Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0002o007 | Oral Communications 1: Embryo and Implantation | SRF2015

A potential co-culture system of ovine blastocyst with uterine endometrium to mimic initial attachment of embryo implantation

Tremaine Tina , Fouladi-Nashta Ali A , Wathes D Claire , Khalid Muhammad

In livestock ruminant species including sheep and cattle, high fertilisation rates shown around 80%, do not necessarily equate to successful pregnancy. Deficient uterine function is therefore a major contributory factor to pregnancy failure resulting in embryonic mortality. In humans undergoing IVF treatment for subfertility, approximately only 25% of embryo transfers will successfully implant. To gain a better understanding of the natural implantation environment, in vitr...

ra0002p002 | (1) | SRF2015

Associations between circulating non-esterified fatty acids and uterine function in postpartum dairy cows

Cheng Zhangrui , Hiu Yiu Yao Veronica , Wathes D Claire

Evidence suggests that excessive non-esterified fatty acid (NEFA) production during postpartum negative energy balance (NEB) increases the risk of uterine infection. The aims of our study were to investigate associations between circulating NEFAs and uterine gene expression in postpartum dairy cows with NEB using microarray and bioinformatics techniques. Mild NEB (n=6) and severe NEB (n=6) in 12 cows were produced using different milking and...

ra0001p293 | (1) | WCRB2014

Non-cytopathic BVDV suppressed innate immune response in bovine endometrial cells exposed to bacterial lipopolysaccharide

Oguejiofor Chike Fidelis , Cheng Zhangrui , Anstaett Olivia , Brownlie Joe , Fouladi-Nashta Ali A. , Wathes D Claire

Introduction: Infection with non-cytopathic BVDV (ncpBVDV) is associated with uterine disease and infertility in dairy cows although the underlying mechanisms are poorly understood. This project investigated the influence of ncpBVDV on innate immunity of the bovine endometrium.Materials and methods: Primary bovine endometrial cells from each of six cows were divided into four groups (Control; LPS; BVDV; BVDV LPS). Cells were infected with ncpBVDV for 4 d...

ra0002p029 | (1) | SRF2015

Successful isolation, culture and karyotyping of equine placental cells from failed early pregnancies

Rose Belinda Vivienne , Cabrera-Sharp Victoria , Cameron Ian , Crabtree James , Crowhurst James , Firth Marvin , Ghosh Sharmila , McGladdery Andrew , Neal Huw , Pynn Jan , Pynn Oliver , Raudsepp Terje , Smith Charlie , Verheyen Kristien , Wathes D Claire , Wise Zara , de Mestre Amanda

Early pregnancy loss (EPL) in the mare is defined as loss of pregnancy between initial detection and day 65 of gestation. It occurs in 7–10% of pregnancies and yet little is known about the underlying pathologies. A lack of suitable conceptus material has limited investigation into the role of chromosomal defects in EPL. The objective of this study was to develop a method to isolate and culture placental cells isolated from failed pregnancies to enable further genetic cha...