Banner advert

ISSN 2052-1472 (online)

Reproduction Abstracts (2014) 1 P131 | DOI: 10.1530/repabs.1.P131

Inhibin [alpha] induced porcine granulosa cells apoptosis through mitochondrial apoptotic pathway

Wan-Hong Li, Shu-Xiong Chen, Lu Chen, Chunjin Li & Xu Zhou

Jilin University, Changchun, China.

Inhibin is a heterodimeric gonadal glycoprotein hormone belonging to the TGFβ superfamily that named for its ability to suppress FSH synthesis and secretion from anterior pituitary via a negative feedback. It can regulate proliferation, differentiation, and steroidogenesis of granulosa cells (GCs) through paracrine and autocrine manners. The objective of this study was to investigate the interaction between inhibin α subunit (INHA) and apoptosis of porcine ovarian GCs by exploring i) the effect of INHA on mitochondrial membrane potential (MMP) of GCs and ii) the effect of INHA on Caspase-3 and Bax mRAN expression in GCs. In experiment 1, GCs were divided into two groups and cultured in serum-free medium for 12 h with 0 and 50 ng/ml INHA. Then, MMP were detected under fluorescence microscopy using mitochondrial membrane potential assay kit with JC-1. Treatment with INHA induced a disappearance of red fluorescence along with a diffusion of green fluorescence into the cytoplasm of GCs. This shift of JC-1 fluorescence from red to green indicated a collapse of MMP. In experiment 2, GCs were treated with different concentration of INHA (0, 10, 20, 50, and 100 ng/ml) for 6 and 18 h. Then, total RNA was extracted, Caspase-3 and Bax mRNA were detected by qRT-RCR. The expression levels of Bax and caspase-3 were significantly increased (P<0.05) after INHA treated for 6 h. For 18 h, only treatment with 50 ng/ml INHA significantly (P<0.05) increased Caspase-3 expression. In conclusion, INHA may have a role in regulating the apoptosis of GCs through mitochondrial apoptotic pathway. This research was supported by the State Key Development Program of Basic Researchof China (No. 2011CB944203).

Article tools

My recent searches

No recent searches.