Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0002s012 | Symposia 3: Management of livestock fertility | SRF2015

Impact of prenatal stress on reproductive development in livestock

Ashworth Cheryl J , Hogg Charis O , Rutherford Kenneth M D

Many studies demonstrating that the environment a pregnant female experiences can have profound and sometimes persistent effects on offspring development use extreme experimental perturbations which do not reflect the range of environments a pregnant female is actually likely to experience. We have assessed offspring reproductive development following treatments designed to reflect husbandry and management conditions that pregnant sheep or pigs encounter. Female offspring of s...

ra0003p007 | (1) | SRF2016

Alterations in the vasculature of placental but not endometrial tissue associated with small porcine foetuses compared to their normal-sized littermates

Stenhouse Claire , Hogg Charis , Donadeu Xavier , Ashworth Cheryl

Introduction: Low piglet birth weight has severe consequences for neonatal and adult development that cannot be remedied post-natally. It is hypothesised that impaired foetal growth occurs due to inadequate placental vascularisation.Methods: Endometrial and placental tissues supplying small and normal-sized foetuses were collected from Large White x Landrace gilts at gestational days (GD) 30 (n=5), 60 (n=7) and 90 (n=5) for imm...

ra0001p108 | (1) | WCRB2014

Effect of the way in which ewes were handled during mid-gestation on testicular and ovarian development in their lambs

de Jonge Suzanne , Hogg Charis O. , Baxter Emma , Hall Sarah , Dwyer Cathy M. , Ashworth Cheryl Joy

Introduction: This study determined whether the way in which pregnant ewes were handled affected gonad development in their offspring.Methods: Twin-bearing Scottish Blackface × Border Leicester ewes were either handled minimally (control, C, n=20), positively (predictable timings, soft vocal tones etc. P, n=20) or negatively (random timings, shouting and whistling etc. N, n=20) between day 65 and 100 of pregnancy. Followin...

ra0001p155 | (1) | WCRB2014

Proteins in porcine follicular fluid as potential biomarkers for fertility

Jarrett Selene , Gill Andy C , Kurian Dominic , Hogg Charis O , Ferguson Elizabeth M , Ashworth Cheryl Joy

Introduction: Following IVF, blastocysts produced from oocytes recovered from gilts fed a high fibre diet for the preceding 19 days contained more cells than blastocysts from oocytes of control-fed gilts. Oocytes were collected on day 19 of the gilts’ third oestrous cycle and matured in 10% of their own follicular fluid (FF), suggesting that FF may confer the reproductive benefits. The current study compared the protein composition of pooled FF from six high fibre-fed pig...

ra0001p301 | (1) | WCRB2014

Secreted phosphoprotein 1 (SPP1), vascular endothelial growth factor A (VEGFA), heparinase (HPSE), hypoxia-inducible factor (HIF1A) and uteroferrin (ACP5) gene expression in uterine and placental tissues from Large White-Landrace (LW-LD), hyperprolific Large White (hLW) and Meishan (MS) pigs

Hernandez Silvia C , Hogg Charis O , Ait-Ali Tahar , Ashworth Cheryl Joy

Introduction: This study compared uterine and placental expression of genes implicated in placental attachment and angiogenesis in two prolific (hLW and MS) and one control (LW-LD) pig breeds.Methods: Placental and endometrial tissues supplying the smallest and a normal-sized foetus within the same litter were collected from five LW-LD, five hLW and four MS gilts on days 40–45 of pregnancy. RNA was isolated and qPCR for SPP1, VEGFA...

ra0002o018 | SRF Student Prize Session | SRF2015

Variations in the way UK pregnant sheep are managed programs male reproductive development

Moutevelidi Evangelia S , Hogg Charis O , Rutherford Kenneth M D , Ashworth Cheryl J

This study determined whether variation (representative of normal UK farm conditions) in pregnant ewe management is sufficient to alter the programing of the male reproductive axis. Between days 85 and 138 of pregnancy, twin-bearing ewes were managed to either mimic ‘poor husbandry’ (PH) with a high stocking density, reduced feeding space and repeated social mixing or according to ‘best practice’ (BP) with a lower stocking density, longer feed troughs, and ...

ra0002o027 | Oral Communications 4: Female reproduction | SRF2015

Investigation into the role of endometrial heparinase, hypoxia-inducible factor 1A, secreted phosphoprotein 1, uteroferrin, and vascular endothelial growth factor A in foetal growth in pigs

Stenhouse Claire , Hogg Charis O , Donadeu Francesc X , Ashworth Cheryl J

Low birth weight observed in the ‘runt’ piglet of the litter compared to its siblings has severe consequences for neonatal and adult development that cannot be remedied post-natally. This study compared the expression of a number of candidates hypothesised to be involved in angiogenesis and placental attachment in porcine endometrial samples.Endometrial tissues supplying the smallest and normal-sized foetuses were collected from large white lan...

ra0003p051 | (1) | SRF2016

The effect of macrophage colony-stimulating factor (CSF1) on piglet gonad development

Ashworth Cheryl , Avey Billie , Stenhouse Claire , Sauter Kristin , Waddell Lindsey , Hogg Charis , Lisowski Zofia , Hume David

Introduction: CSF1 is the primary growth factor required for macrophage proliferation and differentiation. In adults, the receptor is expressed only in macrophages and their precursors. In mice, mutation of the CSF1 gene produces both male and female infertility due to diminished gonadal steroid production. We tested the effect of increased availability of CSF1 on gonadal development in pigs using CSF1-Fc, a CSF1 fusion protein with increased circulating half-life.<p class...