, through modulation of growth factor signalling. We have recently sh...

ra0001p161 | (1) | WCRB2014

Differential role of TGFβ2 during follicle activation and preantral follicle growth

Oliver Elizabeth , Laird Mhairi , Franks Stephen , Hardy Kate

Introduction: Female mammals are born with a finite reserve of primordial follicles which dictates the reproductive lifespan. Despite this the factors responsible for primordial follicle activation remain largely unknown. The transforming growth factor beta (TGFβ) superfamily has been implicated in early follicle development however little attention has been given to the effects of TGFβ2, expression of which has been demonstrated in the ovary. This study aimed to exa...

ra0002o012 | Oral Communications 2: Ovarian function | SRF2015

Differential effects of transforming growth factor beta 2 in two different models of preantral follicle growth

Oliver Elizabeth , Laird Mhairi , Franks Stephen , Hardy Kate

The onset of mammalian follicle development is marked by granulosa cell (GC) proliferation, GC shape change, and oocyte growth. Despite their critical role in female fertility the factors and mechanisms underlying these cellular changes remain largely unknown. The transforming growth factor beta (TGFβ) superfamily has been strongly implicated in early follicle growth. Disruption of the function or expression of members of the TGFβ superfamily, receptors or key compon...

ra0003p006 | (1) | SRF2016

Does Neuromedin B exert a local modulatory effect on ovarian steroidogenesis or cell proliferation?

Mattar Dareen , Laird Mhairi , Samir Moafaq , Cheewasopit Warakorn , Knight Phil

Introduction: Neuromedin B (NMB), a highly conserved bombesin-related decapeptide originally isolated from porcine spinal cord, has various physiological effects including the regulation of exocrine and endocrine secretions. The current aims were to investigate whether: (1) NMB is expressed in the bovine ovary (2) NMB or NMB antagonist can modulate ovarian steroidogenesis or proliferation by cultured bovine theca (TC) and granulosa (GC) cells.Methods: GC...

ra0002o016 | SRF Student Prize Session | SRF2015

Investigating a role for the epidermal growth factor receptor in androgen signalling within mouse preantral follicle development

Thomson Kacie , Mora Jocelyn M , Laird Mhairi , Franks Stephen , Hardy Kate

Hyperandrogenism and dysregulated follicle development both characterise polycystic ovary syndrome (PCOS), however little is known about how androgens affect early preantral follicle development. Recent evidence suggests that androgens may act, in part, through non-classical modulation of growth factor signalling pathways. Members of the epidermal growth factor (EGF) family play a role in promoting preantral follicle development in the mouse, with receptor subtypes EGFR, ErbB2...

ra0002p035 | (1) | SRF2015

Differential gene expression in granulosa–lutein cells from women with polycystic ovaries is independent of the dose of FSH given for ovarian stimulation

Velupillai Jocelyne , Lerner Avi , Christopoulos Georgios , Liyanage Maneshka , Lavery Stuart , Trew Geoffrey , Laird Mhairi , Mora Jocelyn M , Tsui Victoria , Hardy Kate , Franks Stephen

Polycystic ovary syndrome (PCOS) affects over 5% of women of reproductive age. It is associated with an ovulatory infertility, menstrual disturbances as well as metabolic abnormalities including obesity and insulin resistance. PCOS has a strong genetic basis but studies of ovarian gene expression in PCOS are limited by the difficulty in obtaining tissue samples from women with and without PCOS. For that reason, most studies have been performed in granulosa–lutein (GL) cel...