Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p138 | (1) | WCRB2014

The development of a novel mouse embryonic ovary culture

Stefansdottir Agnes , Adams Ian , Spears Norah

Introduction: In vitro cultures are a widely used tool to study ovary development and assess reproductive toxicology of chemicals. However, establishing a culture system whereby mouse ovaries can be cultured from a pre-meiotic stage to a mature oocyte has proved challenging. We have developed a novel culture system that spans meiotic entry to meiotic arrest, germ cell nest break-down, follicle formation, and initiation of follicle growth.Methods...

ra0001p148 | (1) | WCRB2014

Cell viability in ovarian follicles exposed to cisplatin and doxorubicin

Kirkwood Phoebe Maud , Lopes Federica , Spears Norah

Introduction: This work adapts a novel ovary culture technique, devised by De Felici’s group, for assessing the ovotoxicity of two chemotherapeutic drugs, cisplatin and doxorubicin. Previous work from our laboratory used cultures of intact neonatal ovaries to determine the effects of the two drugs, showing that both cisplatin and doxorubicin are moderately ovotoxic.Methods: Here, ovaries collected from newborn WT mice were cultured as by De Felici. ...

ra0001p144 | (1) | WCRB2014

Etoposide has a detrimental impact on mouse ovarian development when exposure occurs during early meiotic prophase

Johnston Zoe C , Stefansdottir Agnes , Adams Ian , Spears Norah

Introduction: The use of the chemotherapeutic agent etoposide in pregnancy is considered to be relatively safe during the second and third trimesters. However, the drug does have detrimental effects on oocytes undergoing meiosis II. Similar effects on oocytes in meiosis I may have a clinical impact on the fertility of women exposed to the drug in utero, during critical stages of ovarian development. This study aims to examine the effects of etoposide exposure during e...

ra0003p024 | (1) | SRF2016

Therapeutic doses of phosphoramide mustard cause germ cell death in the prepubertal mouse testis

Rice Siobhan , Smart Ellie , Lopes Federica , Mitchell Rod , Spears Norah

The past few decades have seen marked improvements in life expectancy following childhood cancer due, in part, to advances in chemotherapy. While these drugs are effective in treating malignant disease, one of the main adverse outcomes can be infertility. This is of particular concern for prepubertal boys, for whom there are not yet any established methods of fertility preservation. The objective of this study was to gain a better understanding of the effects of the widely-use...

ra0003o014 | SRF Post-Doctoral Prize Session | SRF2016

Etoposide results in follicle loss in the fetal mouse ovary, but does not block the ability of oocytes to progress through prophase I of meiosis

Stefansdottir Agnes , Johnston Zoe , Powles-Glover Nicola , Anderson Richard , Adams Ian , Spears Norah

Introduction: The chemotherapy agent etoposide is a topoisomerase II (topo II) inhibitor, and is considered safe to administer during pregnancy. However, assessment of its effects on the developing ovary, when germ cells are undergoing initiation of meiosis and forming follicles, has been limited. We have investigated this using ovarian tissue culture.Methods: E13.5 mouse ovaries were cultured for 12 days on an agar block, with etoposide added for the fi...

ra0003p020 | (1) | SRF2016

Effects of the chemotherapy drugs cisplatin and doxorubicin on the follicles of the human ovary

Liu Jin , Lopes Federica , Morgan Stephanie , Nevin Lucy , Telfer Evelyn , Anderson Richard A , Spears Norah

Introduction: The effect of chemotherapy treatment on premenopausal women’s fertility has long been of concern. Here, we investigate the direct actions of chemotherapy drugs on the human ovary, examining effects of two commonly used drugs, cisplatin and doxorubicin.Method: Ovarian cortical tissue samples were collected from patients aged 27–34 who were undergoing elective caesarean section, with informed consent and Ethical Committee approval. ...