Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001s028 | Control of meiosis | WCRB2014

Making it through meiosis: APC/C FZR1 has an essential role in meiotic prophase I in germ cell development

McLaughlin Eileen A

Fizzy-related 1 (FZR1) is one of two known activators of the anaphase promoting complex (APC) and a well-established important regulator of the mitotic cell cycle. In a germ-cell-specific (DDX4-cre) conditional knockout model we examined the role of FZR1 in germ cell development with particular emphasis on the entry into meiosis and early meiotic events. Loss of APC FZR1 activity in the male germline led to both a mitotic and a meiotic cell defect resulting in sterility throug...

ra0001p201 | (1) | WCRB2014

Alternative splicing: a mechanism for spermatogonia differentiation, meiosis progression and spermatid maturation

Jamsai Duangporn , Clark Brett , McLaughlin Eileen , O'Bryan Moira

Introduction: The production of functional spermatozoa is a highly complex process that requires tight regulation of gene expression. Pre-mRNA splicing is an essential post-transcriptional process. Over 95% of multi-exon human genes undergo alternative splicing to create diverse arrays of mRNAs from a single gene. Disturbance of splicing regulation is associated with many human diseases.Materials and methods: A genome-wide ENU mutagenesis screen was carr...

ra0001p222 | (1) | WCRB2014

The RNA-binding protein Musashi-2 (MSI2) controls mRNA processing and translational regulation via interactions with SFPQ and PIWIL1 during mammalian spermatogenesis

Sutherland Jessie M , Sobinoff Alexander P , Redgrove Kate , Davidson Tara-Lynne , Siddall Nicole A , Koopman Peter , Hime Gary R , McLaughlin Eileen

Introduction: RNA-binding proteins (RBP) are important catalysts of post-transcriptional gene regulation. The RBP MSI2 directs stem cell function and division through established roles in cell cycle dynamics and cell fate determination. During the complex process of mammalian spermatogenesis, the directed uncoupling of transcription and translation is essential for the correct development of male gametes. We have previously established that dysregulation of MSI2 during germ ce...

ra0002s004 | SRF-SRB Exchange Lecture | SRF2015

SRF–SRB Exchange Lecture: Musashi RNA binding protein MSI2 interacts with SFPQ and controls the expression of target mRNAs Tbx1 and Piwil1 in male germ cells during spermatogenesis

Sutherland Jessie M , Fraser Barbara A , Sobinoff Alexander P , Siddall Nicole A , Koopman Peter , Hime Gary R , McLaughlin Eileen A

Controlled gene regulation during gamete development is vital for maintaining reproductive potential. During the process of gamete development, male germ cells experience extended periods of inactive transcription despite requirements for continued growth and differentiation. Spermatogenesis therefore provides an ideal model to study the effects of post-transcriptional control on gene regulation. During spermatogenesis post-transcriptional regulation is orchestrated by abundan...